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[183340_at]

Description ZC449.5 /REP_DB=WormBase Gene ID /WP=CE27350 /TR=Q23330 /GB=AAK39385.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 03      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 50      6grp_total > 24      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 464
1 2 3 4 range
A 629.9
(3)
P 		574.7
(1.9)
P 		325.7
(-3)
P 		475.9
(-0)
P 		304
B 658.8
(3)
P 		413.9
(-2.6)
P 		398.4
(-3)
P 		421.1
(-2.5)
P 		260
C 777.5
(3)
P 		489.9
(-0.7)
P 		424.9
(-1.6)
P 		313.5
(-3)
P 		464
avr 688.7
(3) 		492.8
(-0.8) 		383
(-3) 		403.5
(-2.6) 		306

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 610
1 2 3 4 5 6 range
A 724
(3)
P 		646.7
(2.1)
P 		441.3
(-0.3)
P 		311.1
(-1.8)
P 		252
(-2.5)
P 		205.9
(-3)
P 		518
B 647.9
(2.5)
P 		690.3
(3)
P 		474.2
(0.4)
P 		310.3
(-1.5)
P 		264.8
(-2)
P 		182.7
(-3)
P 		508
C 792.4
(3)
P 		678.8
(1.8)
P 		481
(-0.2)
P 		261.5
(-2.5)
P 		251.1
(-2.6)
P 		209.3
(-3)
P 		583
avr 721.4
(3) 		671.9
(2.4) 		465.5
(0.1) 		294.3
(-1.9) 		256
(-2.3) 		199.3
(-3) 		522
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 205
1 2 3 4 range
A 229.3
(3)
P 		124.7
(-0.9)
P 		85.1
(-2.4)
P 		69.7
(-3)
P 		160
B 210.1
(3)
P 		102.2
(-2.4)
P 		92.3
(-2.9)
P 		89.7
(-3)
P 		120
C 257.4
(3)
P 		140.8
(-0.4)
P 		148.1
(-0.2)
P 		52.9
(-3)
P 		205
avr 232.3
(3) 		122.6
(-1.1) 		108.5
(-1.6) 		70.8
(-3) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 244
1 2 3 4 5 6 range
A 133.5
(-0.2)
P 		230
(3)
P 		96.1
(-1.5)
P 		78.9
(-2)
P 		76
(-2.1)
P 		50.1
(-3)
P 		180
B 225.4
(2.8)
P 		229.8
(3)
P 		115.1
(-1.3)
P 		82.2
(-2.5)
P 		107.6
(-1.5)
P 		68.1
(-3)
P 		162
C 293.7
(3)
P 		207.1
(0.8)
P 		122.4
(-1.3)
P 		52.6
(-3)
P 		81.3
(-2.3)
P 		70.1
(-2.6)
P 		241
avr 217.5
(2.8) 		222.3
(3) 		111.2
(-1.2) 		71.2
(-2.7) 		88.3
(-2) 		62.8
(-3) 		160
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183340_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16750
Sequence Type Exemplar sequence
Representative Public ID ZC449.5 NCBI
Target Description ZC449.5 /REP_DB=WormBase Gene ID /WP=CE27350 /TR=Q23330 /GB=AAK39385.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076456(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076456 NCBI Caenorhabditis elegans ZC449.5 (ZC449.5) mRNA, complete cds. 11/11 None
SNAP00000023556 ENSEMBL cdna:SNAP chromosome:CEL140:X:5035484:5037023:-1 11/11 None
GENEFINDER00000023568 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5035484:5037740:-1 11/11 None
ZC449.5.3 ENSEMBL cdna:known chromosome:CEL140:X:5034994:5037742:-1 gene:ZC449.5 11/11 None
ZC449.5.1 ENSEMBL cdna:known chromosome:CEL140:X:5034994:5037740:-1 gene:ZC449.5 11/11 None
ZC449.5.2 ENSEMBL cdna:known chromosome:CEL140:X:5034994:5037742:-1 gene:ZC449.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5035484-5038842(-) 94.19 100.0
Public Domain and Genome References
Gene Title
Ensembl ZC449.5
Entrez Gene 180776 Entrez gene
SwissProt Q23330 EMBL-EBI
WormBase CE27350 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39385 Hypothetical protein ZC449.5 [Caenorhabditis elegans] ref|NP_508857.1| ZC449.5 [Caenorhabditis elegans] 1.0E-52
blast T29919 hypothetical protein ZC449.5 - Caenorhabditis elegans 1.0E-52
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508857.1 1 423-445
ZC449.5.3 1 423-445
ZC449.5.1 1 423-445
ZC449.5.2 1 423-445
SNAP00000023556 1 379-401
GENEFINDER00000023568 1 445-467
Sequence
Target Sequence 
>C. ELEGANS:183340_AT
cctcgtcttcgtccgattcaaagaccgacgaaaaatcgacttcagaatctgattcttcat
cggaagcttcatctgagaagactgtcgagggcactaagaagacattgttctccggaccac
cagactctgatgaggatgatgatgatgaaggagcaggagctgatgaagcattcttctctg
agaaacaagctaccgaggcaccagccgagctctccagaactactactatggctgtgaaga
gcaagaaaatgaagaagtctgagagtggattcaatttcatcccgttggtcattgctggtt
tcgttttctctggattggttggagccggagtttacatgtttaacaaaaagaaggaggaaa
gagaaaacttgtcgacggttgatgagaaagagctgaccacgattgctcctcttcttgagg
atcagaaggagtccgccgaagtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTCGTCTTCGTCCGATTCAAAGAC 394 267 1175 Antisense
TCTTCATCGGAAGCTTCATCTGAGA 263 617 1228 Antisense
TAAGAAGACATTGTTCTCCGGACCA 602 637 1269 Antisense
TCCGGACCACCAGACTCTGATGAGG 306 593 1285 Antisense
GAGAAACAAGCTACCGAGGCACCAG 215 393 1354 Antisense
GGATTCAATTTCATCCCGTTGGTCA 76 509 1441 Antisense
CCGTTGGTCATTGCTGGTTTCGTTT 339 263 1456 Antisense
GGTTGGAGCCGGAGTTTACATGTTT 638 499 1491 Antisense
AAAGAGCTGACCACGATTGCTCCTC 484 123 1561 Antisense
GATTGCTCCTCTTCTTGAGGATCAG 155 433 1575 Antisense
GGATCAGAAGGAGTCCGCCGAAGTC 685 511 1593 Antisense
Sequence Source Affymetrix Proprietary Database