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[183421_at]

Description F25E5.5 /REP_DB=WormBase Gene ID /WP=CE07139 /TR=O76651 /GB=AAG24076.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 53      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 90      6grp_total > 40      4grp_total > 31      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 161.6
(3)
P 		118.9
(-1.5)
P 		104.7
(-3)
P 		111.5
(-2.3)
P 		57
B 147.7
(1.1)
P 		103.7
(-3)
P 		152.1
(1.5)
P 		168.3
(3)
P 		65
C 177.2
(3)
P 		150.3
(-2.5)
P 		147.8
(-3)
P 		157.2
(-1.1)
P 		29
avr 162.2
(3) 		124.3
(-3) 		134.9
(-1.3) 		145.7
(0.4) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 172
(-1)
P 		224.6
(0.6)
P 		281.4
(2.3)
P 		306.4
(3)
P 		103.9
(-3)
P 		134.5
(-2.1)
P 		203
B 162.4
(-0.7)
P 		204.2
(2.1)
P 		218
(3)
P 		174.1
(0.1)
P 		164
(-0.6)
P 		127.6
(-3)
P 		90
C 143.4
(-1.3)
P 		196.6
(2.9)
P 		197.3
(3)
P 		194.5
(2.8)
P 		121.7
(-3)
P 		190.8
(2.5)
P 		76
avr 159.3
(-1.3) 		208.5
(1.6) 		232.2
(3) 		225
(2.6) 		129.9
(-3) 		151
(-1.8) 		102
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 58.9
(3)
P 		25.8
(-1.7)
P 		27.4
(-1.4)
P 		16.3
(-3)
P 		43
B 47.1
(3)
P 		25.6
(-3)
P 		35.2
(-0.3)
P 		35.9
(-0.1)
P 		22
C 58.6
(3)
P 		43.2
(0.1)
P 		51.5
(1.7)
P 		26.5
(-3)
P 		32
avr 54.9
(3) 		31.5
(-1.9) 		38
(-0.5) 		26.2
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 31.7
(-3)
P 		79.9
(3)
P 		61.3
(0.7)
P 		77.7
(2.7)
P 		31.3
(-3)
P 		32.8
(-2.8)
P 		49
B 56.5
(-0.2)
P 		68
(3)
P 		52.9
(-1.1)
P 		46.1
(-3)
P 		66.6
(2.6)
P 		47.6
(-2.6)
P 		22
C 53.2
(0.4)
P 		60
(2.1)
P 		50.2
(-0.3)
P 		39.1
(-3)
P 		39.4
(-2.9)
P 		63.9
(3)
P 		25
avr 47.1
(-2.7) 		69.3
(3) 		54.8
(-0.7) 		54.3
(-0.8) 		45.8
(-3) 		48.1
(-2.4) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183421_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11604
Sequence Type Exemplar sequence
Representative Public ID F25E5.5 NCBI
Target Description F25E5.5 /REP_DB=WormBase Gene ID /WP=CE07139 /TR=O76651 /GB=AAG24076.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072512(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072512 NCBI Caenorhabditis elegans F25E5.5 (F25E5.5) mRNA, complete cds. 11/11 None
SNAP00000010677 ENSEMBL cdna:SNAP chromosome:CEL140:V:7449868:7451476:1 10/11 None
GENEFINDER00000010688 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7449868:7451476:1 11/11 None
F25E5.5 ENSEMBL cdna:known chromosome:CEL140:V:7449865:7451476:1 gene:F25E5.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7449868-7451477(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25E5.5
Entrez Gene 3565479 Entrez gene
SwissProt O76651 EMBL-EBI
WormBase CE07139 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG24076 Hypothetical protein F25E5.5 [Caenorhabditis elegans] ref|NP_504913.1| F25E5.5 [Caenorhabditis elegans] 0.0
blast NP_504159 Y45G5AM.3 [Caenorhabditis elegans] gb|AAG23365.1| Hypothetical protein Y45G5AM.3 [Caenorhabditis elegans] 1.0E-131
blast NP_504159 Y45G5AM.3 [Caenorhabditis elegans] gb|AAG23365.1| Hypothetical protein Y45G5AM.3 [Caenorhabditis elegans] 1.0E-129
blast NP_504640 T05H4.15a [Caenorhabditis elegans] gb|AAB66020.1| Hypothetical protein T05H4.15a [Caenorhabditis elegans] 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 5.0E-7
Sequence
Target Sequence 
>C. ELEGANS:183421_AT
gtcaaatcgagtcactacctttatgtaagaacgttcgtgttggatcagatgacattaatg
tctatcgttggtggcttcgaagagtacccgaagacctgaacagccttaaacttatcagac
ttgaggaagatagaaaagtttttacaataccaacggatttgcttgagctacctcacgtaa
tgaatgtttcatcgttctacttttactgcagagctgctttcacagatgaacaatttctca
ggctgaaagcacaatcgatgagttttgactgtatctatattacggatgggtgtatcaatg
agtttttgaagagatggataaacgggaaaggcattgatggtttccggcaagtgtttttgt
ggtcgaccagagaaagaagtcaaattgaacttttgaggggaattgaagctcggccatggg
atgaggcatttgaggaagaagcacgtggcttctgtggagattttgagagagtttgtggtc
gtggtcagtgcttccagatatcaagccgtattgatccatatgaaagcttgacgctcagta
tttctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAAATCGAGTCACTACCTTTATG 633 463 614 Antisense
ATTAATGTCTATCGTTGGTGGCTTC 614 11 667 Antisense
GTACCCGAAGACCTGAACAGCCTTA 670 453 697 Antisense
AACGGATTTGCTTGAGCTACCTCAC 622 143 765 Antisense
GTTTCATCGTTCTACTTTTACTGCA 150 451 799 Antisense
GGCATTGATGGTTTCCGGCAAGTGT 453 537 943 Antisense
GGCAAGTGTTTTTGTGGTCGACCAG 643 533 959 Antisense
AGGGGAATTGAAGCTCGGCCATGGG 519 55 1009 Antisense
AAGCACGTGGCTTCTGTGGAGATTT 370 151 1052 Antisense
GTCGTGGTCAGTGCTTCCAGATATC 32 473 1091 Antisense
AAAGCTTGACGCTCAGTATTTCTGA 165 125 1136 Antisense
Sequence Source Affymetrix Proprietary Database