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[183387_at]

Description C25H3.3 /REP_DB=WormBase Gene ID /WP=CE02501 /TR=Q18187 /GB=AAK31450.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 63      6grp_highest_nonorm > 62      6grp_total > 61      4grp_total > 59      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 311
1 2 3 4 range
A 332.8
(1.1)
P 		431
(3)
P 		353.5
(1.5)
P 		119.8
(-3)
A 		311
B 349.7
(2.8)
P 		333.7
(2)
P 		353
(3)
P 		232
(-3)
A 		121
C 286.4
(0.4)
P 		399.1
(3)
P 		390.3
(2.8)
P 		143.2
(-3)
A 		256
avr 323
(1.3) 		387.9
(3) 		365.6
(2.4) 		165
(-3) 		223

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 309
1 2 3 4 5 6 range
A 393.3
(-2.1)
P 		460.3
(-0.8)
P 		539.3
(0.8)
P 		654.9
(3)
P 		346
(-3)
P 		481.5
(-0.4)
P 		309
B 346.7
(-3)
P 		450.4
(0.8)
P 		431.7
(0.1)
P 		510
(3)
P 		432.3
(0.1)
P 		427.7
(-0)
P 		163
C 414.9
(-2.4)
P 		497.2
(0.5)
P 		569.9
(3)
P 		457.1
(-0.9)
P 		398.1
(-3)
P 		460.9
(-0.8)
P 		172
avr 385
(-3) 		469.3
(0.2) 		513.6
(2) 		540.7
(3) 		392.1
(-2.7) 		456.7
(-0.2) 		156
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 121.2
(3)
P 		93.6
(1.4)
P 		92.4
(1.3)
P 		17.5
(-3)
A 		104
B 111.5
(3)
P 		82.4
(0.2)
P 		81.8
(0.1)
P 		49.4
(-3)
A 		62
C 94.8
(0.8)
P 		114.7
(1.9)
P 		136
(3)
P 		24.1
(-3)
A 		112
avr 109.2
(3) 		96.9
(2.1) 		103.4
(2.6) 		30.3
(-3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 72.5
(-3)
P 		163.7
(2.8)
P 		117.4
(-0.1)
P 		166.1
(3)
P 		104.4
(-1)
P 		117.2
(-0.1)
P 		94
B 120.6
(-1.7)
P 		149.9
(0.8)
P 		104.8
(-3)
P 		135.1
(-0.4)
P 		175.6
(3)
P 		159.5
(1.6)
P 		71
C 153.8
(3)
P 		151.7
(2.8)
P 		145.1
(2.1)
P 		91.9
(-3)
P 		128.8
(0.5)
P 		154.3
(3)
P 		62
avr 115.6
(-3) 		155.1
(3) 		122.4
(-2) 		131
(-0.7) 		136.3
(0.1) 		143.7
(1.3) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183387_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4757
Sequence Type Exemplar sequence
Representative Public ID C25H3.3 NCBI
Target Description C25H3.3 /REP_DB=WormBase Gene ID /WP=CE02501 /TR=Q18187 /GB=AAK31450.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062714, NM_182268
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182268 NCBI Caenorhabditis elegans C25H3.3 (C25H3.3) mRNA, complete cds. None
NM_062714 NCBI Caenorhabditis elegans C25H3.14 (C25H3.14) mRNA, complete cds. None
SNAP00000016810 ENSEMBL cdna:SNAP chromosome:CEL140:II:5694174:5694707:1 None
SNAP00000016811 ENSEMBL cdna:SNAP chromosome:CEL140:II:5695362:5696225:1 None
C25H3.3 ENSEMBL cdna:known chromosome:CEL140:II:5694174:5694707:1 gene:C25H3.3 None
C25H3.14.1 ENSEMBL cdna:known chromosome:CEL140:II:5695351:5696578:1 gene:C25H3.14 None
C25H3.14.2 ENSEMBL cdna:known chromosome:CEL140:II:5695351:5696579:1 gene:C25H3.14 None
C25H3.14.3 ENSEMBL cdna:known chromosome:CEL140:II:5695348:5696307:1 gene:C25H3.14 None
GENEFINDER00000016815 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5695362:5696225:1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000016821 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5694170-5696222(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl C25H3.3
C25H3.14
Entrez Gene 182923 Entrez gene
353396 Entrez gene
SwissProt Q8IG45 EMBL-EBI
Q18187 EMBL-EBI
WormBase CE32139 Wormbase
CE32140 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAN63449 Hypothetical protein C25H3.3 [Caenorhabditis elegans] ref|NP_872068.1| C25H3.3 [Caenorhabditis elegans] 1.0E-79
blast AAN63448 Hypothetical protein C25H3.14 [Caenorhabditis elegans] ref|NP_495115.2| C25H3.14 [Caenorhabditis elegans] 3.0E-76
blast T15630 hypothetical protein C25H3.3 - Caenorhabditis elegans 3.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR006683 EMBL-EBI Thioesterase superfamily 9.7E-16
Pfam IPR006683 EMBL-EBI Thioesterase superfamily 5.1E-11
Sequence
Target Sequence 
>C. ELEGANS:183387_AT
tgatattttcactactggggctcttcttctcacaaaaccagctaggccaggagtatctgt
tgatttgcatgttacatacctgaccgccgctaaaattggtgagacccttgtgctcgactc
caccgtcatcaaacaaggaaaaactcttgcattcacaaaggcagaactctaccgtaaaaa
ttgcttgaaaatggcatcaaaatacatgcaaattgcgaaggaagtgatcaaaatgttcgg
gacaaaaggacaattcggttatgcggctggagcacggaatgtgagagcagttcatgcgga
ggaaggaaatctacgagtggaattcgaagttgaaaaggatcagacaaatcaattcgagac
acttcatggtggatgtacagcagcacttatcgattgtttcacaactggtgctcttctact
cacaaaggaagcgaggcctggtgtttcagtggatttgcatattacatacttgacagctgc
aaacatcggagaaactcttgtactcaactctactgtcatcaaacaggggcgctctcttgg
attcactaaggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATATTTTCACTACTGGGGCTCTT 253 565 213 Antisense
GCTCTTCTTCTCACAAAACCAGCTA 35 301 232 Antisense
AAAACCAGCTAGGCCAGGAGTATCT 530 131 246 Antisense
TTTGCATGTTACATACCTGACCGCC 168 631 276 Antisense
ACCTGACCGCCGCTAAAATTGGTGA 220 1 290 Antisense
GACAATTCGGTTATGCGGCTGGAGC 44 363 461 Antisense
CAGCAGCACTTATCGATTGTTTCAC 310 203 590 Antisense
TTCACAACTGGTGCTCTTCTACTCA 124 687 610 Antisense
GGAAGCGAGGCCTGGTGTTTCAGTG 597 525 639 Antisense
AACTCTTGTACTCAACTCTACTGTC 280 139 705 Antisense
GCGCTCTCTTGGATTCACTAAGGCA 488 287 741 Antisense
Sequence Source Affymetrix Proprietary Database