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[183483_at]

Description K12B6.1 /REP_DB=WormBase Gene ID /WP=CE12164 /TR=O16386 /GB=AAB65916.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 92      4grp_highest_8_8 > 06      4grp_high_8_8 > 22      6grp_highest_nonorm > 73      6grp_total > 84      4grp_total > 16      6grp_highest_sub > 04      6grp_high1_sub > 04_05      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 519
1 2 3 4 range
A 220.4
(-3)
P 		395.8
(-0.9)
P 		406.7
(-0.7)
P 		711.6
(3)
P 		491
B 199.5
(-3)
P 		302.4
(-1.8)
P 		497.9
(0.5)
P 		718.2
(3)
P 		519
C 296.1
(-3)
P 		461.7
(-0.1)
P 		330.4
(-2.4)
P 		641.8
(3)
P 		346
avr 238.7
(-3) 		386.6
(-1) 		411.7
(-0.7) 		690.5
(3) 		452

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 494
1 2 3 4 5 6 range
A 302.7
(-3)
P 		402.7
(-1.6)
P 		402.6
(-1.6)
P 		657.2
(2.1)
P 		721
(3)
P 		669.2
(2.3)
P 		418
B 227.4
(-3)
P 		328.4
(-1.5)
P 		523.5
(1.3)
P 		645.3
(3)
P 		604.1
(2.4)
P 		458.9
(0.3)
P 		418
C 328.9
(-2.3)
P 		285.8
(-3)
P 		650.1
(2.5)
P 		496.8
(0.2)
P 		680.4
(3)
P 		478.7
(-0.1)
P 		395
avr 286.3
(-3) 		339
(-2.2) 		525.4
(0.8) 		599.8
(1.9) 		668.5
(3) 		535.6
(0.9) 		382
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 80.3
(-3)
P 		85.9
(-1.7)
P 		106.3
(3)
P 		104.2
(2.5)
P 		26
B 63.6
(-3)
P 		74.7
(-2.3)
P 		115.4
(0.5)
P 		153
(3)
P 		89
C 98
(-3)
P 		132.7
(3)
P 		115.2
(-0)
P 		108.2
(-1.2)
P 		35
avr 80.6
(-3) 		97.8
(-0.5) 		112.3
(1.6) 		121.8
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 190
1 2 3 4 5 6 range
A 55.8
(-3)
P 		143.2
(0.2)
P 		87.7
(-1.8)
P 		166.7
(1.1)
P 		217.6
(3)
P 		162.9
(1)
P 		162
B 79.1
(-3)
P 		109.3
(-1.9)
P 		127.1
(-1.3)
P 		170.9
(0.3)
P 		245.5
(3)
P 		171.1
(0.3)
P 		166
C 121.9
(-1.4)
P 		87.2
(-3)
P 		165.5
(0.5)
P 		99.9
(-2.4)
P 		220.2
(3)
P 		160.2
(0.3)
P 		133
avr 85.6
(-3) 		113.2
(-1.8) 		126.8
(-1.3) 		145.8
(-0.5) 		227.8
(3) 		164.7
(0.3) 		142
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183483_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11888
Sequence Type Exemplar sequence
Representative Public ID K12B6.1 NCBI
Target Description K12B6.1 /REP_DB=WormBase Gene ID /WP=CE12164 /TR=O16386 /GB=AAB65916.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072209(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072209 NCBI Caenorhabditis elegans K12B6.1 (K12B6.1) mRNA, complete cds. 11/11 None
SNAP00000011414 ENSEMBL cdna:SNAP chromosome:CEL140:V:6306423:6310529:1 11/11 None
GENEFINDER00000011419 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6306423:6310529:1 11/11 None
K12B6.1 ENSEMBL cdna:known chromosome:CEL140:V:6306393:6310877:1 gene:K12B6.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6306392-6310529(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K12B6.1
Entrez Gene 179016 Entrez gene
SwissProt O16386 EMBL-EBI
WormBase CE12164 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65916 Hypothetical protein K12B6.1 [Caenorhabditis elegans] ref|NP_504610.1| K12B6.1 [Caenorhabditis elegans] 0.0
blast AAF99978 Hypothetical protein F56A6.1a [Caenorhabditis elegans] ref|NP_490758.1| F56A6.1a [Caenorhabditis elegans] 0.0
blast AAO38667 Paz/piwi domain-containing protein 1, isoform c [Caenorhabditis elegans] ref|NP_491323.2| PAZ/PIWI domain-containing family member (ppw-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 5.6E-52
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 5.6E-52
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:183483_AT
ttctcaccatcctcaagcaggcaaaggcgaaccgtccaaatgatagaccagatgagcttc
tgatctacttctctggagtctctgaaggacaacatgctcttgtgaatgagtattatgcaa
atcaagtcaaggccgcctgtggactgttcaacgagtcttttagaccacacatcactttga
tcctcgcctcgaaggtgcacaataccagagtctataaatccgagaatggaggaggagttt
gtaacgtcgagccaggaacagtgattgaccacacaattgtgagccctgttttgagcgaat
ggtaccatgccggctcattggctcgtcagggaaccagcaaattggtcaaatactcgctca
ttttcaataccaagaagaatgagaagttgtcagtgtacgagagactcacaaatgagctct
gctacgagatgcagattgttttccaccccaccagtcttccaattccgctccacatcgcgg
gaacttacagtgaaagaggctcccaaatgctcgctctgaagaagccgatctacaccaatg
gcgaatttaatcaggttgccaccaacgaacagctcggcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTCACCATCCTCAAGCAGGCAAA 48 691 2039 Antisense
GATGAGCTTCTGATCTACTTCTCTG 477 411 2089 Antisense
AGTCAAGGCCGCCTGTGGACTGTTC 358 61 2163 Antisense
TAGACCACACATCACTTTGATCCTC 561 651 2199 Antisense
GTGAGCCCTGTTTTGAGCGAATGGT 56 481 2317 Antisense
GAGCGAATGGTACCATGCCGGCTCA 207 385 2331 Antisense
AATACTCGCTCATTTTCAATACCAA 218 173 2387 Antisense
ACGAGATGCAGATTGTTTTCCACCC 302 93 2462 Antisense
TCCGCTCCACATCGCGGGAACTTAC 402 595 2502 Antisense
ATGCTCGCTCTGAAGAAGCCGATCT 515 41 2545 Antisense
TTGCCACCAACGAACAGCTCGGCTA 610 701 2594 Antisense
Sequence Source Affymetrix Proprietary Database