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[183529_s_at]

Description F59H5.1 /REP_DB=WormBase Gene ID /WP=CE19910 /TR=Q9TZI4 /GB=AAC67449.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 13      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 96.9
(1.2)
A 		74.2
(-0.8)
A 		48.4
(-3)
A 		118.2
(3)
A 		70
B 80.1
(-0.7)
A 		78.2
(-0.8)
A 		40.8
(-3)
A 		142.2
(3)
P 		101
C 91.5
(-0.3)
A 		52.3
(-3)
A 		64.9
(-2.1)
A 		140.1
(3)
P 		88
avr 89.5
(-0.2) 		68.2
(-1.8) 		51.4
(-3) 		133.5
(3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 222
1 2 3 4 5 6 range
A 68
(-2.3)
A 		42.5
(-3)
A 		44.5
(-2.9)
A 		46
(-2.9)
A 		144.6
(-0.2)
M 		264.6
(3)
P 		222
B 77.8
(-2.1)
A 		77.6
(-2.2)
A 		47.7
(-3)
A 		114.5
(-1.1)
A 		184.8
(0.9)
P 		259.7
(3)
P 		212
C 78.7
(-2.3)
P 		61.5
(-3)
A 		71.6
(-2.6)
A 		101
(-1.5)
A 		218.1
(3)
P 		204.9
(2.5)
P 		157
avr 74.8
(-2.4) 		60.5
(-2.8) 		54.6
(-3) 		87.2
(-2) 		182.5
(1.1) 		243.1
(3) 		189
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 35.3
(3)
A 		16.1
(-2.1)
A 		12.7
(-3)
A 		17.3
(-1.8)
A 		23
B 25.5
(1.6)
A 		19.3
(-0.2)
A 		9.4
(-3)
A 		30.3
(3)
P 		21
C 30.3
(3)
A 		15
(-3)
A 		22.6
(-0)
A 		23.6
(0.4)
P 		15
avr 30.4
(3) 		16.8
(-2.3) 		14.9
(-3) 		23.7
(0.4) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 12.5
(-2.7)
A 		15.1
(-2.4)
A 		9.7
(-3)
A 		11.7
(-2.8)
A 		43.6
(0.7)
M 		64.4
(3)
P 		55
B 27.1
(-1.9)
A 		25.8
(-2)
A 		11.6
(-3)
A 		30.3
(-1.7)
A 		75.1
(1.5)
P 		96.9
(3)
P 		85
C 29.2
(-1.7)
P 		18.8
(-2.9)
A 		18.2
(-3)
A 		20.3
(-2.8)
A 		70.6
(3)
P 		68.6
(2.8)
P 		52
avr 22.9
(-2.1) 		19.9
(-2.4) 		13.2
(-3) 		20.8
(-2.3) 		63.1
(1.7) 		76.6
(3) 		63
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183529_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4952
Sequence Type Exemplar sequence
Representative Public ID F59H5.1 NCBI
Target Description F59H5.1 /REP_DB=WormBase Gene ID /WP=CE19910 /TR=Q9TZI4 /GB=AAC67449.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061884(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061884 NCBI Caenorhabditis elegans F59H5.1 (F59H5.1) mRNA, complete cds. 11/11 None
SNAP00000038765 ENSEMBL cdna:SNAP chromosome:CEL140:II:2503849:2514626:1 9/11 None
GENEFINDER00000038770 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2504883:2514291:1 11/11 None
F59H5.1 ENSEMBL cdna:known chromosome:CEL140:II:2504879:2514847:1 gene:F59H5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2504881-2514625(+) 98.56 100.0
Public Domain and Genome References
Gene Title
Ensembl F59H5.1
Entrez Gene 173603 Entrez gene
SwissProt Q9TZI4 EMBL-EBI
WormBase CE31963 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC67449 Hypothetical protein F59H5.1 [Caenorhabditis elegans] ref|NP_494285.2| F59H5.1 [Caenorhabditis elegans] 0.0
blast AAO27841 unknown protein [Caenorhabditis elegans] 1.0E-116
blast AAO27841 unknown protein [Caenorhabditis elegans] 1.0E-104
blast AAO27841 unknown protein [Caenorhabditis elegans] 6.0E-94
blast CAA20934 Hypothetical protein F28H6.4 [Caenorhabditis elegans] ref|NP_510354.1| F28H6.4 [Caenorhabditis elegans] 1.0E-55
Sequence
Target Sequence 
>C. ELEGANS:183529_S_AT
gaccgacgaattgcctggattacaccgaatgctcaagttgctccaaaggagccagttcct
gctccaagcaagaaaaaaagcgcttttgaagcctatcaatcgagcccccaaacttttgac
acaatccagcgattttctacaaaacgacctttcttttgtggagataacttccaaattccg
ccgaaaaagccgtttctcgccgaggaagacgtgaagctggagcccatggaatatgaaaat
tctgagcaaattgtggaaaatgcggagataaaattggagcccgctgaagagttgttggac
gcagaaactgtgacagttgaagagtttttgatgaattcgtattctactgctgctccatcc
acctcgaccgcgccggcacctccaaaagctcccgtcactgctccaccagctcctcaaaaa
agtgctcagctactgggaaaaatcgattttgcgattggaaaaatgcgcgagaactttgac
agattcctacaatttgctcaacaaaattcggcggatttgacggtcgtccaacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCGACGAATTGCCTGGATTACAC 49 383 1618 Antisense
ACCGAATGCTCAAGTTGCTCCAAAG 224 89 1641 Antisense
GCTCCAAAGGAGCCAGTTCCTGCTC 490 297 1657 Antisense
GAGCCCCCAAACTTTTGACACAATC 706 381 1719 Antisense
GACACAATCCAGCGATTTTCTACAA 652 365 1735 Antisense
AAGCCGTTTCTCGCCGAGGAAGACG 461 147 1804 Antisense
GAGGAAGACGTGAAGCTGGAGCCCA 469 405 1819 Antisense
GAGCCCGCTGAAGAGTTGTTGGACG 432 385 1894 Antisense
TGAATTCGTATTCTACTGCTGCTCC 46 577 1949 Antisense
GCGCGAGAACTTTGACAGATTCCTA 81 285 2082 Antisense
GGCGGATTTGACGGTCGTCCAACGA 27 535 2127 Antisense
Sequence Source Affymetrix Proprietary Database