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[183425_at]

Description F35C8.5 /REP_DB=WormBase Gene ID /WP=CE04497 /TR=Q20027 /GB=AAA81710.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 45      6grp_total > 73      4grp_total > 49      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 711
1 2 3 4 range
A 1544.7
(-3)
P 		2240.5
(2.9)
P 		2256.1
(3)
P 		1550.8
(-2.9)
P 		711
B 1716.5
(-3)
P 		2126.4
(2.8)
P 		2143.9
(3)
P 		1943.8
(0.2)
P 		427
C 1634.1
(-3)
P 		2032.1
(1.8)
P 		2128.4
(3)
P 		1771.3
(-1.3)
P 		494
avr 1631.8
(-3) 		2133
(2.5) 		2176.1
(3) 		1755.3
(-1.6) 		544

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1683
1 2 3 4 5 6 range
A 1527.9
(-2.9)
P 		1517.9
(-3)
P 		2113.5
(1.5)
P 		2179.4
(1.9)
P 		2320.4
(3)
P 		1726.9
(-1.4)
P 		803
B 1258.3
(-3)
P 		1515.6
(-2)
P 		2206.5
(0.5)
P 		2867.3
(3)
P 		2118.1
(0.2)
P 		1801.9
(-1)
P 		1609
C 1184.5
(-3)
P 		1630.6
(-1)
P 		2224.1
(1.6)
P 		2533.6
(3)
P 		1884.2
(0.1)
P 		1981.2
(0.5)
P 		1349
avr 1323.6
(-3) 		1554.7
(-1.8) 		2181.4
(1.3) 		2526.8
(3) 		2107.6
(0.9) 		1836.7
(-0.4) 		1203
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 515
1 2 3 4 range
A 562.4
(2.5)
P 		486.3
(1.3)
P 		589.8
(3)
P 		227
(-3)
P 		363
B 547.4
(3)
P 		525.2
(2)
P 		496.8
(0.7)
P 		414.2
(-3)
P 		133
C 540.9
(0.3)
P 		584.2
(0.9)
P 		741.8
(3)
P 		298.7
(-3)
P 		443
avr 550.2
(1.8) 		531.9
(1.4) 		609.5
(3) 		313.3
(-3) 		296

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 579
1 2 3 4 5 6 range
A 281.7
(-3)
P 		539.8
(0.7)
P 		460.3
(-0.4)
P 		552.8
(0.9)
P 		700.2
(3)
P 		420.4
(-1)
P 		419
B 437.8
(-3)
P 		504.5
(-2.1)
P 		535.8
(-1.6)
P 		759.4
(1.6)
P 		860.6
(3)
P 		671.9
(0.3)
P 		423
C 439.1
(-3)
P 		497.5
(-1.4)
P 		566.1
(0.4)
P 		509.5
(-1.1)
P 		609.7
(1.6)
P 		663.2
(3)
P 		224
avr 386.2
(-3) 		513.9
(-0.7) 		520.7
(-0.6) 		607.2
(0.9) 		723.5
(3) 		585.2
(0.5) 		337
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183425_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16515
Sequence Type Exemplar sequence
Representative Public ID F35C8.5 NCBI
Target Description F35C8.5 /REP_DB=WormBase Gene ID /WP=CE04497 /TR=Q20027 /GB=AAA81710.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076511(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076511 NCBI Caenorhabditis elegans F35C8.5 (F35C8.5) mRNA, complete cds. 11/11 None
SNAP00000043829 ENSEMBL cdna:SNAP chromosome:CEL140:X:5365780:5367538:1 11/11 None
GENEFINDER00000043849 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5365780:5367538:1 11/11 None
F35C8.5 ENSEMBL cdna:known chromosome:CEL140:X:5365780:5367538:1 gene:F35C8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5365780-5367539(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35C8.5
Entrez Gene 185268 Entrez gene
SwissProt Q20027 EMBL-EBI
WormBase CE04497 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1598286_AT similar to cholesterol 25-hydroxylase cfa
CANINE_2:CFA.16947.1.A1_AT similar to cholesterol 25-hydroxylase cfa
CANINE_2:CFAAFFX.24077.1.S1_AT similar to cholesterol 25-hydroxylase cfa
CHICKEN:GGA.10928.1.S1_AT cholesterol 25-hydroxylase gga
HG-U95AV2:32363_AT cholesterol 25-hydroxylase hs
HG-U133_PLUS_2:206932_AT cholesterol 25-hydroxylase hs
HG-U133A:206932_AT cholesterol 25-hydroxylase hs
HG-FOCUS:206932_AT cholesterol 25-hydroxylase hs
HG-U133A_2:206932_AT cholesterol 25-hydroxylase hs
HU35KSUBC:RC_N51873_AT cholesterol 25-hydroxylase hs
U133_X3P:G4502498_3P_AT cholesterol 25-hydroxylase hs
HG-U95B:45019_AT cholesterol 25-hydroxylase hs
MOUSE430_2:1449227_AT cholesterol 25-hydroxylase mm
MOE430A:1449227_AT cholesterol 25-hydroxylase mm
MOUSE430A_2:1449227_AT cholesterol 25-hydroxylase mm
MG-U74AV2:104509_AT cholesterol 25-hydroxylase mm
RAE230B:1380063_AT cholesterol 25-hydroxylase (predicted) rn
RAT230_2:1380063_AT cholesterol 25-hydroxylase (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81710 Hypothetical protein F35C8.5 [Caenorhabditis elegans] ref|NP_508912.1| F35C8.5 [Caenorhabditis elegans] 1.0E-177
blast CAE68743 Hypothetical protein CBG14675 [Caenorhabditis briggsae] 1.0E-167
blast CAG05263 unnamed protein product [Tetraodon nigroviridis] 2.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR006088 EMBL-EBI Sterol desaturase 7.9E-88
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508912.1 4 54-73,98-115,130-152,187-209
F35C8.5 4 54-73,98-115,130-152,187-209
SNAP00000043829 4 54-73,98-115,130-152,187-209
GENEFINDER00000043849 4 54-73,98-115,130-152,187-209
Sequence
Target Sequence 
>C. ELEGANS:183425_AT
tcagctcctgtggatttacccgatggcacttgtccagttaatttgggtacctgatacaga
gcttcctatcctggcaccaacagtttttgaaatgctttcacagctggctatatttttcct
ggcctttgatttcacatatttctggttccattatatcaatcacaaggtaaaatggctcta
ccgctggtgtcactctgttcatcatatgtactcatccccattcgcagcatctgcacaaca
tcttcacccatttgagctgttctttgttggaacattcatcaccactatcccatggatctt
cccaactcactgcttgacctactggatttggttctttatcgcccagagcgtctcttatga
ggttcacattggatacgacttcccatttgctcttcatcgcattttctggttctactcggg
tgctccggctcatgacatgcaccatctgagaccactgacatgtttccaaccgtggttcaa
ctatctcgacagactcatgggttaccatattacctacgccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAGCTCCTGTGGATTTACCCGATG 691 623 309 Antisense
TTACCCGATGGCACTTGTCCAGTTA 297 681 324 Antisense
TTTTCCTGGCCTTTGATTTCACATA 431 675 422 Antisense
TAAAATGGCTCTACCGCTGGTGTCA 61 639 476 Antisense
GGTGTCACTCTGTTCATCATATGTA 127 499 494 Antisense
ACATCTTCACCCATTTGAGCTGTTC 614 105 546 Antisense
CTCACTGCTTGACCTACTGGATTTG 565 227 614 Antisense
ATTGGATACGACTTCCCATTTGCTC 500 3 676 Antisense
TCTTCATCGCATTTTCTGGTTCTAC 169 617 699 Antisense
TGAGACCACTGACATGTTTCCAACC 666 567 755 Antisense
TGGGTTACCATATTACCTACGCCGA 174 551 806 Antisense
Sequence Source Affymetrix Proprietary Database