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[183345_at]

Description C54E4.1 /REP_DB=WormBase Gene ID /WP=CE16943 /TR=O44470 /GB=AAK29795.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 36      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 16.9
(-0.2)
A 		18.8
(0.5)
A 		8.7
(-3)
A 		26.2
(3)
A 		18
B 17.7
(1.7)
A 		20.6
(3)
A 		10.4
(-1.4)
A 		6.8
(-3)
A 		14
C 17.2
(-2.8)
A 		18.9
(0.5)
A 		17.1
(-3)
A 		20.2
(3)
A 		3
avr 17.3
(1.3) 		19.4
(3) 		12.1
(-3) 		17.7
(1.6) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 15.5
(1.8)
A 		14.2
(1.1)
A 		9.5
(-1.1)
A 		5.7
(-3)
A 		18
(3)
A 		10.9
(-0.5)
A 		12
B 18.8
(3)
A 		11.4
(-0.6)
A 		6.4
(-3)
A 		9.1
(-1.7)
A 		11.3
(-0.6)
A 		7.1
(-2.7)
A 		12
C 5.3
(-3)
A 		16.9
(3)
A 		14.6
(1.8)
A 		10.1
(-0.5)
A 		10.2
(-0.5)
A 		10.8
(-0.2)
A 		12
avr 13.2
(2) 		14.2
(3) 		10.2
(-1.1) 		8.3
(-3) 		13.2
(2) 		9.6
(-1.7) 		6
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 6.1
(3)
A 		4.1
(-0.2)
A 		2.3
(-3)
A 		3.8
(-0.6)
A 		4
B 5.7
(3)
A 		5.1
(2.2)
A 		2.4
(-1.6)
A 		1.4
(-3)
A 		4
C 5.7
(2.3)
A 		5.4
(1.6)
A 		6
(3)
A 		3.4
(-3)
A 		3
avr 5.8
(3) 		4.9
(1.1) 		3.6
(-1.6) 		2.9
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 2.8
(-0.9)
A 		5.1
(2.6)
A 		2.1
(-2)
A 		1.4
(-3)
A 		5.4
(3)
A 		2.7
(-1.1)
A 		4
B 6.5
(3)
A 		3.8
(-0.3)
A 		1.6
(-3)
A 		2.4
(-2)
A 		4.6
(0.7)
A 		2.7
(-1.7)
A 		5
C 2
(-3)
A 		5.2
(3)
A 		3.7
(0.2)
A 		2
(-3)
A 		3.3
(-0.6)
A 		3.6
(0)
A 		3
avr 3.8
(1.1) 		4.7
(3) 		2.5
(-1.7) 		1.9
(-3) 		4.4
(2.4) 		3
(-0.6) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183345_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10468
Sequence Type Exemplar sequence
Representative Public ID C54E4.1 NCBI
Target Description C54E4.1 /REP_DB=WormBase Gene ID /WP=CE16943 /TR=O44470 /GB=AAK29795.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067772(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067772 NCBI Caenorhabditis elegans C54E4.1 (C54E4.1) mRNA, complete cds. 11/11 None
C54E4.1 ENSEMBL cdna:known chromosome:CEL140:IV:2175605:2177578:1 gene:C54E4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2175610-2177584(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C54E4.1
Entrez Gene 183788 Entrez gene
SwissProt O44470 EMBL-EBI
WormBase CE16943 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29795 Hypothetical protein C54E4.1 [Caenorhabditis elegans] ref|NP_500173.1| C54E4.1 [Caenorhabditis elegans] 4.0E-95
blast CAE68206 Hypothetical protein CBG13874 [Caenorhabditis briggsae] 4.0E-64
Sequence
Target Sequence 
>C. ELEGANS:183345_AT
gtgccacttcgtgtcggaataccaacaggaatgtcaaggaattttcaaagttttggagtg
atgcgggctctataaatagatcaaaatcgtccaatagagcatcaatgaagcagaagcaga
atggaaacggaagcatacgccgaaaccgaacacagtccgtagacgtccgccgagcatcaa
actgtctcccggtccccggtaccccgtcaactatcccgaatccacaattaactcagcgaa
gagccacaagtttccgaagcaatggttcttccacgtcatcaacgcttactgtgaacggag
aggggaggaagtcgaggaaggggagcactacggagctgctcacaccgaattctgcacgat
ctgatatttattcaatagagttcaaccaagcggcgatcgctgccgctcgtctgaaacgcc
tctcgctgacaccgtacccggacccccagctgccgcgtctctccccattacaacaacaac
agccacatcatcgatcgaattccacaaagaagagtgtgacgtcatccgtcagccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCCACTTCGTGTCGGAATACCAA 412 475 134 Antisense
GAAACCGAACACAGTCCGTAGACGT 49 355 275 Antisense
TCAACTATCCCGAATCCACAATTAA 142 631 340 Antisense
GCGAAGAGCCACAAGTTTCCGAAGC 223 285 369 Antisense
TCCACGTCATCAACGCTTACTGTGA 532 589 403 Antisense
GGGAGCACTACGGAGCTGCTCACAC 135 495 454 Antisense
CACACCGAATTCTGCACGATCTGAT 515 193 474 Antisense
TAGAGTTCAACCAAGCGGCGATCGC 492 643 509 Antisense
CTCTCCCCATTACAACAACAACAGC 584 229 592 Antisense
ACAACAGCCACATCATCGATCGAAT 193 113 609 Antisense
AGAGTGTGACGTCATCCGTCAGCCC 299 69 644 Antisense
Sequence Source Affymetrix Proprietary Database