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[183413_at]

Description Y50E8A.7 /REP_DB=WormBase Gene ID /WP=CE24406 /TR=Q9U276 /GB=CAB60584.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 27      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 6
(-2.8)
A 		60.4
(2.1)
A 		3.6
(-3)
A 		70.9
(3)
A 		67
B 24.1
(-2.6)
A 		35.6
(-0.1)
A 		22.4
(-3)
A 		49.7
(3)
A 		27
C 4.6
(-3)
A 		32.8
(3)
A 		11.5
(-1.5)
A 		7.5
(-2.4)
A 		28
avr 11.6
(-3) 		42.9
(3) 		12.5
(-2.8) 		42.7
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 182
1 2 3 4 5 6 range
A 10.7
(-2.7)
A 		2.1
(-3)
A 		7.2
(-2.8)
A 		83.2
(-0.3)
A 		183.7
(3)
M 		55.8
(-1.2)
A 		182
B 39.2
(-1)
A 		17
(-2.4)
A 		6.5
(-3)
A 		103.9
(3)
A 		104.5
(3)
P 		88.3
(2)
P 		98
C 2.7
(-2.9)
A 		26.9
(-1.8)
A 		1.6
(-3)
A 		132.8
(3)
A 		128.7
(2.8)
P 		71.6
(0.2)
A 		131
avr 17.5
(-2.4) 		15.3
(-2.5) 		5.1
(-3) 		106.6
(1.5) 		139
(3) 		71.9
(-0) 		134
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 2.2
(-2.4)
A 		13.1
(3)
A 		0.9
(-3)
A 		10.4
(1.7)
A 		12
B 7.7
(-0.2)
A 		8.8
(1)
A 		5.2
(-3)
A 		10.6
(3)
A 		5
C 1.5
(-2.9)
A 		9.4
(3)
A 		4
(-1)
A 		1.3
(-3)
A 		8
avr 3.8
(-2.7) 		10.4
(3) 		3.4
(-3) 		7.4
(0.4) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 2
(-2.9)
A 		0.7
(-3)
A 		1.6
(-2.9)
A 		21.1
(-0.8)
A 		55.4
(3)
M 		13.6
(-1.6)
A 		55
B 13.6
(-1.2)
A 		5.6
(-2.4)
A 		1.6
(-3)
A 		27.5
(0.8)
A 		42.4
(3)
P 		32.9
(1.6)
P 		41
C 1
(-2.9)
A 		8.2
(-1.9)
A 		0.4
(-3)
A 		26.7
(0.8)
A 		41.7
(3)
P 		24
(0.4)
A 		41
avr 5.5
(-2.4) 		4.8
(-2.5) 		1.2
(-3) 		25.1
(0.2) 		46.5
(3) 		23.5
(-0) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183413_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12574
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.7 NCBI
Target Description Y50E8A.7 /REP_DB=WormBase Gene ID /WP=CE24406 /TR=Q9U276 /GB=CAB60584.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074240(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074240 NCBI Caenorhabditis elegans Y50E8A.7 (Y50E8A.7) mRNA, complete cds. 11/11 None
GENEFINDER00000009981 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14744166:14751294:-1 11/11 None
Y50E8A.7 ENSEMBL cdna:known chromosome:CEL140:V:14748273:14751294:-1 gene:Y50E8A.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000008983 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14748272-14751294(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50E8A.7
Entrez Gene 190108 Entrez gene
SwissProt Q9U276 EMBL-EBI
WormBase CE24406 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
16789 carboxylic ester hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60584 Hypothetical protein Y50E8A.7 [Caenorhabditis elegans] ref|NP_506641.1| Y50E8A.7 [Caenorhabditis elegans] 0.0
blast T31611 hypothetical protein Y50E8A.g - Caenorhabditis elegans 0.0
blast CAE60845 Hypothetical protein CBG04554 [Caenorhabditis briggsae] 1.0E-180
blast CAE60845 Hypothetical protein CBG04554 [Caenorhabditis briggsae] 1.0E-177
Protein Domains
Database ID Description E-Value
Pfam IPR006693 EMBL-EBI Ab-hydrolase associated lipase region 2.8E-20
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 16.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 5.7
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Pfam IPR000684 EMBL-EBI Eukaryotic RNA polymerase II heptapeptide repeat 18.0
Sequence
Target Sequence 
>C. ELEGANS:183413_AT
gaagccattgatgccatttacattactggaagaaaattatttgcaagcattgattcagtt
cgcacttgatggaaaattcgggattcttcctgtagagataccaagagcaattgcttcgaa
attcgccgacttttgttcttctaaattttttacatttttgtgttcggctggattcaaagt
tgcagctggtattgagacactcggccaggtcaatgattcaagaattccaatcattctcag
tcattttccatctgcgacatctacattgaatctgttacattggatgcagatatttaagta
tcatgaattaagaagattagatctgggaactgcaagaaatttgattgcttacgggcagaa
ggatgcgccaagactggaaattggaaacattatagcccagactatcttgtattttagcaa
agatgatcaaatcaccgatgaagttgatgtacgggaaattataatgaaacagatgggtcc
tggacttattgagagttatgatctagatcatttcactcattttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCCATTGATGCCATTTACATTA 459 345 606 Antisense
GGGATTCTTCCTGTAGAGATACCAA 203 495 685 Antisense
GAGCAATTGCTTCGAAATTCGCCGA 62 391 710 Antisense
TCGAAATTCGCCGACTTTTGTTCTT 494 601 721 Antisense
TACATTTTTGTGTTCGGCTGGATTC 157 645 756 Antisense
TGCAGCTGGTATTGAGACACTCGGC 640 569 786 Antisense
GACACTCGGCCAGGTCAATGATTCA 511 367 801 Antisense
AATCATTCTCAGTCATTTTCCATCT 323 171 834 Antisense
ATTTTCCATCTGCGACATCTACATT 227 15 848 Antisense
AGCCCAGACTATCTTGTATTTTAGC 405 77 999 Antisense
GATCTAGATCATTTCACTCATTTTG 549 419 1105 Antisense
Sequence Source Affymetrix Proprietary Database