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[183333_at]

Description C33H5.3 /REP_DB=WormBase Gene ID /WP=CE04147 /TR=Q18414 /GB=AAA82263.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 12      6grp_cgc > 58      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 59.6
(1.2)
A 		27.4
(-1.2)
A 		4
(-3)
A 		83
(3)
P 		79
B 33.7
(-1.4)
A 		56.4
(0.5)
P 		15.4
(-3)
A 		86.1
(3)
A 		71
C 46.1
(-2.3)
A 		36.2
(-3)
A 		50.6
(-2)
A 		119.2
(3)
M 		83
avr 46.5
(-1.1) 		40
(-1.6) 		23.3
(-3) 		96.1
(3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 44.5
(-1.7)
A 		30
(-2.5)
A 		21.5
(-3)
A 		108.3
(1.8)
P 		45.7
(-1.7)
P 		129.8
(3)
P 		108
B 29.8
(-2.8)
P 		23
(-3)
A 		50.6
(-2.1)
A 		47.1
(-2.2)
P 		90.9
(-0.7)
P 		202.8
(3)
P 		180
C 15.8
(-2.9)
A 		50.4
(-2)
P 		50.3
(-2)
A 		13.7
(-3)
A 		115.9
(-0.2)
P 		233.5
(3)
P 		220
avr 30
(-3) 		34.5
(-2.8) 		40.8
(-2.6) 		56.4
(-2) 		84.2
(-1) 		188.7
(3) 		159
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 21.7
(3)
A 		5.9
(-1.6)
A 		1.1
(-3)
A 		12.1
(0.2)
P 		21
B 10.7
(-0.1)
A 		13.9
(1.2)
P 		3.6
(-3)
A 		18.4
(3)
A 		15
C 15.3
(0)
A 		10.4
(-3)
A 		17.6
(1.5)
A 		20.1
(3)
M 		10
avr 15.9
(2.4) 		10.1
(-1.3) 		7.4
(-3) 		16.9
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 8.2
(-2.2)
A 		10.7
(-1.7)
A 		4.7
(-3)
A 		27.5
(2.1)
P 		13.8
(-1)
P 		31.6
(3)
P 		27
B 10.4
(-2.8)
P 		7.6
(-3)
A 		12.3
(-2.6)
A 		12.5
(-2.6)
P 		36.9
(-0.4)
P 		75.6
(3)
P 		68
C 5.8
(-2.8)
A 		15.4
(-2)
P 		12.8
(-2.2)
A 		2.8
(-3)
A 		37.5
(-0.2)
P 		78.2
(3)
P 		75
avr 8.1
(-3) 		11.2
(-2.7) 		9.9
(-2.8) 		14.3
(-2.3) 		29.4
(-0.6) 		61.8
(3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183333_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10138
Sequence Type Exemplar sequence
Representative Public ID C33H5.3 NCBI
Target Description C33H5.3 /REP_DB=WormBase Gene ID /WP=CE04147 /TR=Q18414 /GB=AAA82263.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068890(11), NM_001027873(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068890 NCBI Caenorhabditis elegans C33H5.3 (C33H5.3) mRNA, complete cds. 11/11 A 
NM_001027873 NCBI Caenorhabditis elegans Kinesin-Like Protein family member (klp-10) (klp-10) mRNA, complete cds. 11/11 None
SNAP00000004486 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7799816:7800235:1 11/11 None
GENEFINDER00000004495 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7797175:7800235:1 11/11 None
C33H5.4a ENSEMBL cdna:known chromosome:CEL140:IV:7797104:7800382:1 gene:C33H5.4 11/11 A 
C33H5.3 ENSEMBL cdna:known chromosome:CEL140:IV:7799816:7800235:1 gene:C33H5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
3565411 2 1 NM_001027873 183333_at (11), 188130_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7799821-7800241(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C33H5.4
C33H5.3
Entrez Gene 183185 Entrez gene
SwissProt Q18414 EMBL-EBI
WormBase CE04147 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82277 Kinesin-like protein protein 10, isoform a [Caenorhabditis elegans] ref|NP_001023044.1| Kinesin-Like Protein family member (klp-10) [Caenorhabditis elegans] dbj|BAB82459.1| Kinesin like protein [Caenorhabditis elegans] 0.0
blast AAB03132 Kinesin-like protein protein 18 [Caenorhabditis elegans] ref|NP_501093.1| Kinesin-Like Protein family member (klp-18) [Caenorhabditis elegans] gb|AAO34669.1| kinesin-like protein-18 [Caenorhabditis elegans] 0.0
blast BAB19356.2 kinesin like protein KLP-18 [Caenorhabditis elegans] 0.0
blast AAA82263 Hypothetical protein C33H5.3 [Caenorhabditis elegans] ref|NP_501291.2| C33H5.3 [Caenorhabditis elegans] 1.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.5E-114
Sequence
Target Sequence 
>C. ELEGANS:183333_AT
caaagagaaactttccgttagcttgaaaaaacaagaagacaccattcgaaggcagagcct
ggctgagaatcaaggaaagctagaagtaatggaaaatatgaatcgcgtgctttctgaggc
aattgaatctcttcaatcgcagcattccaaggatacagagatcattaaggcagggctgcg
tacaaaaggagatcgggtgacttacgtggataatcttcgcgtcgagaagaaagctctcga
agaaaccgtttctcaacttcgctctgagaatgataaactgaagaagatcatcagaggaga
atcgaccaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAGAGAAACTTTCCGTTAGCTTG 686 189 42 Antisense
AGACACCATTCGAAGGCAGAGCCTG 351 71 78 Antisense
GCAGAGCCTGGCTGAGAATCAAGGA 53 313 93 Antisense
GAATCGCGTGCTTTCTGAGGCAATT 63 329 141 Antisense
TTCTGAGGCAATTGAATCTCTTCAA 10 695 153 Antisense
TCAATCGCAGCATTCCAAGGATACA 276 633 174 Antisense
AGAGATCATTAAGGCAGGGCTGCGT 37 67 198 Antisense
GGCAGGGCTGCGTACAAAAGGAGAT 664 535 210 Antisense
AAAAGGAGATCGGGTGACTTACGTG 174 127 225 Antisense
GGTGACTTACGTGGATAATCTTCGC 400 499 237 Antisense
ATCATCAGAGGAGAATCGACCAATA 169 29 328 Antisense
Sequence Source Affymetrix Proprietary Database