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[183424_at]

Description M03F8.2 /REP_DB=WormBase Gene ID /WP=CE12374 /TR=O16377 /GB=AAB65910.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 49      6grp_high1_10_10 > 93      4grp_highest_8_8 > 01      4grp_high_8_8 > 53      6grp_highest_nonorm > 90      6grp_total > 80      4grp_total > 50      6grp_highest_sub > 04_05_06      6grp_cgc > 77      6grp_high1_sub > 04      6grp_cgc_sub > 04_05_06      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 150
1 2 3 4 range
A 146
(1.3)
P 		181.8
(3)
P 		134.4
(0.8)
P 		55.2
(-3)
P 		127
B 113.7
(-1.8)
P 		183.5
(3)
P 		136
(-0.2)
P 		95.6
(-3)
P 		88
C 110.6
(-3)
P 		145.7
(-0.8)
P 		205.5
(3)
P 		150.7
(-0.5)
P 		95
avr 123.4
(-1) 		170.3
(3) 		158.6
(2) 		100.5
(-3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 161.3
(1.9)
P 		157.8
(1.5)
P 		170.1
(3)
P 		120.9
(-3)
P 		168.3
(2.8)
P 		138.2
(-0.9)
P 		49
B 125
(-1.4)
P 		130.9
(-0.9)
P 		106.1
(-3)
P 		175.9
(3)
P 		117.7
(-2)
P 		167.6
(2.3)
P 		70
C 123.9
(-1.7)
P 		148
(-0.1)
P 		104.4
(-3)
P 		194.5
(3)
P 		141.7
(-0.5)
P 		166.6
(1.1)
P 		90
avr 136.7
(-1.4) 		145.6
(0) 		126.9
(-3) 		163.8
(3) 		142.6
(-0.4) 		157.5
(2) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 53.2
(3)
P 		39.5
(1.2)
P 		35.1
(0.6)
P 		8.1
(-3)
P 		45
B 36.3
(0.8)
P 		45.3
(3)
P 		31.5
(-0.3)
P 		20.4
(-3)
P 		25
C 36.6
(-1.5)
P 		41.9
(-0.9)
P 		71.6
(3)
P 		25.4
(-3)
P 		46
avr 42
(2.1) 		42.2
(2.2) 		46.1
(3) 		18
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 29.7
(-3)
P 		56.1
(3)
P 		37
(-1.3)
P 		30.7
(-2.8)
P 		50.8
(1.8)
P 		33.6
(-2.1)
P 		26
B 43.5
(-0.1)
P 		43.6
(-0.1)
P 		25.8
(-3)
P 		46.6
(0.4)
P 		47.8
(0.6)
P 		62.5
(3)
P 		37
C 45.9
(1)
P 		45.1
(0.8)
P 		26.6
(-3)
P 		39.1
(-0.4)
P 		45.8
(0.9)
P 		55.8
(3)
P 		29
avr 39.7
(-0.1) 		48.3
(2.3) 		29.8
(-3) 		38.8
(-0.4) 		48.1
(2.3) 		50.6
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183424_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12190
Sequence Type Exemplar sequence
Representative Public ID M03F8.2 NCBI
Target Description M03F8.2 /REP_DB=WormBase Gene ID /WP=CE12374 /TR=O16377 /GB=AAB65910.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171463(10), NM_001028889(10), NM_171464(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171463 NCBI Caenorhabditis elegans PAPS (adenosine 3'-phosphate 5'-phosphosulfate) Transporter family member (pst-1) (pst-1) mRNA, complete cds. 10/11 None
NM_001028889 NCBI Caenorhabditis elegans PAPS (adenosine 3'-phosphate 5'-phosphosulfate) Transporter family member (pst-1) (pst-1) mRNA, complete cds. 10/11 None
NM_171464 NCBI Caenorhabditis elegans PAPS (adenosine 3'-phosphate 5'-phosphosulfate) Transporter family member (pst-1) (pst-1) mRNA, complete cds. 10/11 None
SNAP00000043839 ENSEMBL cdna:SNAP chromosome:CEL140:V:5947330:5950966:1 10/11 None
GENEFINDER00000043861 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5946083:5952550:1 10/11 None
M03F8.2a ENSEMBL cdna:known chromosome:CEL140:V:5946068:5953984:1 gene:M03F8.2 11/11 None
M03F8.2b ENSEMBL cdna:known chromosome:CEL140:V:5947016:5950966:1 gene:M03F8.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5946082-5953544(+) 100.0 100.0
Public Domain and Genome References
Ensembl M03F8.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741545 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) Transporter family member (pst-1) [Caenorhabditis elegans] gb|AAX55686.1| Paps (adenosine 3'-phosphate 5'-phosphosulfate) transporter protein 1, isoform a [Caenorhabditis elegans] sp|Q8MXJ9|PST1_CAEEL Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (PAPS transporter 1) 0.0
blast NP_741546 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) Transporter family member (pst-1) [Caenorhabditis elegans] gb|AAX55687.1| Paps (adenosine 3'-phosphate 5'-phosphosulfate) transporter protein 1, isoform b [Caenorhabditis elegans] 0.0
blast CAE64539 Hypothetical protein CBG09282 [Caenorhabditis briggsae] 0.0
blast NP_001024060 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) Transporter family member (pst-1) [Caenorhabditis elegans] gb|AAX55688.1| Paps (adenosine 3'-phosphate 5'-phosphosulfate) transporter protein 1, isoform c [Caenorhabditis elegans] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741545.2 9 24-46,102-124,144-166,233-252,262-284,305-327,342-360,365-387,391-408
NP_001024060.1 7 39-61,117-139,159-181,248-270,280-302,322-344,364-386
NP_741546.3 9 39-61,117-139,159-181,248-267,277-299,320-342,357-375,380-402,406-423
M03F8.2a 7 24-46,102-124,144-166,233-255,265-287,307-329,349-371
M03F8.2b 7 39-61,117-139,159-181,248-270,280-302,322-344,364-386
SNAP00000043839 7 39-61,117-139,159-181,248-270,280-302,322-344,364-386
GENEFINDER00000043861 9 24-46,102-124,144-166,233-252,262-284,305-327,342-360,365-387,391-408
Sequence
Target Sequence 
>C. ELEGANS:183424_AT
gtttgttcttgttgagctcatcatcgaaaggagccggatcgactattacatacacatcgt
tttccggaatgattctcatggctggatatcttctcttcgatgcattcacgctgaattggc
agaaagctttatttgatacaaaaccaaaagtttccaaatatcagatgatgttcggtgtca
acttcttctcagctatattatgtgcagtttctctaatagaacaaggaactctgtggtctt
ctatcaaatttggagccgaacacgttgatttctctcgtgatgtcttccttctttctctat
ctggagccatcggacaaatcttcatctattcaactattgagagatttggcccgattgtat
ttgcagtgatcatgactattcggcagatattcataagaaatactctgataagagccgagg
accacagaggagttgagatggctccacctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTGTTCTTGTTGAGCTCATCATC 17 453 755 Antisense
GAGCCGGATCGACTATTACATACAC 620 385 785 Antisense
TTCTCATGGCTGGATATCTTCTCTT 640 691 827 Antisense
TTCTCTTCGATGCATTCACGCTGAA 130 693 845 Antisense
TGATGTTCGGTGTCAACTTCTTCTC 263 565 920 Antisense
GGAACTCTGTGGTCTTCTATCAAAT 574 529 979 Antisense
GGAGCCGAACACGTTGATTTCTCTC 599 521 1006 Antisense
CTTCTTTCTCTATCTGGAGCCATCG 270 221 1042 Antisense
GGAGCCATCGGACAAATCTTCATCT 78 523 1057 Antisense
GATTTGGCCCGATTGTATTTGCAGT 612 425 1097 Antisense
GAGGAGTTGAGATGGCTCCACCTCC 127 405 1181 Antisense
Sequence Source Affymetrix Proprietary Database