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[183419_at]

Description B0024.13 /REP_DB=WormBase Gene ID /WP=CE05157 /TR=Q17428 /GB=CAA94885.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 77      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 84      6grp_total > 78      4grp_total > 61      6grp_highest_sub > Cu      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 448
1 2 3 4 range
A 695.6
(3)
P 		614.9
(1.4)
P 		630.7
(1.7)
P 		397.6
(-3)
P 		298
B 527.6
(3)
P 		503.1
(1.9)
P 		503.3
(1.9)
P 		392.7
(-3)
P 		135
C 657.4
(3)
P 		559.5
(1.6)
P 		653.6
(2.9)
P 		247.6
(-3)
P 		410
avr 626.9
(3) 		559.2
(1.6) 		595.9
(2.3) 		346
(-3) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 444
1 2 3 4 5 6 range
A 609.2
(-0.9)
P 		548.5
(-1.9)
P 		532.7
(-2.2)
P 		486.9
(-3)
P 		668.7
(0.2)
P 		830.4
(3)
P 		344
B 504.5
(-2.5)
P 		593.8
(-1.3)
P 		465.7
(-3)
P 		555
(-1.8)
P 		720.6
(0.4)
P 		909.2
(3)
P 		444
C 556.6
(-2.5)
P 		635
(-1)
P 		531.5
(-3)
P 		552.8
(-2.6)
P 		535.1
(-2.9)
P 		843.8
(3)
P 		312
avr 556.8
(-2.2) 		592.4
(-1.6) 		510
(-3) 		531.6
(-2.6) 		641.5
(-0.8) 		861.1
(3) 		351
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 212
1 2 3 4 range
A 253.3
(3)
P 		133.5
(-0.7)
P 		164.9
(0.3)
P 		58.2
(-3)
P 		195
B 168.2
(3)
P 		124.3
(-0.1)
P 		116.6
(-0.7)
P 		83.7
(-3)
P 		85
C 217.6
(2.7)
P 		160.9
(0.8)
P 		227.8
(3)
P 		41.7
(-3)
P 		186
avr 213
(3) 		139.6
(0.1) 		169.8
(1.3) 		61.2
(-3) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 228
1 2 3 4 5 6 range
A 112.3
(-3)
P 		195.1
(2.5)
P 		116
(-2.8)
P 		123.5
(-2.3)
P 		201.8
(3)
P 		202.2
(3)
P 		90
B 175.5
(-1.3)
P 		197.6
(-0.8)
P 		113.1
(-3)
P 		147
(-2.1)
P 		292.8
(1.8)
P 		339
(3)
P 		226
C 206.3
(0.3)
P 		193.8
(-0.1)
P 		135.3
(-2.2)
P 		111.2
(-3)
P 		173.1
(-0.8)
P 		282.5
(3)
P 		171
avr 164.7
(-1.3) 		195.5
(-0.1) 		121.5
(-3) 		127.2
(-2.8) 		222.6
(1) 		274.6
(3) 		153
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183419_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12174
Sequence Type Exemplar sequence
Representative Public ID B0024.13 NCBI
Target Description B0024.13 /REP_DB=WormBase Gene ID /WP=CE05157 /TR=Q17428 /GB=CAA94885.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026170(11), NM_001026171(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026170 NCBI Caenorhabditis elegans B0024.13a (B0024.13) mRNA, complete cds. 11/11 None
NM_001026171 NCBI Caenorhabditis elegans B0024.13b (B0024.13) mRNA, complete cds. 11/11 None
SNAP00000027139 ENSEMBL cdna:SNAP chromosome:CEL140:V:10323298:10324795:1 11/11 None
GENEFINDER00000027153 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10323298:10324795:1 11/11 None
B0024.13a ENSEMBL cdna:known chromosome:CEL140:V:10323298:10324795:1 gene:B0024.13 11/11 None
B0024.13b ENSEMBL cdna:known chromosome:CEL140:V:10323298:10324795:1 gene:B0024.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10323297-10324795(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0024.13
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAF31498 Hypothetical protein B0024.13b [Caenorhabditis elegans] ref|NP_001021342.1| B0024.13b [Caenorhabditis elegans] 0.0
blast CAA94885 Hypothetical protein B0024.13a [Caenorhabditis elegans] ref|NP_001021341.1| B0024.13a [Caenorhabditis elegans] 1.0E-171
blast CAA94885 Hypothetical protein B0024.13a [Caenorhabditis elegans] ref|NP_001021341.1| B0024.13a [Caenorhabditis elegans] 1.0E-170
blast CAF31498 Hypothetical protein B0024.13b [Caenorhabditis elegans] ref|NP_001021342.1| B0024.13b [Caenorhabditis elegans] 1.0E-165
Protein Domains
Database ID Description E-Value
Pfam IPR001104 EMBL-EBI 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal 1.0E-126
Pfam IPR001104 EMBL-EBI 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021341.1 7 15-37,72-91,111-133,153-170,185-207,228-250,265-287
NP_001021342.1 6 15-37,71-93,145-167,187-204,272-294,301-323
B0024.13a 7 15-37,72-91,111-133,153-170,185-207,228-250,265-287
B0024.13b 6 15-37,71-93,145-167,187-204,272-294,301-323
SNAP00000027139 7 15-37,72-91,111-133,153-170,185-207,228-250,265-287
GENEFINDER00000027153 6 15-37,71-93,145-167,187-204,272-294,301-323
Sequence
Target Sequence 
>C. ELEGANS:183419_AT
caagcacatcaatcttggctctcctgctcataagccttcacgtagcacgacgtctctatg
aaacaatatttgtcagtgtttactcggacagtcgtatgaacttgtttcactatgcagttg
ggatagtccactacatcattctgccaatttcaattatgtgtgaaacacagggagttgctt
ccaaattacctcagcttcacgtgtcaattgatgatatttctctcacccaatgggctggag
ctgtccttttttggatttgcaattggaaacaacatcagctagccgaacaaatagcaaata
caagaaaaggtcctagaggactaattcgcaattacgcctatggtatctgctttggtggtt
ggttcaatctcgtctcatgtcctcactttctattcgagatatgcatctacttgtcacttt
ttcttgtaattcctgatgcctacgtgtatag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGCACATCAATCTTGGCTCTCCT 536 185 467 Antisense
TCCTGCTCATAAGCCTTCACGTAGC 675 593 488 Antisense
GGATAGTCCACTACATCATTCTGCC 673 509 587 Antisense
GTTGCTTCCAAATTACCTCAGCTTC 91 439 640 Antisense
TGATGATATTTCTCTCACCCAATGG 111 567 675 Antisense
CAATGGGCTGGAGCTGTCCTTTTTT 321 181 694 Antisense
ACGCCTATGGTATCTGCTTTGGTGG 159 91 800 Antisense
TTGGTGGTTGGTTCAATCTCGTCTC 626 703 818 Antisense
CTCATGTCCTCACTTTCTATTCGAG 64 229 840 Antisense
GATATGCATCTACTTGTCACTTTTT 569 425 864 Antisense
TAATTCCTGATGCCTACGTGTATAG 254 633 893 Antisense
Sequence Source Affymetrix Proprietary Database