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[183462_at]

Description T22E5.3 /REP_DB=WormBase Gene ID /WP=CE04993 /TR=Q22681 /GB=AAA83613.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 80      4grp_total > 50      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 127
1 2 3 4 range
A 26.4
(-3)
A 		35.6
(-1.1)
A 		55.5
(3)
A 		36.9
(-0.8)
A 		29
B 43.7
(3)
A 		42.5
(2.8)
A 		37.3
(1.8)
A 		11.4
(-3)
A 		32
C 20.7
(-3)
A 		138.4
(3)
A 		54.7
(-1.3)
A 		27
(-2.7)
A 		118
avr 30.3
(-2.3) 		72.2
(3) 		49.2
(0.1) 		25.1
(-3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 14.2
(-2.6)
A 		9.9
(-3)
A 		59.1
(1.4)
A 		74.2
(2.8)
P 		76.4
(3)
A 		34.2
(-0.8)
A 		67
B 14.3
(-2.3)
A 		4.2
(-3)
A 		10.5
(-2.6)
A 		90.4
(3)
A 		62.5
(1.1)
A 		37.9
(-0.7)
A 		86
C 5.8
(-3)
A 		60
(0.5)
A 		14.2
(-2.5)
A 		99.6
(3)
P 		40.7
(-0.8)
A 		21.5
(-2)
A 		94
avr 11.4
(-3) 		24.7
(-2) 		27.9
(-1.7) 		88.1
(3) 		59.9
(0.8) 		31.2
(-1.5) 		77
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 9.6
(-0.2)
A 		7.7
(-1.5)
A 		14.5
(3)
A 		5.4
(-3)
A 		9
B 13.9
(3)
A 		10.5
(1.2)
A 		8.6
(0.2)
A 		2.4
(-3)
A 		12
C 6.9
(-2.6)
A 		39.8
(3)
A 		19.1
(-0.5)
A 		4.6
(-3)
A 		35
avr 10.1
(-0.6) 		19.3
(3) 		14.1
(0.9) 		4.1
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 2.6
(-3)
A 		3.5
(-2.7)
A 		12.9
(0)
A 		18.8
(1.7)
P 		23.1
(3)
A 		8.3
(-1.3)
A 		21
B 5
(-2.1)
A 		1.4
(-3)
A 		2.6
(-2.7)
A 		23.9
(2.6)
A 		25.4
(3)
A 		14.1
(0.2)
A 		24
C 2.2
(-3)
A 		18.3
(2.4)
A 		3.6
(-2.5)
A 		20
(3)
P 		13.2
(0.7)
A 		7.2
(-1.3)
A 		18
avr 3.3
(-3) 		7.7
(-1.5) 		6.4
(-1.9) 		20.9
(3) 		20.6
(2.9) 		9.9
(-0.8) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183462_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16602
Sequence Type Exemplar sequence
Representative Public ID T22E5.3 NCBI
Target Description T22E5.3 /REP_DB=WormBase Gene ID /WP=CE04993 /TR=Q22681 /GB=AAA83613.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076673(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076673 NCBI Caenorhabditis elegans T22E5.3 (T22E5.3) mRNA, complete cds. 11/11 None
SNAP00000012232 ENSEMBL cdna:SNAP chromosome:CEL140:X:6385440:6387818:1 11/11 None
GENEFINDER00000012244 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6385440:6387875:1 11/11 None
T22E5.3 ENSEMBL cdna:known chromosome:CEL140:X:6385440:6387818:1 gene:T22E5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6385439-6387818(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T22E5.3
Entrez Gene 188745 Entrez gene
SwissProt Q22681 EMBL-EBI
WormBase CE37152 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA83613 Hypothetical protein T22E5.3 [Caenorhabditis elegans] ref|NP_509074.2| T22E5.3 [Caenorhabditis elegans] 0.0
blast CAE61251 Hypothetical protein CBG05054 [Caenorhabditis briggsae] 1.0E-145
blast CAE61251 Hypothetical protein CBG05054 [Caenorhabditis briggsae] 1.0E-142
blast CAA94577 Hypothetical protein C25G4.10 [Caenorhabditis elegans] emb|CAB05615.2| Hypothetical protein C25G4.10 [Caenorhabditis elegans] ref|NP_502384.1| C25G4.10 [Caenorhabditis elegans] 3.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.1E-8
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 4.1E-5
Sequence
Target Sequence 
>C. ELEGANS:183462_AT
gattcggatttttctacctatcgatgttatatcaaggatacacgaattgagtactcagaa
actgtgattgtaaaaattgattttagtgagcaagcaccaaatagtattccaccggatacg
ttgcttgaatgctgctcaaaatataatcataaatcagattgtcagtcagtgtgctcacaa
ggtactgatgataggagatctatgttgagaccaggtgcatttcttccacctccaaaaaat
tgcgagaaagaagtggatcacctctttaaatgctccatatctcgcctaaatagtgcagca
tgctgtattcactcggaggtgtcattcttttgcttgggaatgtgtgatgttaaattcgat
attgatccgcagcgggtgaaatcctgccgaaaacatgtgacaaatgcttatcatgtctac
agtagaaaacgcgaaggggattggacggttcgtgtgacactggatcactccgtagatatt
gttgattctgatgaggtcgttctaatttctgtcaacgaatttggtccttccgaacaacat
c

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCGGATTTTTCTACCTATCGAT 31 433 949 Antisense
TATTCCACCGGATACGTTGCTTGAA 424 665 1053 Antisense
TCAGATTGTCAGTCAGTGTGCTCAC 659 623 1102 Antisense
TTGAGACCAGGTGCATTTCTTCCAC 58 703 1153 Antisense
GATCACCTCTTTAAATGCTCCATAT 489 421 1204 Antisense
GCTCCATATCTCGCCTAAATAGTGC 573 297 1220 Antisense
GTGCAGCATGCTGTATTCACTCGGA 90 481 1241 Antisense
GAGGTGTCATTCTTTTGCTTGGGAA 620 401 1264 Antisense
TCCGCAGCGGGTGAAATCCTGCCGA 548 593 1314 Antisense
GTGTGACACTGGATCACTCCGTAGA 615 487 1400 Antisense
GAATTTGGTCCTTCCGAACAACATC 473 325 1465 Antisense
Sequence Source Affymetrix Proprietary Database