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[183513_at]

Description F47G6.3 /REP_DB=WormBase Gene ID /WP=CE17840 /TR=Q9TZI8 /GB=AAK18954.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 09      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 1.7
(-3)
A 		7.9
(3)
A 		1.8
(-2.9)
A 		5.1
(0.3)
A 		6
B 4.1
(-2)
A 		1.6
(-3)
A 		2.2
(-2.8)
A 		16
(3)
A 		14
C 1.5
(-3)
A 		1.9
(-2.8)
A 		7.2
(0.4)
A 		11.7
(3)
A 		10
avr 2.4
(-3) 		3.8
(-2) 		3.7
(-2.1) 		10.9
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 4
(-2)
A 		5.4
(-1.2)
A 		2.4
(-3)
A 		3.2
(-2.5)
A 		12.5
(3)
A 		6.3
(-0.7)
A 		10
B 3.3
(-1.9)
A 		5.3
(0.3)
A 		2.6
(-2.7)
A 		2.3
(-3)
A 		5.6
(0.6)
A 		7.8
(3)
A 		6
C 1.7
(-3)
A 		3.1
(-2.7)
A 		5.1
(-2.2)
A 		2.6
(-2.8)
A 		27.1
(3)
A 		1.9
(-3)
A 		25
avr 3
(-2.9) 		4.6
(-2.1) 		3.4
(-2.7) 		2.7
(-3) 		15.1
(3) 		5.3
(-1.7) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 0.6
(-2.5)
A 		1.7
(3)
A 		0.5
(-3)
A 		0.8
(-1.5)
A 		1
B 1.3
(-1.2)
A 		0.4
(-3)
A 		0.5
(-2.8)
A 		3.4
(3)
A 		3
C 0.5
(-3)
A 		0.6
(-2.7)
A 		2.5
(3)
A 		2
(1.5)
A 		2
avr 0.8
(-3) 		0.9
(-2.5) 		1.2
(-1.2) 		2.1
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.7
(-2.6)
A 		1.9
(-0.5)
A 		0.5
(-3)
A 		0.8
(-2.5)
A 		3.8
(3)
A 		1.5
(-1.2)
A 		3
B 1.2
(-1.4)
A 		1.8
(0.1)
A 		0.6
(-3)
A 		0.6
(-3)
A 		2.3
(1.4)
A 		2.9
(3)
A 		2
C 0.6
(-2.9)
A 		1
(-2.6)
A 		1.3
(-2.4)
A 		0.5
(-3)
A 		8.8
(3)
A 		0.6
(-2.9)
A 		8
avr 0.8
(-2.7) 		1.6
(-1.6) 		0.8
(-2.7) 		0.6
(-3) 		5
(3) 		1.7
(-1.5) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183513_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.148
Sequence Type Exemplar sequence
Representative Public ID F47G6.3 NCBI
Target Description F47G6.3 /REP_DB=WormBase Gene ID /WP=CE17840 /TR=Q9TZI8 /GB=AAK18954.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058457
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058457 NCBI Caenorhabditis elegans F47G6.3 (F47G6.3) mRNA, complete cds. A 
GENEFINDER00000020138 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1465633:1479861:1 None
F47G6.3 ENSEMBL cdna:known chromosome:CEL140:I:1465633:1469537:1 gene:F47G6.3 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020129 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1465632-1469537(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F47G6.3
Entrez Gene 171713 Entrez gene
SwissProt Q9TZI8 EMBL-EBI
WormBase CE34310 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK18953 Hypothetical protein F47G6.2 [Caenorhabditis elegans] ref|NP_490859.1| F47G6.2 [Caenorhabditis elegans] 0.0
blast CAE74530 Hypothetical protein CBG22284 [Caenorhabditis briggsae] 0.0
blast T33511 hypothetical protein F47G6.2 - Caenorhabditis elegans 0.0
blast AAK18954 Hypothetical protein F47G6.3 [Caenorhabditis elegans] ref|NP_490858.3| F47G6.3 [Caenorhabditis elegans] 1.0E-134
blast T33510 hypothetical protein F47G6.3 - Caenorhabditis elegans 6.0E-78
blast CAE71380 Hypothetical protein CBG18287 [Caenorhabditis briggsae] 8.0E-56
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 4.0E-97
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 1.6E-34
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490858.3 1 218-240
F47G6.3 1 218-240
GENEFINDER00000020138 1 216-238
Sequence
Target Sequence 
>C. ELEGANS:183513_AT
atacgtggagcaacagttcacagagaattttgaccctttctttgtaaaaaaaagtgtaaa
aaactttatcaaaaaatttgaaaaaaatttcaaacccggccttgaaaccggcgaattcac
ctattcaacggaccccacccgctaccgttttcgctcaactccgtcatttgaaattgtgat
ccgaattgttccatggctgctcgtatttgccatcatgcttgccactacgtcatgcttgat
ttttctgacaaaagagccttctcatgaaatggagttcgatgagatgagcgagatgacatt
gatccagtatgacagcctcttggtcgtggattccatattcgattacttgcgaaatgagac
cgttcttcgagagaataactgcccgggattggttcaaggattctttaagccggtttatga
gtcgactcttttaaacctcaaaagacagcacaaaaatgttacaaatgcgatgaaagcggt
cactcaaacgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATACGTGGAGCAACAGTTCACAGAG 315 23 654 Antisense
CAGAGAATTTTGACCCTTTCTTTGT 78 207 674 Antisense
GGCCTTGAAACCGGCGAATTCACCT 403 541 751 Antisense
GCGAATTCACCTATTCAACGGACCC 210 283 764 Antisense
GTGATCCGAATTGTTCCATGGCTGC 62 483 829 Antisense
TGTTCCATGGCTGCTCGTATTTGCC 266 563 840 Antisense
TGCTCGTATTTGCCATCATGCTTGC 641 581 851 Antisense
ATCATGCTTGCCACTACGTCATGCT 231 29 865 Antisense
GCCACTACGTCATGCTTGATTTTTC 309 279 874 Antisense
GAGCCTTCTCATGAAATGGAGTTCG 160 385 907 Antisense
GCGATGAAAGCGGTCACTCAAACGG 520 289 1120 Antisense
Sequence Source Affymetrix Proprietary Database