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[183432_s_at]

Description K10B4.3 /REP_DB=WormBase Gene ID /WP=CE25943 /TR=O17237 /GB=AAB71008.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 54      6grp_high1_10_10 > 45      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 41      6grp_total > 35      4grp_total > 62      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 263
1 2 3 4 range
A 665.6
(3)
P 		569.5
(-0.2)
P 		567.7
(-0.2)
P 		483.7
(-3)
P 		182
B 677.8
(3)
P 		576.4
(-1.9)
P 		556
(-2.9)
P 		553.7
(-3)
P 		124
C 713
(3)
P 		506.6
(-1.7)
P 		644.7
(1.4)
P 		450.3
(-3)
P 		263
avr 685.5
(3) 		550.8
(-1.3) 		589.5
(-0) 		495.9
(-3) 		190

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 462
1 2 3 4 5 6 range
A 931.9
(3)
P 		829.4
(1.5)
P 		688.7
(-0.5)
P 		712.3
(-0.1)
P 		512.4
(-3)
P 		696.8
(-0.4)
P 		420
B 884.4
(3)
P 		877.2
(2.9)
P 		771.6
(1.2)
P 		661.6
(-0.5)
P 		500.3
(-3)
P 		779.9
(1.4)
P 		384
C 786.9
(1)
P 		939.7
(3)
P 		826
(1.5)
P 		821.9
(1.5)
P 		478
(-3)
P 		784.4
(1)
P 		462
avr 867.7
(2.8) 		882.1
(3) 		762.1
(1.1) 		731.9
(0.7) 		496.9
(-3) 		753.7
(1) 		385
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 172
1 2 3 4 range
A 242.4
(3)
P 		123.6
(-1.2)
P 		148.4
(-0.3)
P 		70.8
(-3)
P 		172
B 216.1
(3)
P 		142.4
(-1.5)
P 		128.8
(-2.3)
P 		118
(-3)
P 		98
C 236
(3)
P 		145.7
(-0.4)
P 		224.7
(2.6)
P 		75.9
(-3)
P 		160
avr 231.5
(3) 		137.2
(-0.9) 		167.3
(0.3) 		88.2
(-3) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 171.8
(-2.1)
P 		295
(3)
P 		150
(-3)
P 		180.7
(-1.7)
P 		154.6
(-2.8)
P 		169.7
(-2.2)
P 		145
B 307.7
(3)
P 		292
(2.3)
P 		187.4
(-2.4)
P 		175.2
(-3)
P 		203.3
(-1.7)
P 		290.8
(2.2)
P 		133
C 291.7
(3)
P 		286.7
(2.8)
P 		210.2
(-0.6)
P 		165.3
(-2.5)
P 		154.7
(-3)
P 		262.6
(1.7)
P 		137
avr 257.1
(1.3) 		291.2
(3) 		182.5
(-2.4) 		173.7
(-2.9) 		170.9
(-3) 		241
(0.5) 		120
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183432_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5975
Sequence Type Exemplar sequence
Representative Public ID K10B4.3 NCBI
Target Description K10B4.3 /REP_DB=WormBase Gene ID /WP=CE25943 /TR=O17237 /GB=AAB71008.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061263(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061263 NCBI Caenorhabditis elegans K10B4.3 (K10B4.3) mRNA, complete cds. 11/11 None
SNAP00000039346 ENSEMBL cdna:SNAP chromosome:CEL140:II:114468:120308:-1 11/11 None
GENEFINDER00000039357 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:114468:116319:-1 11/11 None
K10B4.3 ENSEMBL cdna:known chromosome:CEL140:II:114468:121208:-1 gene:K10B4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:114467-121208(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K10B4.3
Entrez Gene 173397 Entrez gene
SwissProt O17237 EMBL-EBI
WormBase CE35995 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.14042.1.S1_AT similar to ventricular zone expressed PH domain homolog 1 cfa
DROSOPHILA_2:1626194_AT melted dm
DROSGENOME1:143969_AT melted dm
HU35KSUBB:RC_W81237_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
U133_X3P:HS.126485.2.S1_3P_A_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
U133_X3P:HS.126485.0.A1_3P_A_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U133_PLUS_2:229759_S_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U133B:229759_S_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U133_PLUS_2:229760_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U133B:229760_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U133_PLUS_2:232122_S_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U133B:232122_S_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U95C:61696_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U95E:85508_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U133_PLUS_2:234940_S_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U133B:234940_S_AT ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HU35KSUBB:RC_AA143070_AT Ventricular zone expressed PH domain homolog 1 (zebrafish) hs
HG-U95C:59502_AT Ventricular zone expressed PH domain homolog 1 (zebrafish) hs
MOUSE430_2:1432088_AT ventricular zone expressed PH domain homolog 1 (zebrafish) mm
MOE430B:1432088_AT ventricular zone expressed PH domain homolog 1 (zebrafish) mm
RG-U34B:RC_AI013299_AT similar to Veph-A (predicted) rn
RAE230A:1377437_AT similar to Veph-A (predicted) rn
RAT230_2:1377437_AT similar to Veph-A (predicted) rn
RAE230B:1381212_AT Similar to VEPH isoform A rn
RAT230_2:1381212_AT Similar to VEPH isoform A rn
Protein Similarities
Method ID Description E-Value
blast NP_493664 K10B4.3 [Caenorhabditis elegans] gb|AAB71008.3| Hypothetical protein K10B4.3 [Caenorhabditis elegans] 0.0
blast CAE62806 Hypothetical protein CBG06982 [Caenorhabditis briggsae] 0.0
blast NP_665819 VEPH isoform A [Mus musculus] dbj|BAC02920.1| Veph-A [Mus musculus] 6.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 2.6E-15
Pfam IPR001849 EMBL-EBI Pleckstrin-like 2.6E-15
Pfam IPR001849 EMBL-EBI Pleckstrin-like 2.6E-15
Sequence
Target Sequence 
>C. ELEGANS:183432_S_AT
gactcacagcagtaccaaacgcttgaacattgttggaagtgtttaccgagttctataaca
aaaagtgttccctttgatacaatgatcactgcagcttttccaaactcaaaagaccagaac
aagctgattaaggaacttgacgaagctggatttttcgcttgttttacaatggactctacg
aataatatgtggaattgcattagttgtacgaatcctgaaaaagtgaaatactttgttgaa
gaaggaggcgccgaaaaagtattggaaggacagttgaaagagaaaaaagggcgatggagg
tttttgaaacgatggaacaccaaatactttaccttgtcatcggctgcattgaattatagc
acacaacatatgccgactgattcccgagcactccttccctcaatcgatcttcgttctatc
cgatctgtcagatcgctcggtcgtggaaagaaagctagaaagagtctaagaaaggcattt
gaaattttcacagctgacaatacatcaatgattctgaaagccaaagatgaaaaaaatgcg
gaggaatggcttcattgtctacagattgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTCACAGCAGTACCAAACGCTTG 346 373 1897 Antisense
GTTGGAAGTGTTTACCGAGTTCTAT 667 399 1928 Antisense
AAGTGTTCCCTTTGATACAATGATC 66 159 1959 Antisense
TGATCACTGCAGCTTTTCCAAACTC 14 565 1979 Antisense
GACGAAGCTGGATTTTTCGCTTGTT 446 379 2035 Antisense
GAACACCAAATACTTTACCTTGTCA 561 349 2211 Antisense
TTACCTTGTCATCGGCTGCATTGAA 555 681 2225 Antisense
CAACATATGCCGACTGATTCCCGAG 382 189 2260 Antisense
TCTTCGTTCTATCCGATCTGTCAGA 595 617 2304 Antisense
GTCAGATCGCTCGGTCGTGGAAAGA 339 467 2323 Antisense
ATGGCTTCATTGTCTACAGATTGCA 44 53 2442 Antisense
Sequence Source Affymetrix Proprietary Database