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[183401_at]

Description C25H3.7 /REP_DB=WormBase Gene ID /WP=CE02505 /TR=Q18182 /GB=AAK31452.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 40      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 23      6grp_total > 51      4grp_total > 52      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1038
1 2 3 4 range
A 1791.7
(3)
P 		1773.5
(2.9)
P 		1631.8
(2)
P 		804.2
(-3)
P 		988
B 1787.2
(2.8)
P 		1817.3
(3)
P 		1702.3
(2.2)
P 		996
(-3)
P 		821
C 1782.5
(2.6)
P 		1841.8
(3)
P 		1671
(2)
P 		854.2
(-3)
P 		988
avr 1787.1
(2.8) 		1810.9
(3) 		1668.4
(2.1) 		884.8
(-3) 		926

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1463
1 2 3 4 5 6 range
A 1866.1
(-0.9)
P 		2187.9
(0.5)
P 		2770.5
(3)
P 		2573.1
(2.2)
P 		1366
(-3)
P 		1507
(-2.4)
P 		1405
B 1902.5
(-1)
P 		1962.2
(-0.7)
P 		2828.8
(3)
P 		2070
(-0.3)
P 		1480.1
(-2.8)
P 		1432.5
(-3)
P 		1396
C 1928.7
(-0.2)
P 		1994.5
(0.1)
P 		2489.9
(2.8)
P 		2522.4
(3)
P 		1644
(-1.8)
P 		1422.1
(-3)
P 		1100
avr 1899.1
(-0.9) 		2048.2
(-0.1) 		2696.4
(3) 		2388.5
(1.5) 		1496.7
(-2.8) 		1453.9
(-3) 		1243
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 535
1 2 3 4 range
A 652.3
(3)
P 		385
(0)
P 		426.6
(0.5)
P 		117.7
(-3)
P 		535
B 569.9
(3)
P 		448.8
(1)
P 		394.5
(0.1)
P 		212.2
(-3)
P 		358
C 590
(3)
P 		529.5
(2.2)
P 		582.4
(2.9)
P 		144
(-3)
P 		446
avr 604.1
(3) 		454.4
(1) 		467.8
(1.2) 		158
(-3) 		446

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 434
1 2 3 4 5 6 range
A 344
(-3)
P 		778.1
(3)
P 		603.4
(0.6)
P 		652.6
(1.3)
P 		412.2
(-2.1)
P 		366.9
(-2.7)
P 		434
B 662
(2)
P 		653.1
(1.7)
P 		686.9
(3)
P 		548.3
(-2.4)
P 		601.3
(-0.4)
P 		534.2
(-3)
P 		153
C 714.9
(3)
P 		608.6
(0.3)
P 		633.7
(1)
P 		507.2
(-2.2)
P 		532
(-1.6)
P 		476
(-3)
P 		239
avr 573.6
(0.1) 		679.9
(3) 		641.3
(2) 		569.4
(-0) 		515.2
(-1.5) 		459
(-3) 		221
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183401_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5768
Sequence Type Exemplar sequence
Representative Public ID C25H3.7 NCBI
Target Description C25H3.7 /REP_DB=WormBase Gene ID /WP=CE02505 /TR=Q18182 /GB=AAK31452.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170999(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170999 NCBI Caenorhabditis elegans C25H3.7b (C25H3.7) mRNA, complete cds. 11/11 None
SNAP00000016803 ENSEMBL cdna:SNAP chromosome:CEL140:II:5666876:5668898:-1 11/11 None
GENEFINDER00000016823 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5666876:5668898:-1 11/11 None
C25H3.7b ENSEMBL cdna:known chromosome:CEL140:II:5666746:5668899:-1 gene:C25H3.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171000 7/11 Cross Hyb Matching Probes None
C25H3.7a 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173961 2 1 NM_170999 183401_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5666872-5668895(-) 100.0 100.0
Public Domain and Genome References
Ensembl C25H3.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM81113 Hypothetical protein C25H3.7b [Caenorhabditis elegans] ref|NP_740998.1| C25H3.7b [Caenorhabditis elegans] 1.0E-157
blast CAE59167 Hypothetical protein CBG02473 [Caenorhabditis briggsae] 1.0E-148
blast AAK31452 Hypothetical protein C25H3.7a [Caenorhabditis elegans] ref|NP_740999.1| C25H3.7a [Caenorhabditis elegans] 1.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 9.0E-7
Sequence
Target Sequence 
>C. ELEGANS:183401_AT
ggaacgtggcttacacttcgtcaatctccacgaggacttctaccattcattgagctcaat
ggacaacaaatttcggattctcaagttatcgtatggaaacttcagaaacactttgatctc
gatgacaagcttgaaggatcggatagaggaacggctcgagctgtggaaagaatgattgat
ttgtcaactaattatgctcttctcgtcgacaaaactgtgaacaatgctcatcttcttctt
gcaagacaagtcagcaatcttccacttccatctttcctcaccaactaccttgccaaagga
ttcagtcagactgctcgtaaacgtgtgaatggagttctcggaaaactggatgtggccgag
caaaaagaactgctcagacgagatattcgagcaatcgatgatattttgggcgacaaaaag
ttcttgtttggtgacagaatcacttcggttgattgttccgtattcggacaaatcggtgcc
gtattctacctcccgtatcgtcaacaaatcagcgatcttcttgaagacgattttccacgt
gtacgtgcctactgtgaccgtatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACGTGGCTTACACTTCGTCAAT 659 529 241 Antisense
GTCAATCTCCACGAGGACTTCTACC 577 461 260 Antisense
ACTTCTACCATTCATTGAGCTCAAT 460 103 276 Antisense
TAATTATGCTCTTCTCGTCGACAAA 124 635 429 Antisense
GAACAATGCTCATCTTCTTCTTGCA 198 353 459 Antisense
AGACAAGTCAGCAATCTTCCACTTC 175 73 484 Antisense
TCAGTCAGACTGCTCGTAAACGTGT 349 623 542 Antisense
ATCACTTCGGTTGATTGTTCCGTAT 687 27 679 Antisense
ACAAATCGGTGCCGTATTCTACCTC 316 111 708 Antisense
GAAGACGATTTTCCACGTGTACGTG 386 339 763 Antisense
TGTACGTGCCTACTGTGACCGTATC 117 545 780 Antisense
Sequence Source Affymetrix Proprietary Database