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[183279_at] 7TM chemoreceptor, srb family

Description C27D6.9 /REP_DB=WormBase Gene ID /WP=CE06889 /GEN=srb-2 /GB=AAK68210.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 34      4grp_total > 12      6grp_cgc > 44      6grp_cgc_sub > 02      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 5.8
(-2.5)
A 		9.2
(-2.1)
A 		1.7
(-3)
A 		53.9
(3)
A 		52
B 58.3
(3)
A 		31.2
(-0.3)
A 		9.5
(-3)
A 		43.5
(1.2)
A 		49
C 5.3
(-2.7)
A 		6
(-2.7)
A 		2.5
(-3)
A 		68.5
(3)
P 		66
avr 23.1
(-0.8) 		15.5
(-1.7) 		4.6
(-3) 		55.3
(3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 33.1
(2.1)
A 		2.8
(-3)
A 		38.5
(3)
A 		11
(-1.6)
A 		2.8
(-3)
A 		4.3
(-2.7)
A 		36
B 13.4
(-0)
A 		23.4
(3)
A 		13.5
(0)
A 		3.7
(-3)
A 		3.6
(-3)
A 		3.6
(-3)
A 		20
C 6.2
(-2.3)
A 		46.2
(3)
A 		33.2
(1.3)
A 		2.4
(-2.8)
A 		1.1
(-3)
A 		3.4
(-2.7)
A 		45
avr 17.6
(0.5) 		24.1
(2) 		28.4
(3) 		5.7
(-2.3) 		2.5
(-3) 		3.8
(-2.7) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 2.1
(-1.6)
A 		2
(-1.7)
A 		0.4
(-3)
A 		7.9
(3)
A 		8
B 18.6
(3)
A 		7.7
(-1)
A 		2.2
(-3)
A 		9.3
(-0.4)
A 		16
C 1.8
(-2.5)
A 		1.7
(-2.6)
A 		0.9
(-3)
A 		11.6
(3)
P 		11
avr 7.5
(1.5) 		3.8
(-1.1) 		1.2
(-3) 		9.6
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 6.1
(1.2)
A 		1
(-2.8)
A 		8.4
(3)
A 		2.8
(-1.4)
A 		0.8
(-3)
A 		1
(-2.8)
A 		8
B 4.7
(0.3)
A 		7.8
(3)
A 		3.3
(-1)
A 		1
(-3)
A 		1.5
(-2.6)
A 		1.3
(-2.7)
A 		7
C 2.3
(-2.1)
A 		14.1
(3)
A 		8.5
(0.6)
A 		0.5
(-2.9)
A 		0.3
(-3)
A 		1.1
(-2.7)
A 		14
avr 4.4
(0.1) 		7.6
(3) 		6.7
(2.2) 		1.4
(-2.6) 		0.9
(-3) 		1.1
(-2.8) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183279_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5185
Sequence Type Exemplar sequence
Representative Public ID C27D6.9 NCBI
Target Description C27D6.9 /REP_DB=WormBase Gene ID /WP=CE06889 /GEN=srb-2 /GB=AAK68210.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062563(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062563 NCBI Caenorhabditis elegans Serpentine Receptor, class B (beta) family member (srb-2) (srb-2) mRNA, complete cds. 11/11 None
C27D6.9 ENSEMBL cdna:known chromosome:CEL140:II:5168130:5169305:-1 gene:C27D6.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000035753 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5168127-5169303(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srb family
Gene Symbol srb-2
Ensembl C27D6.9
Entrez Gene 191784 Entrez gene
WormBase CE38151 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494964 Serpentine Receptor, class B (beta) family member (srb-2) [Caenorhabditis elegans] gb|AAK68210.2| Serpentine receptor, class b (beta) protein 2 [Caenorhabditis elegans] 0.0
blast Q95ZY1 Serpentine receptor class beta-2 (Protein srb-2) prf||2123261P chemosensory receptor 0.0
blast NP_494965 Serpentine Receptor, class B (beta) family member (srb-1) [Caenorhabditis elegans] gb|AAK68205.1| Serpentine receptor, class b (beta) protein 1 [Caenorhabditis elegans] sp|Q95ZY0|SRB1_CAEEL Serpentine receptor class beta-1 (Protein srb-1) prf||2123261N chemosensory receptor 1.0E-145
blast NP_494965 Serpentine Receptor, class B (beta) family member (srb-1) [Caenorhabditis elegans] gb|AAK68205.1| Serpentine receptor, class b (beta) protein 1 [Caenorhabditis elegans] sp|Q95ZY0|SRB1_CAEEL Serpentine receptor class beta-1 (Protein srb-1) prf||2123261N chemosensory receptor 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR002184 EMBL-EBI Integral membrane protein Srb, nematode type 1.0E-126
Pfam IPR002184 EMBL-EBI Integral membrane protein Srb, nematode type 2.7E-119
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494964.2 7 25-47,60-82,112-134,146-168,193-215,248-270,285-307
C27D6.9 7 25-47,60-82,102-124,136-158,183-205,238-260,275-297
Sequence
Target Sequence 
>C. ELEGANS:183279_AT
tgttcggacatttttgggaccgctcctagttttcacattgattggaattgacttggcttt
actttaccatgtatttcgagatgaaaagtttgaagatagtttcatatcatttgcccttgt
ccctgagacctcagccataccattcaatagctatttttgggagcttttatatgcagaaat
tggaaatttcatttgcaattgcatatttctattggttcacagcaaatttaaagcaagatt
tctccatcaacaacgatccttatcagtaagatatttattggaagaaatatcccaaacttc
aaaattcacattgattgttagcttcacacatcttctatttgttggatggtaccttattgc
aacaatttttgtgagaactattggtgaagactttttcggaggatatattcattatacagt
ggcaagaggcgttcatattactgtgccaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTCGGACATTTTTGGGACCGCTC 644 557 408 Antisense
CCGCTCCTAGTTTTCACATTGATTG 255 263 427 Antisense
GATTGGAATTGACTTGGCTTTACTT 567 435 447 Antisense
GGCTTTACTTTACCATGTATTTCGA 192 537 462 Antisense
TAGTTTCATATCATTTGCCCTTGTC 42 655 504 Antisense
GACCTCAGCCATACCATTCAATAGC 667 381 534 Antisense
GCATATTTCTATTGGTTCACAGCAA 147 309 608 Antisense
CCATCAACAACGATCCTTATCAGTA 174 271 651 Antisense
GATTGTTAGCTTCACACATCTTCTA 87 435 720 Antisense
TTGGATGGTACCTTATTGCAACAAT 100 705 749 Antisense
GAGGCGTTCATATTACTGTGCCAAC 69 407 833 Antisense
Sequence Source Affymetrix Proprietary Database