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[183418_at]

Description K12B6.3 /REP_DB=WormBase Gene ID /WP=CE12168 /TR=O16380 /GB=AAB65918.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 71      4grp_total > 48      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 14.4
(-2.6)
A 		60.7
(1.2)
P 		82
(3)
P 		9.4
(-3)
A 		73
B 45.9
(-0.9)
A 		41.9
(-1.1)
A 		121.1
(3)
P 		5
(-3)
A 		116
C 28.7
(-2.9)
A 		75.1
(-0.4)
A 		137.6
(3)
P 		27.7
(-3)
A 		110
avr 29.7
(-2.1) 		59.2
(-0.3) 		113.6
(3) 		14
(-3) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 38
(-3)
A 		71
(-1.6)
P 		156.8
(1.9)
P 		182.4
(3)
P 		73.1
(-1.5)
P 		64.3
(-1.9)
A 		144
B 5.4
(-3)
A 		124.3
(1.3)
P 		169.9
(3)
P 		159.3
(2.6)
P 		103.5
(0.6)
P 		59.8
(-1)
A 		165
C 33.5
(-3)
A 		119.1
(-0.3)
P 		150
(0.7)
P 		223.2
(3)
P 		74
(-1.7)
P 		86.7
(-1.3)
A 		190
avr 25.6
(-3) 		104.8
(-0.1) 		158.9
(1.9) 		188.3
(3) 		83.5
(-0.9) 		70.3
(-1.4) 		163
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 5.2
(-1.9)
A 		13.2
(0.5)
P 		21.4
(3)
P 		1.4
(-3)
A 		20
B 14.6
(0)
A 		10.4
(-0.9)
A 		28.1
(3)
P 		1.1
(-3)
A 		27
C 9.5
(-2.3)
A 		21.6
(-0.7)
A 		48
(3)
P 		4.7
(-3)
A 		43
avr 9.8
(-1.5) 		15.1
(-0.5) 		32.5
(3) 		2.4
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 7
(-3)
A 		25.2
(-0.2)
P 		34.1
(1.1)
P 		46.3
(3)
P 		22.1
(-0.7)
P 		15.6
(-1.7)
A 		39
B 1.9
(-3)
A 		41.4
(2.9)
P 		41.3
(2.9)
P 		42.2
(3)
P 		42
(3)
P 		22.3
(0)
A 		40
C 12.4
(-3)
A 		36.4
(1.4)
P 		38.2
(1.8)
P 		44.9
(3)
P 		23.9
(-0.9)
P 		29
(0.1)
A 		33
avr 7.1
(-3) 		34.3
(1.4) 		37.9
(1.9) 		44.5
(3) 		29.3
(0.6) 		22.3
(-0.6) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183418_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12172
Sequence Type Exemplar sequence
Representative Public ID K12B6.3 NCBI
Target Description K12B6.3 /REP_DB=WormBase Gene ID /WP=CE12168 /TR=O16380 /GB=AAB65918.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072203(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072203 NCBI Caenorhabditis elegans K12B6.3 (K12B6.3) mRNA, complete cds. 11/11 None
SNAP00000011408 ENSEMBL cdna:SNAP chromosome:CEL140:V:6281599:6282740:1 11/11 None
GENEFINDER00000011423 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6281599:6282740:1 11/11 None
K12B6.3 ENSEMBL cdna:known chromosome:CEL140:V:6281599:6282740:1 gene:K12B6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6281598-6282740(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K12B6.3
Entrez Gene 187316 Entrez gene
SwissProt O16380 EMBL-EBI
WormBase CE12168 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
16042 lipid catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16298 lipase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB65918 Hypothetical protein K12B6.3 [Caenorhabditis elegans] ref|NP_504604.1| K12B6.3 [Caenorhabditis elegans] 0.0
blast CAE71857 Hypothetical protein CBG18901 [Caenorhabditis briggsae] 1.0E-150
blast CAE66341 Hypothetical protein CBG11593 [Caenorhabditis briggsae] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR002918 EMBL-EBI Lipase, class 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504604.1 1 13-35
K12B6.3 1 13-35
SNAP00000011408 1 13-35
GENEFINDER00000011423 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:183418_AT
gctggtgctaactatggtctttgtggatgcgcaggaacagtcggcttgtttgctcccact
tgcaacaaaaagaatggcttttggcctggagattcgtgtggaggctatacatcatcattc
tgtggttattcatctactccctgcaatggaatttccaaatactcgaaccttctcaatgat
atgaacaactcgaacaaaaaggaagcctcttacattttcagtacttggagtaaagccgac
gatctcattggtaacagtgatattgtatggggccgaccaacttctctcatcccactcagc
gacggaaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGGTGCTAACTATGGTCTTTGTG 323 295 640 Antisense
GCAGGAACAGTCGGCTTGTTTGCTC 377 311 670 Antisense
TTGTTTGCTCCCACTTGCAACAAAA 550 701 685 Antisense
TGGCTTTTGGCCTGGAGATTCGTGT 70 537 714 Antisense
GTGGAGGCTATACATCATCATTCTG 111 491 737 Antisense
ATTCTGTGGTTATTCATCTACTCCC 130 11 756 Antisense
ATTTCCAAATACTCGAACCTTCTCA 517 15 790 Antisense
GAAGCCTCTTACATTTTCAGTACTT 51 345 841 Antisense
AAAGCCGACGATCTCATTGGTAACA 143 123 871 Antisense
TATTGTATGGGGCCGACCAACTTCT 304 665 900 Antisense
CTCATCCCACTCAGCGACGGAAAGA 546 229 925 Antisense
Sequence Source Affymetrix Proprietary Database