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[183320_at]

Description F45E4.5 /REP_DB=WormBase Gene ID /WP=CE24982 /TR=Q94228 /GB=AAK29816.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 29      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 76      6grp_total > 28      4grp_total > 33      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 875
1 2 3 4 range
A 359.3
(-3)
P 		1075.5
(3)
P 		760.8
(0.4)
P 		818.7
(0.8)
P 		716
B 373.4
(-3)
P 		724.5
(1.1)
P 		891.2
(3)
P 		777.3
(1.7)
P 		518
C 578.2
(-3)
P 		887.2
(-0.2)
P 		674.7
(-2.1)
P 		1233.8
(3)
P 		656
avr 437
(-3) 		895.7
(2.4) 		775.6
(1) 		943.3
(3) 		506

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 701
1 2 3 4 5 6 range
A 1328.9
(3)
P 		1081.1
(0.1)
P 		857.8
(-2.5)
P 		1037.6
(-0.4)
P 		814.4
(-3)
P 		1058.5
(-0.2)
P 		515
B 1109.1
(1.8)
P 		1145.8
(2.6)
P 		1051.6
(0.6)
P 		885.4
(-3)
P 		1083.4
(1.3)
P 		1163.3
(3)
P 		278
C 1381.5
(3)
P 		1060.1
(0.2)
P 		745.2
(-2.5)
P 		681
(-3)
P 		1121.6
(0.8)
P 		865.9
(-1.4)
P 		701
avr 1273.2
(3) 		1095.7
(0.4) 		884.9
(-2.7) 		868
(-3) 		1006.5
(-0.9) 		1029.2
(-0.6) 		405
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 130.8
(-2.4)
P 		233.5
(3)
P 		198.9
(1.2)
P 		119.8
(-3)
P 		114
B 119.1
(-3)
P 		178.9
(1.1)
P 		206.5
(3)
P 		165.7
(0.2)
P 		87
C 191.4
(-3)
P 		255.1
(3)
P 		235.2
(1.1)
P 		208
(-1.4)
P 		64
avr 147.1
(-3) 		222.5
(3) 		213.5
(2.3) 		164.5
(-1.6) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 375
1 2 3 4 5 6 range
A 245
(-1.2)
P 		384.5
(3)
P 		186.8
(-3)
P 		263.2
(-0.7)
P 		245.7
(-1.2)
P 		257.7
(-0.8)
P 		198
B 385.9
(1.4)
P 		381.4
(1.3)
P 		255.4
(-2.4)
P 		234.5
(-3)
P 		440.2
(3)
P 		433.8
(2.8)
P 		206
C 512.1
(3)
P 		323.5
(-0)
P 		189.7
(-2.2)
P 		137
(-3)
P 		362.9
(0.6)
P 		289.9
(-0.6)
P 		375
avr 381
(3) 		363.1
(2.4) 		210.6
(-3) 		211.6
(-3) 		349.6
(1.9) 		327.1
(1.1) 		170
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183320_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8600
Sequence Type Exemplar sequence
Representative Public ID F45E4.5 NCBI
Target Description F45E4.5 /REP_DB=WormBase Gene ID /WP=CE24982 /TR=Q94228 /GB=AAK29816.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068838(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068838 NCBI Caenorhabditis elegans F45E4.5 (F45E4.5) mRNA, complete cds. 10/11 None
GENEFINDER00000024183 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7628319:7628972:-1 9/11 None
F45E4.5 ENSEMBL cdna:known chromosome:CEL140:IV:7628294:7628883:-1 gene:F45E4.5 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000024162 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7628278-7628889(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45E4.5
Entrez Gene 185796 Entrez gene
SwissProt Q94228 EMBL-EBI
WormBase CE35313 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29816 Hypothetical protein F45E4.5 [Caenorhabditis elegans] ref|NP_501239.2| F45E4.5 [Caenorhabditis elegans] 1.0E-37
blast CAE61927 Hypothetical protein CBG05924 [Caenorhabditis briggsae] 6.0E-28
blast CAE61927 Hypothetical protein CBG05924 [Caenorhabditis briggsae] 7.0E-28
Sequence
Target Sequence 
>C. ELEGANS:183320_AT
aagaaggaggtatggctgagctgaaatcaataacaggagatgctccatacgatggtgttg
gaaatgcaccagcacctattccacagccaatgggattctggggaagaccaagatattctc
caccaggccatggatttgatggtccatatggaggcccaatgatgggtggaggtggcccat
tcccatattctccatacaacagatttacaagcatgtcaagcagtccatatggggtacatg
gtacaacaacctccaataatttttcatttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAAGGAGGTATGGCTGAGCTGAA 624 149 32 Antisense
GGAGATGCTCCATACGATGGTGTTG 49 519 67 Antisense
GTTGGAAATGCACCAGCACCTATTC 197 437 88 Antisense
GCACCTATTCCACAGCCAATGGGAT 428 313 103 Antisense
GGGGAAGACCAAGATATTCTCCACC 144 497 131 Antisense
ATTCTCCACCAGGCCATGGATTTGA 317 9 146 Antisense
GATGGTCCATATGGAGGCCCAATGA 501 407 169 Antisense
ATTCCCATATTCTCCATACAACAGA 441 9 210 Antisense
GCATGTCAAGCAGTCCATATGGGGT 140 311 242 Antisense
GGGTACATGGTACAACAACCTCCAA 482 497 263 Antisense
CAACCTCCAATAATTTTTCATTTCT 395 187 278 Antisense
Sequence Source Affymetrix Proprietary Database