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[183436_at]

Description F56B3.5 /REP_DB=WormBase Gene ID /WP=CE27172 /TR=O45106 /GB=AAK29838.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 52      6grp_cgc > 81      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 187
1 2 3 4 range
A 159.8
(3)
A 		158.9
(2.9)
A 		119
(0.6)
A 		58.8
(-3)
A 		101
B 155.1
(1.9)
A 		186.5
(3)
A 		140.3
(1.4)
A 		9.8
(-3)
A 		177
C 196.3
(3)
A 		184.4
(2.6)
A 		122.5
(0.2)
A 		37.3
(-3)
A 		159
avr 170.4
(2.7) 		176.6
(3) 		127.3
(0.9) 		35.3
(-3) 		141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 111
1 2 3 4 5 6 range
A 107.6
(2)
A 		123.7
(3)
A 		124.3
(3)
A 		25.6
(-2.8)
A 		88.3
(0.9)
A 		22.4
(-3)
A 		102
B 77.6
(-2)
A 		122.7
(2)
A 		133.8
(3)
A 		77.1
(-2.1)
A 		66.9
(-3)
A 		78.8
(-1.9)
A 		67
C 83.2
(0.5)
A 		93.1
(1.7)
A 		103.6
(3)
A 		102.1
(2.8)
A 		64.1
(-1.8)
A 		54.7
(-3)
A 		49
avr 89.5
(0.3) 		113.2
(2.4) 		120.6
(3) 		68.3
(-1.6) 		73.1
(-1.2) 		52
(-3) 		69
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 58.2
(3)
A 		34.5
(0.1)
A 		31.1
(-0.3)
A 		8.6
(-3)
A 		50
B 49.5
(3)
A 		46.1
(2.6)
A 		32.5
(0.8)
A 		2.1
(-3)
A 		47
C 65
(3)
A 		53
(1.8)
A 		42.7
(0.7)
A 		6.3
(-3)
A 		59
avr 57.6
(3) 		44.5
(1.5) 		35.4
(0.4) 		5.7
(-3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 19.8
(-0.8)
A 		44
(3)
A 		27.1
(0.4)
A 		6.5
(-2.8)
A 		26.6
(0.3)
A 		5.4
(-3)
A 		39
B 27
(-1.1)
A 		40.8
(3)
A 		32.5
(0.6)
A 		20.4
(-3)
A 		27.2
(-1)
A 		29.4
(-0.4)
A 		20
C 30.9
(3)
A 		28.4
(1.8)
A 		26.4
(0.9)
A 		20.5
(-2)
A 		20.8
(-1.8)
A 		18.3
(-3)
A 		13
avr 25.9
(-0.2) 		37.7
(3) 		28.7
(0.5) 		15.8
(-3) 		24.9
(-0.5) 		17.7
(-2.5) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183436_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11150
Sequence Type Exemplar sequence
Representative Public ID F56B3.5 NCBI
Target Description F56B3.5 /REP_DB=WormBase Gene ID /WP=CE27172 /TR=O45106 /GB=AAK29838.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067592(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067592 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-5) (ech-5) mRNA, complete cds. 11/11 None
F56B3.5 ENSEMBL cdna:known chromosome:CEL140:IV:797023:799855:1 gene:F56B3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:797029-799305(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ech-5
Ensembl F56B3.5
Entrez Gene 176908 Entrez gene
SwissProt O45106 EMBL-EBI
WormBase CE27172 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245446_AT enoyl-CoA hydratase, putative at
CANINE_2:CFA.20370.1.S1_S_AT similar to Methylglutaconyl-CoA hydratase, mitochondrial precursor (AU-specific RNA-binding enoyl-CoA hydratase) (AU-binding protein/enoyl-CoA hydratase) cfa
CANINE_2:CFA.3047.1.S1_AT similar to Methylglutaconyl-CoA hydratase, mitochondrial precursor (AU-specific RNA-binding enoyl-CoA hydratase) (AU-binding protein/enoyl-CoA hydratase) cfa
DROSOPHILA_2:1627526_AT dm
DROSGENOME1:147109_AT dm
HG-U133A_2:205052_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U133A:205052_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U133_PLUS_2:205052_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-FOCUS:205052_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HUGENEFL:X79888_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
U133_X3P:G4502326_3P_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U95AV2:37616_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U133_PLUS_2:236839_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U133B:236839_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U133_PLUS_2:240090_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U133B:240090_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U133_PLUS_2:241318_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U133B:241318_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U95B:42782_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U95D:71399_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U95E:78343_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U95E:81408_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HG-U95D:81816_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
HU35KSUBD:RC_AA490340_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
U133_X3P:236839_3P_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
U133_X3P:HS.160019.0.A1_3P_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
U133_X3P:HS.163879.0.A1_3P_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
U133_X3P:HS.262618.0.A1_3P_X_AT AU RNA binding protein/enoyl-Coenzyme A hydratase hs
MOE430A:1420776_A_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOUSE430A_2:1420776_A_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOUSE430_2:1420776_A_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOUSE430A_2:1430487_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOE430A:1430487_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOUSE430_2:1430487_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOUSE430A_2:1431124_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOUSE430_2:1431124_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOE430A:1431124_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MU11KSUBA:AA242017_S_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MG-U74AV2:96650_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MG-U74CV2:170627_I_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOE430A:1447952_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOUSE430A_2:1447952_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOUSE430_2:1447952_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MG-U74AV2:94787_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MU11KSUBA:C77140_RC_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MU11KSUBA:C77140_RC_G_AT AU RNA binding protein/enoyl-coenzyme A hydratase mm
MOUSE430_2:1444433_AT AU RNA binding protein/enoyl-coenzyme A hydratase, mRNA (cDNA clone IMAGE:4924434) mm
MOE430B:1444433_AT AU RNA binding protein/enoyl-coenzyme A hydratase, mRNA (cDNA clone IMAGE:4924434) mm
MOE430B:1445496_AT AU RNA binding protein/enoyl-coenzyme A hydratase, mRNA (cDNA clone IMAGE:4924434) mm
MOUSE430_2:1445496_AT AU RNA binding protein/enoyl-coenzyme A hydratase, mRNA (cDNA clone IMAGE:4924434) mm
MOE430B:1458436_AT AU RNA binding protein/enoyl-coenzyme A hydratase, mRNA (cDNA clone IMAGE:4924434) mm
MOUSE430_2:1458436_AT AU RNA binding protein/enoyl-coenzyme A hydratase, mRNA (cDNA clone IMAGE:4924434) mm
MU19KSUBB:TC25997_AT AU RNA binding protein/enoyl-coenzyme A hydratase, mRNA (cDNA clone IMAGE:4924434) mm
MU19KSUBB:TC26006_AT AU RNA binding protein/enoyl-coenzyme A hydratase, mRNA (cDNA clone IMAGE:4924434) mm
RG-U34A:RC_AA859688_AT AU RNA binding protein/enoyl-coenzyme A hydratase (predicted) rn
RAE230A:1372149_AT AU RNA binding protein/enoyl-coenzyme A hydratase (predicted) rn
RAT230_2:1372149_AT AU RNA binding protein/enoyl-coenzyme A hydratase (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29838 Enoyl-coa hydratase protein 5 [Caenorhabditis elegans] ref|NP_499993.1| Enoyl-CoA Hydratase family member (ech-5) [Caenorhabditis elegans] 1.0E-148
blast CAE56266 Hypothetical protein CBG23911 [Caenorhabditis briggsae] 1.0E-98
blast EAL24663 GA21314-PA [Drosophila pseudoobscura] 3.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 3.7E-58
Sequence
Target Sequence 
>C. ELEGANS:183436_AT
acacgtgtggtgattctcaactcgaaatgcgataatgtgttctgtagtggtgcggatttg
aaagaacgcaagactatgagccagcaggaggccaccagatttgtcaatgggctccgtgac
tcgtttactgatgtagaacgccttccacagccagttatcgccgcaatcgacggatttgca
ctgggaggtggattggagttggcactggcttgtgatattcgtgttgcttctcaaaaagcc
aaaatgggcctagtcgaaacaaaatgggctctaattcccggcgccggaggttctcaacgt
ctctaccgtattgtcggagtcgcaaaagccaaagagctcatctacacagctgaagtgctc
aacggagccgatgctgccaagcttggagtcgttaatcacgtcgttgaagccaatccaatc
gaaaaatcactggaaatcgcacgaaaaatcattccaagaggcccgattgctgtga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACGTGTGGTGATTCTCAACTCGA 114 111 241 Antisense
GCAGGAGGCCACCAGATTTGTCAAT 381 311 324 Antisense
CTCCGTGACTCGTTTACTGATGTAG 426 235 352 Antisense
TACTGATGTAGAACGCCTTCCACAG 47 647 366 Antisense
CCAGTTATCGCCGCAATCGACGGAT 227 273 391 Antisense
GCTTGTGATATTCGTGTTGCTTCTC 506 307 448 Antisense
CAAAATGGGCTCTAATTCCCGGCGC 224 189 500 Antisense
CTCAACGTCTCTACCGTATTGTCGG 410 227 533 Antisense
CTCAACGGAGCCGATGCTGCCAAGC 67 229 598 Antisense
ATCACGTCGTTGAAGCCAATCCAAT 14 29 635 Antisense
ATTCCAAGAGGCCCGATTGCTGTGA 417 7 691 Antisense
Sequence Source Affymetrix Proprietary Database