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[183426_at]

Description Y56A3A.2 /REP_DB=WormBase Gene ID /WP=CE22573 /TR=Q9U227 /GB=CAB60513.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 21      6grp_total > 09      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 105.8
(-2.9)
P 		140.5
(3)
P 		105.9
(-2.9)
P 		105.1
(-3)
P 		35
B 94.4
(1.6)
P 		92.6
(1.2)
P 		70.4
(-3)
A 		101.8
(3)
A 		31
C 80
(-1.7)
P 		66.3
(-3)
P 		81.6
(-1.5)
P 		127.5
(3)
P 		61
avr 93.4
(-1.3) 		99.8
(0.2) 		86
(-3) 		111.5
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 67.2
(-0.7)
A 		62.5
(-1.1)
M 		55.9
(-1.6)
P 		65.1
(-0.9)
P 		115
(3)
M 		38.4
(-3)
A 		77
B 84.1
(1.6)
P 		48.5
(-1.5)
P 		100.4
(3)
P 		64.6
(-0.1)
P 		30.6
(-3)
A 		71.7
(0.5)
P 		70
C 83.6
(0.4)
P 		59.3
(-3)
P 		68
(-1.8)
A 		62.5
(-2.6)
P 		68.1
(-1.8)
A 		102
(3)
P 		43
avr 78.3
(3) 		56.8
(-3) 		74.8
(2) 		64.1
(-1) 		71.2
(1) 		70.7
(0.9) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 38.5
(3)
P 		30.5
(0.9)
P 		27.7
(0.2)
P 		15.4
(-3)
P 		23
B 30.1
(3)
P 		22.9
(-0.1)
P 		16.3
(-3)
A 		21.7
(-0.7)
A 		14
C 26.5
(1.8)
P 		19.1
(-3)
P 		28.4
(3)
P 		21.5
(-1.5)
P 		9
avr 31.7
(3) 		24.2
(-0.7) 		24.1
(-0.7) 		19.5
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 12.4
(-2.3)
A 		22.2
(0)
M 		12.2
(-2.3)
P 		16.5
(-1.3)
P 		34.7
(3)
M 		9.3
(-3)
A 		25
B 29.3
(3)
P 		16.1
(-1.7)
P 		24.4
(1.3)
P 		17.1
(-1.3)
P 		12.4
(-3)
A 		26.8
(2.1)
P 		17
C 31
(2.1)
P 		18.1
(-1.5)
P 		17.3
(-1.7)
A 		12.6
(-3)
P 		22.1
(-0.3)
A 		34.1
(3)
P 		22
avr 24.2
(3) 		18.8
(-0.7) 		18
(-1.2) 		15.4
(-3) 		23.1
(2.3) 		23.4
(2.5) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183426_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6384
Sequence Type Exemplar sequence
Representative Public ID Y56A3A.2 NCBI
Target Description Y56A3A.2 /REP_DB=WormBase Gene ID /WP=CE22573 /TR=Q9U227 /GB=CAB60513.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067136(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067136 NCBI Caenorhabditis elegans Y56A3A.2 (Y56A3A.2) mRNA, complete cds. 11/11 A 
SNAP00000035189 ENSEMBL cdna:SNAP chromosome:CEL140:III:11851383:11868779:-1 11/11 None
GENEFINDER00000035217 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11852726:11857125:-1 11/11 None
Y56A3A.2 ENSEMBL cdna:known chromosome:CEL140:III:11850376:11862125:-1 gene:Y56A3A.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11851378-11861068(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y56A3A.2
Entrez Gene 176616 Entrez gene
SwissProt Q9U227 EMBL-EBI
WormBase CE31840 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:254517_AT peptidase M50 family protein / sterol-regulatory element binding protein (SREBP) site 2 protease family protein at
DROSOPHILA_2:1632678_AT dm
DROSGENOME1:147040_AT dm
CHICKEN:GGAAFFX.10490.1.S1_AT similar to Membrane-bound transcription factor site 2 protease (S2P endopeptidase) (Site-2 protease) (Sterol-regulatory element-binding proteins intramembrane protease) gga
CHICKEN:GGAAFFX.10490.2.S1_AT similar to Membrane-bound transcription factor site 2 protease (S2P endopeptidase) (Site-2 protease) (Sterol-regulatory element-binding proteins intramembrane protease) gga
CHICKEN:GGAAFFX.10490.2.S1_S_AT similar to Membrane-bound transcription factor site 2 protease (S2P endopeptidase) (Site-2 protease) (Sterol-regulatory element-binding proteins intramembrane protease) gga
HG-U133_PLUS_2:206473_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133A:206473_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133A_2:206473_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U95AV2:31416_AT membrane-bound transcription factor peptidase, site 2 hs
HU35KSUBA:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HU35KSUBB:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HU35KSUBC:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HU35KSUBD:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HUGENEFL:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
HC-G110:HUM_ALU_AT membrane-bound transcription factor peptidase, site 2 hs
U133_X3P:G7706692_3P_AT membrane-bound transcription factor peptidase, site 2 hs
U133_X3P:HS2.350970.2.S1_3P_AT membrane-bound transcription factor peptidase, site 2 hs
U133_X3P:HS.302065.0.S1_3P_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133_PLUS_2:1554604_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133A_2:216531_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133_PLUS_2:216531_AT membrane-bound transcription factor peptidase, site 2 hs
HG-U133A:216531_AT membrane-bound transcription factor peptidase, site 2 hs
MOE430B:1436883_AT membrane-bound transcription factor peptidase, site 2 mm
MOUSE430_2:1436883_AT membrane-bound transcription factor peptidase, site 2 mm
MOE430B:1437495_AT membrane-bound transcription factor peptidase, site 2 mm
MOUSE430_2:1437495_AT membrane-bound transcription factor peptidase, site 2 mm
MG-U74BV2:164110_AT membrane-bound transcription factor peptidase, site 2 mm
MG-U74CV2:132106_AT Membrane-bound transcription factor peptidase, site 2, transcript variant 1, mRNA (cDNA clone MGC:25655 IMAGE:4460515) mm
MU19KSUBA:TC21845_AT Membrane-bound transcription factor peptidase, site 2, transcript variant 1, mRNA (cDNA clone MGC:25655 IMAGE:4460515) mm
RG-U34B:RC_AI028857_AT Similar to RIKEN cDNA 9630032G22 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4222 metalloendopeptidase activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60513 Hypothetical protein Y56A3A.2 [Caenorhabditis elegans] ref|NP_499537.2| Y56A3A.2 [Caenorhabditis elegans] 0.0
blast CAE67728 Hypothetical protein CBG13303 [Caenorhabditis briggsae] 0.0
blast CAE67728 Hypothetical protein CBG13303 [Caenorhabditis briggsae] 1.0E-160
blast NP_001030179 membrane-bound transcription factor protease, site 2 [Rattus norvegicus] 1.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR008915 EMBL-EBI Peptidase M50 3.1E-32
Pfam IPR008915 EMBL-EBI Peptidase M50 3.2E-30
Pfam IPR003228 EMBL-EBI Transcription initiation factor TFIID 4.0E-40
Pfam IPR008915 EMBL-EBI Peptidase M50 3.2E-17
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499537.2 7 4-26,90-112,206-228,241-263,283-305,552-574,589-611
Y56A3A.2 7 4-26,90-112,206-228,241-263,283-305,552-574,589-611
SNAP00000035189 4 307-329,390-412,506-528,574-596
GENEFINDER00000035217 3 91-113,123-145,166-188
Sequence
Target Sequence 
>C. ELEGANS:183426_AT
gtgacgagtttaacgtgaccaccgctcacgtctgtttccagcgagaagagaaagccagca
agttgtcggtcgacacacagacacgagctccacagctcaatgctcttctcgggttagaag
gcggcggcggcggccgagatgatgccttaccttacggtggtgttgcacagcctgaagaga
agaagatcacaaagtatagctgccttcatgcgagatatgtggttcagcaggcgctctgta
acgccagtgagccgtgtaaaacggaggaaaccccagcagcacatcaggagaagatctgtg
tctatccggcactacacaatggaacgcggctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGACGAGTTTAACGTGACCACCGC 483 483 1172 Antisense
CTCACGTCTGTTTCCAGCGAGAAGA 211 227 1196 Antisense
GAAAGCCAGCAAGTTGTCGGTCGAC 363 359 1221 Antisense
GTCGGTCGACACACAGACACGAGCT 175 471 1236 Antisense
CGAGATGATGCCTTACCTTACGGTG 268 243 1306 Antisense
CTTACGGTGGTGTTGCACAGCCTGA 491 221 1322 Antisense
GTATAGCTGCCTTCATGCGAGATAT 375 453 1365 Antisense
TGGTTCAGCAGGCGCTCTGTAACGC 377 551 1391 Antisense
AAACCCCAGCAGCACATCAGGAGAA 695 123 1439 Antisense
AGAAGATCTGTGTCTATCCGGCACT 691 73 1460 Antisense
GGCACTACACAATGGAACGCGGCTT 438 535 1479 Antisense
Sequence Source Affymetrix Proprietary Database