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[183388_at]

Description F23B12.4 /REP_DB=WormBase Gene ID /WP=CE09596 /TR=Q19751 /GB=CAB01166.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 09      6grp_high1_10_10 > 90      4grp_high_8_8 > 52      6grp_highest_nonorm > 63      6grp_total > 92      4grp_total > 59      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1020
1 2 3 4 range
A 746.5
(0.6)
P 		1104.7
(3)
P 		1009.6
(2.4)
P 		196.7
(-3)
M 		908
B 797.3
(0.7)
P 		992.1
(1.8)
P 		1193
(3)
P 		173.4
(-3)
P 		1020
C 731.1
(1.1)
P 		980.1
(3)
P 		949.5
(2.8)
P 		183.2
(-3)
A 		797
avr 758.3
(1) 		1025.6
(2.8) 		1050.7
(3) 		184.4
(-3) 		866

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 478
1 2 3 4 5 6 range
A 462.1
(-2.3)
P 		790.5
(2.1)
P 		445.4
(-2.5)
P 		555.4
(-1)
P 		854.3
(3)
P 		408.3
(-3)
P 		446
B 536.9
(-1)
P 		702.5
(1.5)
P 		573.8
(-0.4)
P 		566.3
(-0.5)
P 		798.2
(3)
P 		405.4
(-3)
P 		393
C 493.3
(-1.3)
P 		603.9
(0.4)
P 		592
(0.2)
P 		554.6
(-0.4)
P 		782.3
(3)
P 		376.7
(-3)
P 		406
avr 497.4
(-1.5) 		699
(1.4) 		537.1
(-1) 		558.8
(-0.7) 		811.6
(3) 		396.8
(-3) 		415
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 302
1 2 3 4 range
A 271.8
(3)
P 		239.8
(2.2)
P 		263.9
(2.8)
P 		28.8
(-3)
M 		243
B 254.3
(2.4)
P 		245
(2.2)
P 		276.5
(3)
P 		37
(-3)
P 		240
C 242
(1.2)
P 		281.8
(2)
P 		330.9
(3)
P 		30.9
(-3)
A 		300
avr 256
(2.2) 		255.5
(2.2) 		290.4
(3) 		32.2
(-3) 		258

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 239
1 2 3 4 5 6 range
A 85.2
(-3)
P 		281.1
(3)
P 		97
(-2.6)
P 		140.9
(-1.3)
P 		257.8
(2.3)
P 		99.4
(-2.6)
P 		196
B 186.8
(-1.5)
P 		233.8
(0.1)
P 		139.3
(-3)
P 		150
(-2.7)
P 		324.3
(3)
P 		151.2
(-2.6)
P 		185
C 182.8
(0)
P 		184.3
(0.1)
P 		150.7
(-1.3)
P 		111.5
(-3)
P 		253.2
(3)
P 		126.1
(-2.4)
P 		142
avr 151.6
(-2) 		233.1
(1.2) 		129
(-2.9) 		134.1
(-2.7) 		278.4
(3) 		125.6
(-3) 		153
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183388_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12590
Sequence Type Exemplar sequence
Representative Public ID F23B12.4 NCBI
Target Description F23B12.4 /REP_DB=WormBase Gene ID /WP=CE09596 /TR=Q19751 /GB=CAB01166.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074177(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074177 NCBI Caenorhabditis elegans F23B12.4 (F23B12.4) mRNA, complete cds. 11/11 None
GENEFINDER00000025810 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14447253:14447790:1 11/11 None
F23B12.4 ENSEMBL cdna:known chromosome:CEL140:V:14444913:14447923:1 gene:F23B12.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000025804 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14447252-14447790(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F23B12.4
Entrez Gene 179944 Entrez gene
SwissProt Q19751 EMBL-EBI
WormBase CE09596 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01166 Hypothetical protein F23B12.4 [Caenorhabditis elegans] ref|NP_506578.1| F23B12.4 [Caenorhabditis elegans] 7.0E-50
blast CAE60898 Hypothetical protein CBG04613 [Caenorhabditis briggsae] 1.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.6E-12
Sequence
Target Sequence 
>C. ELEGANS:183388_AT
ggcttccaacgtgggatcttctggagatgctcaagtgactgctgcaccgacaacacaagc
tacattcacacaacaaccaacgttccaacaaacattccgaccaattcctgcgcgtccacg
acagcgaattgttgctcaacagccacaatctccatttaatcgtcaaaatgtgttcatttc
aagaagacgtggacagagtaacagttttgctgctcagcggcaaaatgtattccagcagcc
acagccggctttcggtggcggacaatgcgctgatcgtgatcctcaaggaagatgcagtca
gtggcgtcagttctgtgcttcaccatcccatcagcaatacttaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCTTCCAACGTGGGATCTTCTGGA 109 537 15 Antisense
GAGATGCTCAAGTGACTGCTGCACC 142 397 38 Antisense
CACCGACAACACAAGCTACATTCAC 245 201 59 Antisense
TTCACACAACAACCAACGTTCCAAC 405 685 79 Antisense
CAACAAACATTCCGACCAATTCCTG 461 189 100 Antisense
TGCGCGTCCACGACAGCGAATTGTT 678 583 123 Antisense
ACAGCCACAATCTCCATTTAATCGT 247 109 153 Antisense
AGTAACAGTTTTGCTGCTCAGCGGC 94 63 211 Antisense
GGACAATGCGCTGATCGTGATCCTC 283 527 274 Antisense
GCAGTCAGTGGCGTCAGTTCTGTGC 341 311 308 Antisense
TCACCATCCCATCAGCAATACTTAA 662 625 334 Antisense
Sequence Source Affymetrix Proprietary Database