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[183339_at]

Description T21H3.5 /REP_DB=WormBase Gene ID /WP=CE13906 /TR=O16306 /GB=AAB65366.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 99      6grp_total > 99      4grp_total > 19      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 62.5
(-3)
A 		86.3
(0.5)
A 		80.9
(-0.3)
A 		103.3
(3)
P 		41
B 84.7
(-0.1)
P 		95.1
(1.4)
M 		64.5
(-3)
A 		105.8
(3)
P 		41
C 78.2
(-2)
P 		86.4
(-1.6)
A 		59.4
(-3)
A 		176.9
(3)
P 		118
avr 75.1
(-2.3) 		89.3
(-0.9) 		68.3
(-3) 		128.7
(3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 77.8
(0.6)
A 		44.4
(-3)
A 		79.5
(0.7)
P 		60.2
(-1.3)
P 		100.7
(3)
P 		97.7
(2.7)
P 		56
B 65.4
(-0.5)
A 		73.7
(0.7)
P 		47.7
(-3)
A 		74.4
(0.8)
P 		90
(3)
P 		59.8
(-1.3)
P 		42
C 74.2
(0.9)
P 		88.6
(3)
P 		48.2
(-3)
A 		49.1
(-2.9)
A 		81.8
(2)
P 		82.5
(2.1)
P 		40
avr 72.5
(-0.4) 		68.9
(-1.1) 		58.5
(-3) 		61.2
(-2.5) 		90.8
(3) 		80
(1) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 22.7
(3)
A 		18.7
(-0.2)
A 		21.2
(1.8)
A 		15.1
(-3)
P 		8
B 27
(3)
P 		23.5
(1.3)
M 		15
(-3)
A 		22.5
(0.8)
P 		12
C 25.9
(0.4)
P 		24.8
(-0.3)
A 		20.7
(-3)
A 		29.8
(3)
P 		9
avr 25.2
(3) 		22.3
(0.2) 		19
(-3) 		22.5
(0.4) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 14.3
(-3)
A 		15.8
(-2.4)
A 		17.3
(-1.9)
P 		15.3
(-2.6)
P 		30.4
(3)
P 		23.8
(0.5)
P 		16
B 22.8
(-0.3)
A 		24.5
(0.1)
P 		11.6
(-3)
A 		19.7
(-1.1)
P 		36.6
(3)
P 		22.3
(-0.4)
P 		25
C 27.5
(3)
P 		27
(2.8)
P 		12.3
(-2.2)
A 		9.9
(-3)
A 		26.5
(2.6)
P 		27.6
(3)
P 		18
avr 21.5
(-0.3) 		22.4
(-0) 		13.7
(-3) 		15
(-2.6) 		31.2
(3) 		24.6
(0.7) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183339_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15738
Sequence Type Exemplar sequence
Representative Public ID T21H3.5 NCBI
Target Description T21H3.5 /REP_DB=WormBase Gene ID /WP=CE13906 /TR=O16306 /GB=AAB65366.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070986(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070986 NCBI Caenorhabditis elegans T21H3.5 (T21H3.5) mRNA, complete cds. 11/11 None
SNAP00000016910 ENSEMBL cdna:SNAP chromosome:CEL140:V:1158081:1161015:-1 10/11 None
T21H3.5 ENSEMBL cdna:known chromosome:CEL140:V:1157793:1161383:-1 gene:T21H3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1157792-1161383(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T21H3.5
Entrez Gene 188705 Entrez gene
SwissProt O16306 EMBL-EBI
WormBase CE13906 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65366 Hypothetical protein T21H3.5 [Caenorhabditis elegans] ref|NP_503387.1| T21H3.5 [Caenorhabditis elegans] 0.0
blast AAB65366 Hypothetical protein T21H3.5 [Caenorhabditis elegans] ref|NP_503387.1| T21H3.5 [Caenorhabditis elegans] 2.0E-75
blast CAE58024 Hypothetical protein CBG01096 [Caenorhabditis briggsae] 2.0E-47
blast CAE58024 Hypothetical protein CBG01096 [Caenorhabditis briggsae] 4.0E-21
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503387.1 7 13-35,48-70,100-122,144-163,197-219,249-271,281-303
T21H3.5 7 13-35,48-70,100-122,144-163,197-219,249-271,281-303
SNAP00000016910 3 10-29,59-81,91-113
Sequence
Target Sequence 
>C. ELEGANS:183339_AT
agcggtcacgacttttcatattttgtggttgattggagtttctcgttgctagttttcaca
tttaccatcccctacattacttgctttatcgttagtctggtttttcataaattttatatg
aaatcgattcggaacgtttccaacgagttcatcgatatgggatatcacgactcggcgaac
atcaatagaaccctcctatcaattgctcttcagaatctcatatcaatttccattccacac
tttgtgctcacaattacgtctcttctgaaatgggatcttggtgtccgtgcgttaccggct
tcgtttggatgtattacgattacattgacaacgtctctcacaatttttgtcaattttaga
gcttacagacgacaactcggcttcattataatcgattttttccggcaaaaaaatgtgaac
ctttccgtctttgtcgtcgataatcagcagcaagccgatcaacttcaaatgtccaccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCGGTCACGACTTTTCATATTTTG 642 79 565 Antisense
GTTTCTCGTTGCTAGTTTTCACATT 151 451 602 Antisense
TCCCCTACATTACTTGCTTTATCGT 21 597 632 Antisense
GGGATATCACGACTCGGCGAACATC 588 493 723 Antisense
ATAGAACCCTCCTATCAATTGCTCT 122 21 749 Antisense
TTCCATTCCACACTTTGTGCTCACA 303 701 792 Antisense
TGCTCACAATTACGTCTCTTCTGAA 194 581 809 Antisense
AAATGGGATCTTGGTGTCCGTGCGT 261 121 832 Antisense
ACGACAACTCGGCTTCATTATAATC 66 87 933 Antisense
CCTTTCCGTCTTTGTCGTCGATAAT 55 269 984 Antisense
GCCGATCAACTTCAAATGTCCACCG 206 285 1018 Antisense
Sequence Source Affymetrix Proprietary Database