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[183316_at]

Description F29C4.5 /REP_DB=WormBase Gene ID /WP=CE17722 /TR=O76364 /GB=AAC19188.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 83      4grp_total > 43      6grp_cgc > 87      6grp_cgc_sub > 05      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 19.1
(-3)
A 		104.1
(3)
A 		57.5
(-0.3)
A 		21.3
(-2.8)
A 		85
B 73.2
(3)
A 		27.5
(-3)
A 		40.1
(-1.3)
A 		51.2
(0.1)
A 		46
C 42.9
(1.3)
A 		43.6
(1.4)
A 		14.8
(-3)
A 		54.4
(3)
A 		40
avr 45.1
(-0.8) 		58.4
(3) 		37.5
(-3) 		42.3
(-1.6) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 21.5
(-3)
A 		30.7
(-1.4)
A 		42.1
(0.6)
A 		56.2
(3)
A 		49.3
(1.8)
A 		44.6
(1)
A 		35
B 20.1
(-2.6)
A 		14.7
(-3)
A 		92.5
(3)
A 		60.1
(0.5)
A 		43.5
(-0.8)
A 		28.1
(-2)
A 		78
C 74.8
(0.4)
A 		17.4
(-2.9)
A 		15
(-3)
A 		52
(-0.9)
A 		121.4
(3)
P 		57
(-0.6)
A 		106
avr 38.8
(-0.9) 		20.9
(-3) 		49.9
(0.4) 		56.1
(1.2) 		71.4
(3) 		43.2
(-0.4) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 6.9
(-1.8)
A 		22.6
(3)
A 		15
(0.7)
A 		3.1
(-3)
A 		20
B 23.4
(3)
A 		6.8
(-3)
A 		9.3
(-2.1)
A 		10.9
(-1.5)
A 		17
C 14.2
(3)
A 		12.5
(1.9)
A 		5.1
(-3)
A 		9.2
(-0.3)
A 		9
avr 14.8
(3) 		14
(2.3) 		9.8
(-1.2) 		7.7
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 4
(-3)
A 		10.9
(0.8)
A 		9.2
(-0.1)
A 		14.3
(2.7)
A 		14.9
(3)
A 		10.8
(0.7)
A 		11
B 7
(-2.3)
A 		4.9
(-3)
A 		22.5
(3)
A 		15.9
(0.8)
A 		17.7
(1.4)
A 		10.5
(-1.1)
A 		18
C 27.7
(1)
A 		5.3
(-2.7)
A 		3.8
(-3)
A 		10.5
(-1.9)
A 		39.3
(3)
P 		19.1
(-0.4)
A 		36
avr 12.9
(-0.9) 		7
(-3) 		11.8
(-1.3) 		13.6
(-0.7) 		24
(3) 		13.5
(-0.7) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183316_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10581
Sequence Type Exemplar sequence
Representative Public ID F29C4.5 NCBI
Target Description F29C4.5 /REP_DB=WormBase Gene ID /WP=CE17722 /TR=O76364 /GB=AAC19188.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067463(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067463 NCBI Caenorhabditis elegans Deubiquitylating with USP/UBP and OTU domains family member (duo-2) (duo-2) mRNA, complete cds. 11/11 None
SNAP00000028007 ENSEMBL cdna:SNAP chromosome:CEL140:IV:112410:114424:-1 11/11 None
GENEFINDER00000028020 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:112410:114957:-1 11/11 None
F29C4.5 ENSEMBL cdna:known chromosome:CEL140:IV:112410:114957:-1 gene:F29C4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:112409-114957(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol duo-2
Ensembl F29C4.5
Entrez Gene 185111 Entrez gene
SwissProt O76364 EMBL-EBI
WormBase CE17722 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4221 ubiquitin thiolesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC19188 Deubiquitylating with usp/ubp and otu domains protein 2 [Caenorhabditis elegans] ref|NP_499864.1| Deubiquitylating with USP/UBP and OTU domains family member (duo-2) [Caenorhabditis elegans] 0.0
blast CAA98471 Hypothetical protein F38B7.5 [Caenorhabditis elegans] ref|NP_505931.2| Deubiquitylating with USP/UBP and OTU domains family member (duo-1) [Caenorhabditis elegans] dbj|BAC22611.1| ubiquitin-specific protease [Caenorhabditis elegans] 0.0
blast T21950 hypothetical protein F38B7.5 - Caenorhabditis elegans 1.0E-180
blast T21950 hypothetical protein F38B7.5 - Caenorhabditis elegans 1.0E-174
blast T21950 hypothetical protein F38B7.5 - Caenorhabditis elegans 1.0E-166
blast CAA98471 Hypothetical protein F38B7.5 [Caenorhabditis elegans] ref|NP_505931.2| Deubiquitylating with USP/UBP and OTU domains family member (duo-1) [Caenorhabditis elegans] dbj|BAC22611.1| ubiquitin-specific protease [Caenorhabditis elegans] 1.0E-130
Protein Domains
Database ID Description E-Value
Pfam IPR003323 EMBL-EBI Ovarian tumour, otubain 1.0E-22
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 4.9E-36
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.8E-61
Pfam IPR003323 EMBL-EBI Ovarian tumour, otubain 2.3E-34
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 8.3E-19
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 2.5E-60
Pfam IPR003323 EMBL-EBI Ovarian tumour, otubain 2.3E-34
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 2.5E-60
Sequence
Target Sequence 
>C. ELEGANS:183316_AT
gccgacgggaattgcttctatcgtgcagtttcgtggtggcttaccggagtggaatcgcat
catatgatcttccgcgaagcagtcggcaaacatctcaagaagaatgaaccaaagttcaaa
aagtactgtcatgatgagatatacgaaaagtacgttgacaacgtaatgcgtgaaggagtt
tgggcatcaacttatagtggatggatgcctcattctccacaaaatacgtgtccaccaaga
caaggagcattatatttgaaaaatacacatatgcattatgagtccacaatctcattgaaa
aagaatacttcaatgaagtcgacaatgacatcgcaaaaaggaaacaataatgagcgttta
atgcacggagagctgcacgaaaaggataatatgagaggcgatgcgcctcctagaagatat
acactggtttcggttgtatgtcacctcggaagttcgccgaacaaaggacatcacgttgcc
tacacaaagaaatcgctcgacaacgatggctggctgcgctgctccgatgataaattacgt
gatgtatcgaaagaaaacgtggccaacgctgtccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCGACGGGAATTGCTTCTATCGTG 170 285 1000 Antisense
TATCGTGCAGTTTCGTGGTGGCTTA 148 657 1018 Antisense
GGTGGCTTACCGGAGTGGAATCGCA 501 499 1034 Antisense
GGATGCCTCATTCTCCACAAAATAC 511 513 1202 Antisense
GATGCGCCTCCTAGAAGATATACAC 126 415 1399 Antisense
TGGTTTCGGTTGTATGTCACCTCGG 179 551 1424 Antisense
GTCACCTCGGAAGTTCGCCGAACAA 576 463 1439 Antisense
AAAGGACATCACGTTGCCTACACAA 129 121 1462 Antisense
CGCTCGACAACGATGGCTGGCTGCG 525 255 1493 Antisense
GCTGCGCTGCTCCGATGATAAATTA 249 297 1512 Antisense
AAGAAAACGTGGCCAACGCTGTCCG 229 151 1550 Antisense
Sequence Source Affymetrix Proprietary Database