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[183294_at]

Description F58F9.2 /REP_DB=WormBase Gene ID /WP=CE07299 /TR=Q20990 /GB=AAC48083.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 80      6grp_total > 62      4grp_total > 08      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 168
1 2 3 4 range
A 58.5
(-3)
P 		113.1
(-0)
P 		118.7
(0.3)
P 		168.5
(3)
P 		110
B 136.6
(-2.3)
P 		125.8
(-3)
P 		193.4
(1.3)
P 		220.5
(3)
P 		95
C 123.6
(-2.7)
P 		118.7
(-3)
P 		204.4
(1.8)
P 		226.7
(3)
P 		108
avr 106.2
(-3) 		119.2
(-2.2) 		172.2
(1) 		205.2
(3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 290
1 2 3 4 5 6 range
A 67.1
(-3)
P 		154.5
(-1.1)
P 		350.2
(3)
P 		241.9
(0.7)
P 		216.9
(0.2)
P 		106.8
(-2.2)
P 		283
B 197.4
(-2.8)
P 		190.9
(-3)
P 		357.4
(3)
P 		327.7
(1.9)
P 		234
(-1.4)
P 		220.6
(-1.9)
P 		167
C 151
(-3)
P 		267.3
(3)
P 		204.6
(-0.2)
P 		231.1
(1.1)
P 		191.2
(-0.9)
P 		212.7
(0.2)
P 		116
avr 138.5
(-3) 		204.2
(-0.6) 		304.1
(3) 		266.9
(1.7) 		214
(-0.3) 		180
(-1.5) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 21.3
(-3)
P 		24.5
(-1)
P 		31
(3)
P 		24.7
(-0.9)
P 		10
B 43.6
(1.7)
P 		31.1
(-3)
P 		44.8
(2.2)
P 		47
(3)
P 		16
C 40.9
(-1.9)
P 		34.1
(-3)
P 		71.2
(3)
P 		38.2
(-2.3)
P 		37
avr 35.3
(-1.3) 		29.9
(-3) 		49
(3) 		36.6
(-0.9) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 12.4
(-3)
P 		55
(1)
P 		76.3
(3)
P 		61.4
(1.6)
P 		65.5
(2)
P 		26
(-1.7)
P 		64
B 68.7
(-2)
P 		63.6
(-3)
P 		86.8
(1.4)
P 		86.8
(1.4)
P 		95.1
(3)
P 		82.2
(0.5)
P 		32
C 56
(-1.4)
P 		81.6
(3)
P 		52.1
(-2)
P 		46.5
(-3)
P 		61.9
(-0.4)
P 		71.2
(1.2)
P 		35
avr 45.7
(-3) 		66.7
(1.4) 		71.7
(2.5) 		64.9
(1) 		74.2
(3) 		59.8
(-0) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183294_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10527
Sequence Type Exemplar sequence
Representative Public ID F58F9.2 NCBI
Target Description F58F9.2 /REP_DB=WormBase Gene ID /WP=CE07299 /TR=Q20990 /GB=AAC48083.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028071(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028071 NCBI Caenorhabditis elegans F58F9.3b (F58F9.3) mRNA, complete cds. 11/11 None
SNAP00000041825 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6234943:6235545:1 11/11 None
GENEFINDER00000041839 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6231028:6235545:1 11/11 None
F58F9.3b ENSEMBL cdna:known chromosome:CEL140:IV:6233237:6235591:1 gene:F58F9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
186534 2 1 NM_001028071 183294_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6234948-6235551(+) 100.0 100.0
Public Domain and Genome References
Ensembl F58F9.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48083 Hypothetical protein F58F9.3b [Caenorhabditis elegans] ref|NP_001023242.1| F58F9.3b [Caenorhabditis elegans] 0.0
blast AAC48085 Hypothetical protein F58F9.4 [Caenorhabditis elegans] ref|NP_500939.1| F58F9.4 [Caenorhabditis elegans] 1.0E-167
blast AAC48083 Hypothetical protein F58F9.3b [Caenorhabditis elegans] ref|NP_001023242.1| F58F9.3b [Caenorhabditis elegans] 1.0E-162
blast AAC48084 Hypothetical protein F58F9.3a [Caenorhabditis elegans] ref|NP_001023241.1| F58F9.3a [Caenorhabditis elegans] 2.0E-95
blast AAC48083 Hypothetical protein F58F9.3b [Caenorhabditis elegans] ref|NP_001023242.1| F58F9.3b [Caenorhabditis elegans] 3.0E-81
blast AAC48084 Hypothetical protein F58F9.3a [Caenorhabditis elegans] ref|NP_001023241.1| F58F9.3a [Caenorhabditis elegans] 3.0E-79
blast AAC48085 Hypothetical protein F58F9.4 [Caenorhabditis elegans] ref|NP_500939.1| F58F9.4 [Caenorhabditis elegans] 5.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR004987 EMBL-EBI Protein of unknown function DUF272 6.8E-84
Pfam IPR004987 EMBL-EBI Protein of unknown function DUF272 6.8E-84
Pfam IPR004987 EMBL-EBI Protein of unknown function DUF272 1.5E-69
Pfam IPR004987 EMBL-EBI Protein of unknown function DUF272 6.8E-84
Sequence
Target Sequence 
>C. ELEGANS:183294_AT
agcttttgtgtacagcacaatccaaaaaaaagatctcaatacgtattttttgtggatttg
tgacaaacaagaaaaatcaatttttacttcgaaaactcatgtgcttggactagggcactt
ctttgaaggatattttgaacaaacgccaaatggaaagtctcaatgggaatgtgtcaaata
tttgaaacagatagatcaactcatacaaggagatgtaattagaaataaagttgtgttgag
aacgagagtaactcagtatgagccggctactggtcatagaaagtatccacaagtttatgc
caatcatctcgggatcattattgacaatcaaaataaacttccgatctactgcaacggtgt
gcaaatcaaaactca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTTTTGTGTACAGCACAATCCAA 363 79 18 Antisense
ATACGTATTTTTTGTGGATTTGTGA 295 23 56 Antisense
TACTTCGAAAACTCATGTGCTTGGA 492 641 102 Antisense
GCTTGGACTAGGGCACTTCTTTGAA 697 305 120 Antisense
GGAAAGTCTCAATGGGAATGTGTCA 302 527 169 Antisense
GAGTAACTCAGTATGAGCCGGCTAC 73 399 263 Antisense
GAGCCGGCTACTGGTCATAGAAAGT 111 385 277 Antisense
AAGTATCCACAAGTTTATGCCAATC 236 157 298 Antisense
TATGCCAATCATCTCGGGATCATTA 223 657 313 Antisense
AACTTCCGATCTACTGCAACGGTGT 98 139 353 Antisense
GCAACGGTGTGCAAATCAAAACTCA 202 319 368 Antisense
Sequence Source Affymetrix Proprietary Database