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[183381_at]

Description C50F7.5 /REP_DB=WormBase Gene ID /WP=CE04243 /TR=Q18751 /GB=AAA83301.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 05      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 04      6grp_total > 05      4grp_total > 54      6grp_highest_sub > De      6grp_high1_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5589
1 2 3 4 range
A 5663.2
(3)
P 		2735
(-0.4)
P 		2169.7
(-1)
P 		450.4
(-3)
P 		5213
B 6030
(3)
P 		2466.6
(-0.9)
P 		1903.9
(-1.5)
P 		480
(-3)
P 		5550
C 5267
(3)
P 		2185.7
(-0.8)
P 		1952.2
(-1.1)
P 		440.8
(-3)
P 		4826
avr 5653.4
(3) 		2462.4
(-0.7) 		2008.6
(-1.2) 		457.1
(-3) 		5196

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5162
1 2 3 4 5 6 range
A 7167.1
(3)
P 		5218.1
(0.2)
P 		4381.2
(-0.9)
P 		3453.1
(-2.3)
P 		4296.4
(-1.1)
P 		2926.6
(-3)
P 		4241
B 6176.1
(3)
P 		4297.2
(-0.3)
P 		4279.5
(-0.4)
P 		3278
(-2.1)
P 		4022.2
(-0.8)
P 		2784.5
(-3)
P 		3392
C 6042.1
(3)
P 		4386.4
(0.5)
P 		5394.2
(2)
P 		2974.2
(-1.6)
P 		4813.7
(1.2)
P 		2005.1
(-3)
P 		4037
avr 6461.8
(3) 		4633.9
(0.2) 		4685
(0.3) 		3235.1
(-2) 		4377.4
(-0.2) 		2572.1
(-3) 		3890
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1996
1 2 3 4 range
A 2062
(3)
P 		593.7
(-1.4)
P 		567.2
(-1.5)
P 		65.9
(-3)
P 		1996
B 1922.9
(3)
P 		609.2
(-1.3)
P 		441.2
(-1.9)
P 		102.3
(-3)
P 		1821
C 1743.5
(3)
P 		628.4
(-1)
P 		680.4
(-0.8)
P 		74.3
(-3)
P 		1669
avr 1909.5
(3) 		610.4
(-1.3) 		562.9
(-1.4) 		80.8
(-3) 		1829

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1641
1 2 3 4 5 6 range
A 1321.2
(0.2)
P 		1855.8
(3)
P 		954.1
(-1.7)
P 		875.8
(-2.1)
P 		1296.5
(0.1)
P 		712.5
(-3)
P 		1143
B 2149
(3)
P 		1430.4
(-0.4)
P 		1039.2
(-2.2)
P 		868.2
(-3)
P 		1634.2
(0.6)
P 		1038.3
(-2.2)
P 		1281
C 2239.5
(3)
P 		1338.4
(-0.3)
P 		1372.9
(-0.2)
P 		598.1
(-3)
P 		1557.6
(0.5)
P 		671.2
(-2.7)
P 		1641
avr 1903.2
(3) 		1541.5
(1.1) 		1122.1
(-1.2) 		780.7
(-3) 		1496.1
(0.8) 		807.3
(-2.9) 		1123
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183381_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8660
Sequence Type Exemplar sequence
Representative Public ID C50F7.5 NCBI
Target Description C50F7.5 /REP_DB=WormBase Gene ID /WP=CE04243 /TR=Q18751 /GB=AAA83301.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068862(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068862 NCBI Caenorhabditis elegans C50F7.5 (C50F7.5) mRNA, complete cds. 11/11 None
SNAP00000024186 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7726703:7727632:1 11/11 None
GENEFINDER00000024199 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7726703:7727632:1 11/11 None
C50F7.5 ENSEMBL cdna:known chromosome:CEL140:IV:7726703:7727682:1 gene:C50F7.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7726708-7727638(+) 97.1 100.0
Public Domain and Genome References
Gene Title
Ensembl C50F7.5
Entrez Gene 177554 Entrez gene
SwissProt Q18751 EMBL-EBI
WormBase CE04243 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA83301 Hypothetical protein C50F7.5 [Caenorhabditis elegans] ref|NP_501263.1| C50F7.5 [Caenorhabditis elegans] 9.0E-40
blast CAE60715 Hypothetical protein CBG04386 [Caenorhabditis briggsae] 6.0E-10
Sequence
Target Sequence 
>C. ELEGANS:183381_AT
tcaccaggacctgtaaatccatccgaagatcctcaaccatctggcccaccatctccggga
ccagtggacccatccgaagatcctcaaccatctgtagagccatccgaagatcatcaacca
tctggcccaccatctccgggaccagtggacccatccgaagatcctcaaccatctgtagag
ccatccgaagatcctcaaccatctggtccaccatctccaggaccagtcgatccatccgaa
gatcctcaaccttctggatcatcatctccaggaccagtggatccatcagatgaaccgtct
ccatctgggccaccatctccgggaccagtagatccatcagaagatcctaaaccttctgaa
ccaccatctccagggccagtagatccatcagatgagccatctccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCACCAGGACCTGTAAATCCATCCG 222 625 328 Antisense
TAAATCCATCCGAAGATCCTCAACC 231 641 341 Antisense
TCTGGTCCACCATCTCCAGGACCAG 330 607 529 Antisense
GACCAGTCGATCCATCCGAAGATCC 218 383 548 Antisense
AGATCCTCAACCTTCTGGATCATCA 438 67 567 Antisense
GATCATCATCTCCAGGACCAGTGGA 190 421 584 Antisense
GGATCCATCAGATGAACCGTCTCCA 694 511 606 Antisense
TCTCCGGGACCAGTAGATCCATCAG 366 609 643 Antisense
GAAGATCCTAAACCTTCTGAACCAC 554 337 667 Antisense
CTCCAGGGCCAGTAGATCCATCAGA 78 237 695 Antisense
GATCCATCAGATGAGCCATCTCCAT 304 415 709 Antisense
Sequence Source Affymetrix Proprietary Database