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[183438_s_at]

Description F45E4.8 /REP_DB=WormBase Gene ID /WP=CE10506 /TR=Q94233 /GB=AAK29818.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 73      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 55      6grp_total > 74      4grp_total > 23      6grp_cgc > 66      6grp_high1_sub > 03_04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1294
1 2 3 4 range
A 2088.4
(3)
P 		925
(-3)
P 		1041.6
(-2.4)
P 		2066
(2.9)
P 		1163
B 2169.5
(3)
P 		876
(-3)
P 		1116.8
(-1.9)
P 		1979.9
(2.1)
P 		1294
C 2082.8
(3)
P 		880
(-3)
P 		927.8
(-2.8)
P 		1829.1
(1.7)
P 		1203
avr 2113.6
(3) 		893.7
(-3) 		1028.7
(-2.3) 		1958.3
(2.2) 		1220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1832
1 2 3 4 5 6 range
A 620.7
(-3)
P 		1341.2
(-0.6)
P 		2270.6
(2.4)
P 		2452.7
(3)
P 		1412.1
(-0.4)
P 		1698.4
(0.5)
P 		1832
B 774
(-3)
P 		1467.1
(-0.4)
P 		2368.1
(3)
P 		1712.5
(0.5)
P 		1738.5
(0.6)
P 		1951.7
(1.4)
P 		1594
C 918.2
(-3)
P 		1329.1
(-1.1)
P 		1883.6
(1.4)
P 		2182.6
(2.7)
P 		1828.8
(1.1)
P 		2242.5
(3)
P 		1324
avr 771
(-3) 		1379.1
(-0.4) 		2174.1
(3) 		2115.9
(2.8) 		1659.8
(0.8) 		1964.2
(2.1) 		1403
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 560
1 2 3 4 range
A 760.4
(3)
P 		200.8
(-3)
P 		272.3
(-2.2)
P 		302.4
(-1.9)
P 		560
B 691.8
(3)
P 		216.3
(-3)
P 		258.8
(-2.5)
P 		421.9
(-0.4)
P 		476
C 689.5
(3)
P 		253
(-3)
P 		323.4
(-2)
P 		308.4
(-2.2)
P 		437
avr 713.9
(3) 		223.4
(-3) 		284.8
(-2.2) 		344.2
(-1.5) 		491

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 636
1 2 3 4 5 6 range
A 114.4
(-3)
P 		477
(1.3)
P 		494.5
(1.5)
P 		622.1
(3)
P 		426.1
(0.7)
P 		413.5
(0.5)
P 		508
B 269.3
(-3)
P 		488.4
(-0.1)
P 		575
(1)
P 		453.6
(-0.6)
P 		706.3
(2.7)
P 		727.8
(3)
P 		459
C 340.4
(-3)
P 		405.5
(-2)
P 		479.4
(-1)
P 		438.9
(-1.6)
P 		591.8
(0.7)
P 		750.7
(3)
P 		410
avr 241.4
(-3) 		457
(0.3) 		516.3
(1.2) 		504.9
(1.1) 		574.7
(2.1) 		630.7
(3) 		389
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183438_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9559
Sequence Type Exemplar sequence
Representative Public ID F45E4.8 NCBI
Target Description F45E4.8 /REP_DB=WormBase Gene ID /WP=CE10506 /TR=Q94233 /GB=AAK29818.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068843(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068843 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-20) (nlp-20) mRNA, complete cds. 11/11 None
SNAP00000024168 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7642203:7643043:-1 11/11 None
GENEFINDER00000024178 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7642203:7643043:-1 11/11 None
F45E4.8.1 ENSEMBL cdna:known chromosome:CEL140:IV:7641717:7643055:-1 gene:F45E4.8 11/11 None
F45E4.8.2 ENSEMBL cdna:known chromosome:CEL140:IV:7642044:7643080:-1 gene:F45E4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7642208-7643049(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-20
Ensembl F45E4.8
Entrez Gene 177542 Entrez gene
SwissProt Q94233 EMBL-EBI
WormBase CE10506 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29818 Neuropeptide-like protein protein 20 [Caenorhabditis elegans] ref|NP_501244.1| Neuropeptide-Like Protein family member (nlp-20) [Caenorhabditis elegans] 2.0E-32
blast CAE61932 Hypothetical protein CBG05929 [Caenorhabditis briggsae] 1.0E-27
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501244.1 1 5-24
F45E4.8.1 1 5-24
F45E4.8.2 1 5-24
SNAP00000024168 1 5-24
GENEFINDER00000024178 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:183438_S_AT
atgcaagtcactcttcttgctcttcttctgctcatcgttcctttctttgcttttgctgca
tcgcaatattcggatgatgattctgaattgatgagcaacaacgaacgatttgctcgtgat
cttgaattgcgaaagaaatttgcattcgcatttgcaaagagaagtgcaggagatgcagat
gttgtcatcgaagctcgcagtggaccacaagctcatgaaggtgctggtatgcgctttgct
tttgcaaaaaggcgagctccaaaggagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCAAGTCACTCTTCTTGCTCTTC 14 45 13 Antisense
TTTCTTTGCTTTTGCTGCATCGCAA 697 671 54 Antisense
GCTGCATCGCAATATTCGGATGATG 340 297 67 Antisense
GATTTGCTCGTGATCTTGAATTGCG 589 427 119 Antisense
GAAAGAAATTTGCATTCGCATTTGC 379 359 143 Antisense
AGATGTTGTCATCGAAGCTCGCAGT 7 69 189 Antisense
TCGAAGCTCGCAGTGGACCACAAGC 332 599 200 Antisense
GGACCACAAGCTCATGAAGGTGCTG 215 521 214 Antisense
TGAAGGTGCTGGTATGCGCTTTGCT 359 575 228 Antisense
CTGGTATGCGCTTTGCTTTTGCAAA 267 241 236 Antisense
GCAAAAAGGCGAGCTCCAAAGGAGT 362 287 256 Antisense
Sequence Source Affymetrix Proprietary Database