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[183538_at]

Description T22F7.1 /REP_DB=WormBase Gene ID /WP=CE01416 /TR=Q22686 /GB=AAA20671.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 16      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 16      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 343
1 2 3 4 range
A 583
(3)
P 		277.8
(-2.3)
P 		247.9
(-2.9)
P 		240.5
(-3)
P 		343
B 556.7
(3)
P 		367.3
(-1.1)
P 		276.5
(-3)
P 		302.5
(-2.4)
P 		280
C 572.1
(3)
P 		313.3
(-1.8)
P 		248.4
(-3)
P 		275.2
(-2.5)
P 		324
avr 570.6
(3) 		319.5
(-1.8) 		257.6
(-3) 		272.7
(-2.7) 		313

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 456
1 2 3 4 5 6 range
A 684.4
(3)
P 		439.7
(-0.7)
P 		381.6
(-1.5)
P 		319.2
(-2.5)
P 		286.8
(-3)
P 		285
(-3)
P 		399
B 531.5
(3)
P 		435.2
(0.5)
P 		400.1
(-0.4)
P 		300.3
(-3)
P 		309.6
(-2.8)
P 		309.7
(-2.8)
P 		231
C 520.2
(3)
P 		480.7
(2.2)
P 		323.9
(-1)
P 		307.3
(-1.4)
P 		228
(-3)
P 		299.9
(-1.5)
P 		292
avr 578.7
(3) 		451.9
(0.5) 		368.5
(-1.2) 		308.9
(-2.3) 		274.8
(-3) 		298.2
(-2.5) 		304
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 212.3
(3)
P 		60.3
(-2.1)
P 		64.8
(-2)
P 		35.2
(-3)
P 		177
B 177.5
(3)
P 		90.7
(-1.6)
P 		64.1
(-3)
P 		64.5
(-3)
P 		113
C 189.4
(3)
P 		90.1
(-1.2)
P 		86.6
(-1.3)
P 		46.4
(-3)
P 		143
avr 193.1
(3) 		80.4
(-1.7) 		71.8
(-2) 		48.7
(-3) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 126.2
(0.9)
P 		156.4
(3)
P 		83.1
(-2.1)
P 		81
(-2.2)
P 		86.5
(-1.8)
P 		69.4
(-3)
P 		87
B 184.9
(3)
P 		144.9
(0.7)
P 		97.2
(-2)
P 		79.5
(-3)
P 		125.8
(-0.4)
P 		115.5
(-1)
P 		105
C 192.8
(3)
P 		146.7
(0.9)
P 		82.4
(-2.1)
P 		61.8
(-3)
P 		73.8
(-2.5)
P 		100.4
(-1.2)
P 		131
avr 168
(3) 		149.3
(1.8) 		87.6
(-2.1) 		74.1
(-3) 		95.4
(-1.6) 		95.1
(-1.7) 		94
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183538_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8182
Sequence Type Exemplar sequence
Representative Public ID T22F7.1 NCBI
Target Description T22F7.1 /REP_DB=WormBase Gene ID /WP=CE01416 /TR=Q22686 /GB=AAA20671.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064808(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064808 NCBI Caenorhabditis elegans T22F7.1 (T22F7.1) mRNA, complete cds. 11/11 None
SNAP00000006223 ENSEMBL cdna:SNAP chromosome:CEL140:III:542125:546371:1 11/11 None
T22F7.1 ENSEMBL cdna:known chromosome:CEL140:III:542064:546508:1 gene:T22F7.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000006226 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:542124-546371(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T22F7.1
Entrez Gene 3564822 Entrez gene
SwissProt Q22686 EMBL-EBI
WormBase CE01416 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA20671 Hypothetical protein T22F7.1 [Caenorhabditis elegans] ref|NP_497209.1| T22F7.1 [Caenorhabditis elegans] 0.0
blast CAE69236 Hypothetical protein CBG15279 [Caenorhabditis briggsae] 0.0
blast CAA92479 Hypothetical protein T05A1.7 [Caenorhabditis elegans] ref|NP_501698.1| T05A1.7 [Caenorhabditis elegans] 1.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 5.1E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497209.1 12 74-96,129-151,158-177,182-204,216-238,248-270,341-360,370-392,401-418,423-445,457-479,484-503
T22F7.1 12 74-96,129-151,158-177,182-204,216-238,248-270,341-360,370-392,401-418,423-445,457-479,484-503
SNAP00000006223 12 74-96,129-151,158-177,182-204,216-238,248-270,341-360,370-392,401-418,423-445,457-479,484-503
Sequence
Target Sequence 
>C. ELEGANS:183538_AT
agaaaattgccacttcatcatcgccgacaccacaaaatcgactgacaatatttgaaatga
ttcgtgaaattctgtcggatcgggagatcacacgccgaatcatcgtcgagacttttctct
ggattctcacttttatgacgtactgtgcgctgtcacttacatccacatctgtcggaaatt
cggatccgctggtgtcgtttttgttttcgggagtcgtcgagttgcctgcctacctcttca
taccgatatgcttgacgagaacaaaacgtcgcccaacccgttttctgtgccattttactg
gctcaatggcccttctcacaatgtatttcttgtcaaacgacacatcgctacacctcatca
tttggctcgttgccaagttttgcgcctcatgctgttacattttctgcttcatctacgctg
ccgagctgtttccgacgtggtgccggtcgtgctgtattggggtttgctcgacatgctgca
atattggagcaattgtggcgccgcacatttttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAAATTGCCACTTCATCATCGCC 465 73 932 Antisense
ATCACACGCCGAATCATCGTCGAGA 560 27 1018 Antisense
TATGACGTACTGTGCGCTGTCACTT 635 655 1065 Antisense
GAAATTCGGATCCGCTGGTGTCGTT 598 361 1106 Antisense
TTTTCGGGAGTCGTCGAGTTGCCTG 451 675 1135 Antisense
CCCGTTTTCTGTGCCATTTTACTGG 348 253 1208 Antisense
ATTTTACTGGCTCAATGGCCCTTCT 70 13 1223 Antisense
CTACACCTCATCATTTGGCTCGTTG 12 227 1279 Antisense
TACATTTTCTGCTTCATCTACGCTG 240 645 1327 Antisense
GTATTGGGGTTTGCTCGACATGCTG 582 451 1385 Antisense
GCAATTGTGGCGCCGCACATTTTTC 192 323 1420 Antisense
Sequence Source Affymetrix Proprietary Database