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[183359_at]

Description F58E2.4 /REP_DB=WormBase Gene ID /WP=CE17132 /TR=Q9TYZ8 /GB=AAC68967.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 90      6grp_total > 42      4grp_total > 54      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 149
1 2 3 4 range
A 140.7
(3)
P 		96.7
(0.2)
P 		80
(-0.8)
P 		46
(-3)
A 		95
B 133.3
(3)
P 		113.5
(0.9)
P 		111
(0.6)
P 		77.3
(-3)
A 		56
C 195
(3)
P 		94
(-1.8)
P 		115.2
(-0.8)
P 		67.8
(-3)
P 		127
avr 156.3
(3) 		101.4
(-0.6) 		102.1
(-0.5) 		63.7
(-3) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 219
1 2 3 4 5 6 range
A 176
(1)
P 		161.3
(0.4)
P 		232.5
(3)
P 		135.5
(-0.5)
P 		113.5
(-1.3)
P 		66.1
(-3)
P 		166
B 148.3
(-0.5)
P 		178.4
(0.3)
P 		272.7
(3)
P 		166.4
(0)
P 		113.3
(-1.5)
P 		59.6
(-3)
P 		213
C 128.3
(0.1)
P 		183.3
(2.3)
P 		199.1
(3)
P 		171
(1.8)
P 		79.7
(-1.9)
P 		53.9
(-3)
P 		145
avr 150.9
(0.1) 		174.3
(0.9) 		234.8
(3) 		157.6
(0.4) 		102.2
(-1.5) 		59.9
(-3) 		175
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 51.2
(3)
P 		21
(-1.1)
P 		20.9
(-1.1)
P 		6.7
(-3)
A 		45
B 42.5
(3)
P 		28
(-0.3)
P 		25.7
(-0.9)
P 		16.5
(-3)
A 		26
C 64.5
(3)
P 		27
(-1.2)
P 		40.1
(0.2)
P 		11.4
(-3)
P 		53
avr 52.7
(3) 		25.3
(-1) 		28.9
(-0.5) 		11.5
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 32.4
(-0.6)
P 		57.4
(3)
P 		50.6
(2)
P 		34.4
(-0.3)
P 		34.2
(-0.4)
P 		16.1
(-3)
P 		41
B 51.6
(1)
P 		59.4
(2.1)
P 		66.2
(3)
P 		44.1
(-0)
P 		46
(0.2)
P 		22.2
(-3)
P 		44
C 47.5
(1.7)
P 		55.9
(3)
P 		50.7
(2.2)
P 		34.4
(-0.4)
P 		25.8
(-1.8)
P 		18.1
(-3)
P 		38
avr 43.8
(0.9) 		57.6
(3) 		55.8
(2.7) 		37.6
(-0.1) 		35.3
(-0.4) 		18.8
(-3) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183359_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10801
Sequence Type Exemplar sequence
Representative Public ID F58E2.4 NCBI
Target Description F58E2.4 /REP_DB=WormBase Gene ID /WP=CE17132 /TR=Q9TYZ8 /GB=AAC68967.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067951(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067951 NCBI Caenorhabditis elegans F58E2.4 (F58E2.4) mRNA, complete cds. 11/11 None
SNAP00000029896 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3463932:3466273:1 11/11 None
GENEFINDER00000029900 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3463932:3469776:1 9/11 None
F58E2.4 ENSEMBL cdna:known chromosome:CEL140:IV:3463932:3466273:1 gene:F58E2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3463937-3466279(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58E2.4
Entrez Gene 186520 Entrez gene
SwissProt Q9TYZ8 EMBL-EBI
WormBase CE17132 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68967 Hypothetical protein F58E2.4 [Caenorhabditis elegans] ref|NP_500352.1| F58E2.4 [Caenorhabditis elegans] 0.0
blast AAC68966 Hypothetical protein F58E2.3 [Caenorhabditis elegans] ref|NP_500353.1| F58E2.3 [Caenorhabditis elegans] 0.0
blast CAA21036 Hypothetical protein Y32B12C.3 [Caenorhabditis elegans] ref|NP_507203.1| Y32B12C.3 [Caenorhabditis elegans] 5.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.1E-11
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.0E-26
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.0E-4
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.5E-17
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.5E-20
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 5.5E-5
Sequence
Target Sequence 
>C. ELEGANS:183359_AT
agatacttgtactggcaactagacaattactagacgttatttcgagcgctggaaaaactt
tggatcgccaaatcccagttaaaaagttgagtattcaagcattgggtcataaacccttgt
tgcaaatattaccgttgttaaaccccgtcaccctgaaaactatcgaaattcaaaaatcgg
aagtcattatggccaagaaaccactcaatttaaataaagttgttcaactagaccaatgga
aaaacgcagaagaagttttgataaaatcgattactgctccaggccaaattcaacattttt
tacactttgtggtagcagaggtgaatttagagtcgatttcaatgggagatgttcttgaat
taaagaatgtattccaacagaactcgaacaagctcaaaaagtttaaagctttcttcgaaa
acagtgatattgatgagagaatctatgacgaccaatttcttggatttccttataaacgac
ctgaaaatccatcgacaaatctaccgagtgtttggtttgtaagaataccgacatctactg
ttcttcacattttatattataaaaatcggaaaatgatttccttctcccgtgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATACTTGTACTGGCAACTAGACA 590 63 1451 Antisense
ACTAGACGTTATTTCGAGCGCTGGA 510 101 1479 Antisense
AACTTTGGATCGCCAAATCCCAGTT 296 139 1506 Antisense
GCATTGGGTCATAAACCCTTGTTGC 423 307 1549 Antisense
GATAAAATCGATTACTGCTCCAGGC 467 423 1710 Antisense
ACTGCTCCAGGCCAAATTCAACATT 95 99 1723 Antisense
TCAACATTTTTTACACTTTGTGGTA 356 627 1740 Antisense
TCCAACAGAACTCGAACAAGCTCAA 289 587 1823 Antisense
GAATCTATGACGACCAATTTCTTGG 705 327 1889 Antisense
AATACCGACATCTACTGTTCTTCAC 414 173 1974 Antisense
GGAAAATGATTTCCTTCTCCCGTGT 145 529 2018 Antisense
Sequence Source Affymetrix Proprietary Database