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[183528_at]

Description T21B10.4 /REP_DB=WormBase Gene ID /WP=CE03686 /TR=Q22625 /GB=CAA92694.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 32      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 73.7
(-3)
A 		98.6
(-1.8)
A 		198.1
(3)
A 		143
(0.3)
P 		124
B 34.2
(-3)
A 		85.6
(-0.6)
A 		150.1
(2.5)
P 		161.4
(3)
P 		127
C 56.4
(-3)
A 		102.1
(-0.9)
A 		186.9
(3)
P 		108.3
(-0.6)
P 		131
avr 54.8
(-3) 		95.4
(-1) 		178.4
(3) 		137.6
(1) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 805
1 2 3 4 5 6 range
A 59.7
(-3)
A 		55.1
(-3)
A 		105
(-2.5)
A 		231.7
(-1.3)
P 		534.5
(1.7)
P 		665.7
(3)
P 		611
B 94.8
(-2.7)
A 		54.9
(-3)
A 		183.8
(-2)
P 		255.6
(-1.5)
P 		449.6
(-0)
P 		853.7
(3)
P 		799
C 48.9
(-3)
A 		64.4
(-2.8)
A 		134
(-2.2)
A 		251.3
(-1)
P 		343.2
(-0.1)
P 		663.7
(3)
P 		615
avr 67.8
(-2.9) 		58.1
(-3) 		140.9
(-2.3) 		246.2
(-1.3) 		442.4
(0.4) 		727.7
(3) 		670
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 26.8
(-1.9)
A 		21.4
(-2.9)
A 		51.8
(3)
A 		20.9
(-3)
P 		31
B 10.9
(-3)
A 		21.1
(-0.4)
A 		34.8
(3)
P 		34.4
(2.9)
P 		24
C 18.7
(-2.9)
A 		29.3
(-1.6)
A 		65.2
(3)
P 		18.3
(-3)
P 		47
avr 18.8
(-3) 		23.9
(-2) 		50.6
(3) 		24.5
(-1.9) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 307
1 2 3 4 5 6 range
A 11
(-3)
A 		19.6
(-2.7)
A 		22.9
(-2.5)
A 		58.8
(-1.1)
P 		161.3
(3)
P 		162.1
(3)
P 		151
B 33
(-2.7)
A 		18.3
(-3)
A 		44.6
(-2.5)
P 		67.7
(-2)
P 		182.7
(0.3)
P 		318.3
(3)
P 		300
C 18.1
(-3)
A 		19.6
(-3)
A 		34.1
(-2.5)
A 		50.5
(-2)
P 		111.1
(-0.3)
P 		222.2
(3)
P 		204
avr 20.7
(-3) 		19.2
(-3) 		33.9
(-2.6) 		59
(-1.9) 		151.7
(0.7) 		234.2
(3) 		215
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183528_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3444
Sequence Type Exemplar sequence
Representative Public ID T21B10.4 NCBI
Target Description T21B10.4 /REP_DB=WormBase Gene ID /WP=CE03686 /TR=Q22625 /GB=CAA92694.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063502(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063502 NCBI Caenorhabditis elegans T21B10.4 (T21B10.4) mRNA, complete cds. 11/11 None
SNAP00000044367 ENSEMBL cdna:SNAP chromosome:CEL140:II:8940810:8942348:1 11/11 None
GENEFINDER00000044373 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8940810:8942348:1 11/11 None
T21B10.4 ENSEMBL cdna:known chromosome:CEL140:II:8940804:8942607:1 gene:T21B10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8940806-8942345(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T21B10.4
Entrez Gene 188686 Entrez gene
SwissProt Q22625 EMBL-EBI
WormBase CE03686 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92694 Hypothetical protein T21B10.4 [Caenorhabditis elegans] ref|NP_495903.1| T21B10.4 [Caenorhabditis elegans] 0.0
blast CAE59765 Hypothetical protein CBG03217 [Caenorhabditis briggsae] 1.0E-101
blast CAA88945 Hypothetical protein F13D12.5 [Caenorhabditis elegans] ref|NP_496504.1| F13D12.5 [Caenorhabditis elegans] 2.0E-78
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495903.1 1 345-367
T21B10.4 1 345-367
SNAP00000044367 1 345-367
GENEFINDER00000044373 1 345-367
Sequence
Target Sequence 
>C. ELEGANS:183528_AT
aggacggcgttattcttggagcaacaggcggtgcgtttttcacatcttatagaaagcgcg
aaggaaacatgacaaaattgtacacttctctagtaccggcaaacctgagcgcaggtttca
aatgcgaaaaattgattaaaccttcttcaatgccaaatttctacgagctcatcatcatca
agaataatgattattgcatgttgagagatggcacgaatttcgatcgtaaaaattgtgaaa
tggaacagaaagcgtacctcatttccaagcaagtttttgaagacgagccaacaaatgttg
taatgtggctgctcgttgtctgtatcatcatgtttatgattatcatgcttctctacgtct
acatctactggcttcgttctactttcgtggcaattgaagagagagctcttcccactgagt
tcgaaacggaggcatctctatttgtcgccaagcaaagaagtttccaatcagcatactacg
atcctgctctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGACGGCGTTATTCTTGGAGCAAC 670 55 749 Antisense
GCAACAGGCGGTGCGTTTTTCACAT 305 319 769 Antisense
TCTAGTACCGGCAAACCTGAGCGCA 14 615 837 Antisense
AAATTTCTACGAGCTCATCATCATC 704 115 903 Antisense
CGTACCTCATTTCCAAGCAAGTTTT 328 247 1001 Antisense
TAATGTGGCTGCTCGTTGTCTGTAT 32 637 1049 Antisense
TATGATTATCATGCTTCTCTACGTC 38 657 1083 Antisense
CTACGTCTACATCTACTGGCTTCGT 677 225 1101 Antisense
CTTCGTTCTACTTTCGTGGCAATTG 504 219 1120 Antisense
GAGGCATCTCTATTTGTCGCCAAGC 465 405 1177 Antisense
ATCAGCATACTACGATCCTGCTCTT 602 27 1215 Antisense
Sequence Source Affymetrix Proprietary Database