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[183427_s_at]

Description Y56A3A.2 /REP_DB=WormBase Gene ID /WP=CE22573 /TR=Q9U227 /GB=CAB60513.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 68      4grp_total > 17      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 128.5
(1.2)
A 		108.2
(-3)
A 		136.9
(3)
A 		133.3
(2.2)
A 		29
B 131.5
(-1.1)
A 		162.7
(3)
A 		116.8
(-3)
A 		141.2
(0.2)
A 		46
C 88.8
(-3)
A 		114.3
(-1)
A 		113
(-1.1)
A 		164.3
(3)
A 		76
avr 116.3
(-3) 		128.4
(-0.6) 		122.2
(-1.8) 		146.3
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 65.6
(2.4)
A 		18.3
(-1.8)
A 		46.5
(0.7)
A 		4.5
(-3)
A 		34.7
(-0.3)
A 		72.5
(3)
A 		68
B 46.7
(-1.6)
A 		12.7
(-3)
A 		15.4
(-2.9)
A 		26.7
(-2.4)
A 		33.9
(-2.2)
A 		162.7
(3)
A 		150
C 32.2
(-1.7)
A 		38.4
(-1.4)
A 		85.8
(1)
A 		5.4
(-3)
A 		35.7
(-1.5)
A 		125.4
(3)
A 		120
avr 48.2
(-1) 		23.1
(-2.4) 		49.2
(-0.9) 		12.2
(-3) 		34.8
(-1.7) 		120.2
(3) 		108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 46.8
(3)
A 		23.5
(-2.1)
A 		35.8
(0.6)
A 		19.5
(-3)
A 		27
B 41.9
(3)
A 		40.2
(2.3)
A 		27.1
(-3)
A 		30.1
(-1.8)
A 		15
C 29.4
(-2.1)
A 		32.9
(-0.3)
A 		39.4
(3)
A 		27.7
(-3)
A 		12
avr 39.4
(3) 		32.2
(-0.2) 		34.1
(0.7) 		25.8
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 12.1
(1)
A 		6.5
(-1)
A 		10.1
(0.3)
A 		1.1
(-3)
A 		10.5
(0.4)
A 		17.7
(3)
A 		17
B 16.2
(-1.7)
A 		4.2
(-2.9)
A 		3.7
(-3)
A 		7.1
(-2.6)
A 		13.8
(-1.9)
A 		60.7
(3)
A 		57
C 11.9
(-1.4)
A 		11.7
(-1.4)
A 		21.9
(0.1)
A 		1.1
(-3)
A 		11.5
(-1.5)
A 		42
(3)
A 		41
avr 13.4
(-1.3) 		7.5
(-2.3) 		11.9
(-1.6) 		3.1
(-3) 		11.9
(-1.6) 		40.1
(3) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183427_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6384
Sequence Type Exemplar sequence
Representative Public ID Y56A3A.2 NCBI
Target Description Y56A3A.2 /REP_DB=WormBase Gene ID /WP=CE22573 /TR=Q9U227 /GB=CAB60513.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067136(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067136 NCBI Caenorhabditis elegans Y56A3A.2 (Y56A3A.2) mRNA, complete cds. 11/11 A 
SNAP00000035189 ENSEMBL cdna:SNAP chromosome:CEL140:III:11851383:11868779:-1 11/11 None
Y56A3A.2 ENSEMBL cdna:known chromosome:CEL140:III:11850376:11862125:-1 gene:Y56A3A.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000035217 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11851378-11861068(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y56A3A.2
Entrez Gene 176616 Entrez gene
SwissProt Q9U227 EMBL-EBI
WormBase CE31840 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:254517_AT peptidase M50 family protein / sterol-regulatory element binding protein (SREBP) site 2 protease family protein at
DROSOPHILA_2:1632678_AT dm
DROSGENOME1:147040_AT dm
CHICKEN:GGAAFFX.10490.1.S1_AT similar to Membrane-bound transcription factor site 2 protease (S2P endopeptidase) (Site-2 protease) (Sterol-regulatory element-binding proteins intramembrane protease) gga
CHICKEN:GGAAFFX.10490.2.S1_AT similar to Membrane-bound transcription factor site 2 protease (S2P endopeptidase) (Site-2 protease) (Sterol-regulatory element-binding proteins intramembrane protease) gga
CHICKEN:GGAAFFX.10490.2.S1_S_AT similar to Membrane-bound transcription factor site 2 protease (S2P endopeptidase) (Site-2 protease) (Sterol-regulatory element-binding proteins intramembrane protease) gga
HG-U133_PLUS_2:206473_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133A:206473_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133A_2:206473_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U95AV2:31416_AT membrane-bound transcription factor peptidase, site 2 hs
HU35KSUBA:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HU35KSUBB:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HU35KSUBC:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HU35KSUBD:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HUGENEFL:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HC-G110:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
U133_X3P:G7706692_3P_AT membrane-bound transcription factor peptidase, site 2 hs
U133_X3P:HS2.350970.2.S1_3P_AT membrane-bound transcription factor peptidase, site 2 hs
U133_X3P:HS.302065.0.S1_3P_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133_PLUS_2:1554604_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133A_2:216531_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133_PLUS_2:216531_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133A:216531_AT membrane-bound transcription factor peptidase, site 2 hs
MOE430B:1436883_AT membrane-bound transcription factor peptidase, site 2 mm
MOUSE430_2:1436883_AT membrane-bound transcription factor peptidase, site 2 mm
MOE430B:1437495_AT membrane-bound transcription factor peptidase, site 2 mm
MOUSE430_2:1437495_AT membrane-bound transcription factor peptidase, site 2 mm
MG-U74BV2:164110_AT membrane-bound transcription factor peptidase, site 2 mm
MG-U74CV2:132106_AT Membrane-bound transcription factor peptidase, site 2, transcript variant 1, mRNA (cDNA clone MGC:25655 IMAGE:4460515) mm
MU19KSUBA:TC21845_AT Membrane-bound transcription factor peptidase, site 2, transcript variant 1, mRNA (cDNA clone MGC:25655 IMAGE:4460515) mm
RG-U34B:RC_AI028857_AT Similar to RIKEN cDNA 9630032G22 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4222 metalloendopeptidase activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60513 Hypothetical protein Y56A3A.2 [Caenorhabditis elegans] ref|NP_499537.2| Y56A3A.2 [Caenorhabditis elegans] 0.0
blast CAE67728 Hypothetical protein CBG13303 [Caenorhabditis briggsae] 0.0
blast NP_001030179 membrane-bound transcription factor protease, site 2 [Rattus norvegicus] 1.0E-47
blast CAB60514 Hypothetical protein Y56A3A.4 [Caenorhabditis elegans] ref|NP_499538.2| TAF (TBP-associated transcription factor) family member (taf-12) [Caenorhabditis elegans] 9.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR008915 EMBL-EBI Peptidase M50 3.1E-32
Pfam IPR008915 EMBL-EBI Peptidase M50 3.2E-30
Pfam IPR003228 EMBL-EBI Transcription initiation factor TFIID 4.0E-40
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499537.2 7 4-26,90-112,206-228,241-263,283-305,552-574,589-611
Y56A3A.2 7 4-26,90-112,206-228,241-263,283-305,552-574,589-611
SNAP00000035189 4 307-329,390-412,506-528,574-596
Sequence
Target Sequence 
>C. ELEGANS:183427_S_AT
agctcgcgaatcgaaataagcctattctttttgtggggcagctcaatgagatgctcgaaa
tggtgtcaatatcgtcgttcactccgcgcttcacgttcgccagcatctcgtggctcgaac
attttgagctaaccgccaaatacctgttcacactgtctctggcacttggcctattcaatg
caatgcctgtgtatgctcttgatgggcaattcatagttcatacgcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTCGCGAATCGAAATAAGCCTAT 41 81 1514 Antisense
CCTATTCTTTTTGTGGGGCAGCTCA 64 267 1534 Antisense
ATGAGATGCTCGAAATGGTGTCAAT 462 45 1559 Antisense
TGGTGTCAATATCGTCGTTCACTCC 473 553 1574 Antisense
CAGCATCTCGTGGCTCGAACATTTT 357 203 1614 Antisense
TTGAGCTAACCGCCAAATACCTGTT 247 705 1637 Antisense
CAAATACCTGTTCACACTGTCTCTG 623 193 1650 Antisense
CTCTGGCACTTGGCCTATTCAATGC 8 233 1670 Antisense
AATGCAATGCCTGTGTATGCTCTTG 365 165 1690 Antisense
GTGTATGCTCTTGATGGGCAATTCA 155 487 1702 Antisense
TGGGCAATTCATAGTTCATACGCTA 250 555 1716 Antisense
Sequence Source Affymetrix Proprietary Database