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[183377_at]

Description Y50E8A.2 /REP_DB=WormBase Gene ID /WP=CE22304 /TR=Q9NAF5 /GB=CAB55045.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 13      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 4
(-3)
A 		15
(3)
A 		12.6
(1.7)
A 		12
(1.4)
A 		11
B 54.2
(3)
A 		23.1
(-3)
A 		26.7
(-2.3)
A 		45.6
(1.3)
A 		31
C 54.4
(-0.3)
A 		16.5
(-2.5)
A 		7.3
(-3)
A 		113.1
(3)
A 		106
avr 37.5
(0.2) 		18.2
(-2.6) 		15.5
(-3) 		56.9
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 91.1
(3)
A 		8.5
(-3)
A 		75.2
(1.8)
A 		11.4
(-2.8)
A 		44.4
(-0.4)
A 		58.7
(0.6)
A 		83
B 9.2
(-2.9)
A 		14.5
(-2.5)
A 		8.4
(-3)
A 		51.3
(0.5)
A 		80.7
(3)
A 		81.2
(3)
A 		73
C 11.8
(-2.4)
A 		7.9
(-2.7)
A 		3.9
(-3)
A 		43.3
(-0)
A 		83.4
(3)
A 		58.7
(1.1)
A 		80
avr 37.4
(-0.3) 		10.3
(-3) 		29.2
(-1.1) 		35.3
(-0.5) 		69.5
(3) 		66.2
(2.7) 		59
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 1.5
(-3)
A 		3.3
(3)
A 		3.3
(3)
A 		1.8
(-2)
A 		2
B 17.3
(3)
A 		5.7
(-3)
A 		6.2
(-2.7)
A 		9.7
(-0.9)
A 		12
C 18
(2.6)
A 		4.7
(-2.2)
A 		2.5
(-3)
A 		19.1
(3)
A 		17
avr 12.3
(3) 		4.6
(-2.6) 		4
(-3) 		10.2
(1.5) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 16.8
(3)
A 		3
(-3)
A 		16.4
(2.8)
A 		2.9
(-3)
A 		13.4
(1.5)
A 		14.3
(1.9)
A 		14
B 3.2
(-2.8)
A 		4.8
(-2.5)
A 		2
(-3)
A 		13.6
(-0.7)
A 		32.8
(3)
A 		30.3
(2.5)
A 		31
C 4.4
(-2.2)
A 		2.4
(-2.7)
A 		1
(-3)
A 		8.7
(-1.2)
A 		27
(3)
A 		19.7
(1.3)
A 		26
avr 8.1
(-1.7) 		3.4
(-3) 		6.5
(-2.1) 		8.4
(-1.6) 		24.4
(3) 		21.4
(2.1) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183377_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13332
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.2 NCBI
Target Description Y50E8A.2 /REP_DB=WormBase Gene ID /WP=CE22304 /TR=Q9NAF5 /GB=CAB55045.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074235(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074235 NCBI Caenorhabditis elegans Y50E8A.2 (Y50E8A.2) mRNA, complete cds. 11/11 None
SNAP00000008977 ENSEMBL cdna:SNAP chromosome:CEL140:V:14731030:14731443:-1 11/11 None
GENEFINDER00000009975 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14731030:14731329:-1 11/11 None
Y50E8A.2 ENSEMBL cdna:known chromosome:CEL140:V:14731030:14731329:-1 gene:Y50E8A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14731029-14731329(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50E8A.2
Entrez Gene 190104 Entrez gene
SwissProt Q9NAF5 EMBL-EBI
WormBase CE22304 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB55045 Hypothetical protein Y50E8A.2 [Caenorhabditis elegans] ref|NP_506636.1| Y50E8A.2 [Caenorhabditis elegans] 1.0E-52
blast CAB55045 Hypothetical protein Y50E8A.2 [Caenorhabditis elegans] ref|NP_506636.1| Y50E8A.2 [Caenorhabditis elegans] 2.0E-52
blast AAF60826 Hypothetical protein Y59E9AR.7 [Caenorhabditis elegans] gb|AAF60828.1| Hypothetical protein Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500755.1| Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500752.1| Y59E9AR.7 [Caenorhabditis elegans] 2.0E-35
blast AAA68714 Major sperm protein protein 63 [Caenorhabditis elegans] ref|NP_495143.1| Major Sperm Protein family member (msp-63) [Caenorhabditis elegans] sp|Q21244|MSP63_CAEEL Major sperm protein 63 (MSP) 2.0E-35
blast AAF60826 Hypothetical protein Y59E9AR.7 [Caenorhabditis elegans] gb|AAF60828.1| Hypothetical protein Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500755.1| Y59E9AR.1 [Caenorhabditis elegans] ref|NP_500752.1| Y59E9AR.7 [Caenorhabditis elegans] 3.0E-27
blast AAA68346 Major sperm protein protein 74 [Caenorhabditis elegans] gb|AAC71085.1| Hypothetical protein ZK1248.4 [Caenorhabditis elegans] gb|AAA96205.1| Hypothetical protein F58A6.9 [Caenorhabditis elegans] ref|NP_495154.1| ZK1248.4 [Caenorhabditis elegans] ref|NP_494960.1| F58A6.9 [Caenorhabditis elegans] ref|NP_494949.1| Major Sperm Protein family member (msp-74) [Caenorhabditis elegans] 3.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 5.8E-9
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.5E-17
Sequence
Target Sequence 
>C. ELEGANS:183377_AT
atgcaccatgtggtgtgctcgacccaaaggaaacagttcttctggatgtttcccgtgatg
ccttcgcattcggacaagaaagcaccaacaacgatcgtttaactgtcgaatggaccaaca
accctgatgaagctgcgaagcaattcagacgcgaagggtgccaaggagacggtatgatcc
gtcgtaagaactcccaactgagtacaacccatctttttaataaatcagtttattttccgc
ttgttttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCACCATGTGGTGTGCTCGACCC 579 43 32 Antisense
GTGCTCGACCCAAAGGAAACAGTTC 657 477 46 Antisense
TCTTCTGGATGTTTCCCGTGATGCC 495 615 69 Antisense
ACAACGATCGTTTAACTGTCGAATG 420 111 119 Antisense
TCGAATGGACCAACAACCCTGATGA 578 599 137 Antisense
ACAACCCTGATGAAGCTGCGAAGCA 461 113 149 Antisense
GACGCGAAGGGTGCCAAGGAGACGG 119 381 179 Antisense
GTATGATCCGTCGTAAGAACTCCCA 36 455 203 Antisense
AGAACTCCCAACTGAGTACAACCCA 509 69 218 Antisense
CTGAGTACAACCCATCTTTTTAATA 143 239 229 Antisense
TCAGTTTATTTTCCGCTTGTTTTCT 55 625 256 Antisense
Sequence Source Affymetrix Proprietary Database