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[183278_at]

Description C45E5.3 /REP_DB=WormBase Gene ID /WP=CE08731 /TR=Q18643 /GB=AAC24286.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 14      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 44.8
(-0.2)
A 		28.3
(-2.1)
A 		20.5
(-3)
A 		72.3
(3)
A 		52
B 24.9
(0.9)
A 		5.5
(-3)
A 		7.2
(-2.7)
A 		35.3
(3)
P 		30
C 47.5
(3)
A 		11.3
(-3)
A 		12.8
(-2.8)
A 		39.4
(1.7)
P 		36
avr 39.1
(1.3) 		15
(-2.7) 		13.5
(-3) 		49
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 25.5
(-0.4)
A 		46.1
(2.8)
A 		46.7
(2.9)
A 		8.5
(-3)
A 		24
(-0.6)
A 		47.3
(3)
A 		39
B 34.8
(1)
A 		12.3
(-3)
A 		45.9
(3)
M 		33.1
(0.7)
A 		44.8
(2.8)
P 		37.8
(1.6)
P 		34
C 29
(-3)
A 		32.9
(-1.8)
A 		41.2
(0.8)
P 		31.7
(-2.2)
A 		41.5
(0.9)
A 		48.3
(3)
P 		19
avr 29.8
(-1.4) 		30.4
(-1.2) 		44.6
(3) 		24.4
(-3) 		36.8
(0.7) 		44.5
(3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 16.3
(3)
A 		6.2
(-2.6)
A 		5.4
(-3)
A 		10.6
(-0.1)
A 		11
B 8
(3)
A 		1.3
(-3)
A 		1.7
(-2.6)
A 		7.5
(2.6)
P 		7
C 15.7
(3)
A 		3.3
(-3)
A 		4.5
(-2.4)
A 		6.6
(-1.4)
P 		12
avr 13.3
(3) 		3.6
(-3) 		3.9
(-2.8) 		8.2
(-0.2) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 4.7
(-1.9)
A 		16.4
(3)
A 		10.2
(0.4)
A 		2.1
(-3)
A 		7.2
(-0.9)
A 		11.5
(0.9)
A 		14
B 12.1
(0.4)
A 		4.1
(-3)
A 		11.2
(0)
M 		8.8
(-1)
A 		18.2
(3)
P 		14.1
(1.3)
P 		14
C 10.7
(-0.4)
A 		10.1
(-0.7)
A 		10.5
(-0.5)
P 		6.4
(-3)
A 		13.4
(1.3)
A 		16.2
(3)
P 		10
avr 9.2
(-0.5) 		10.2
(0.3) 		10.6
(0.6) 		5.8
(-3) 		12.9
(2.3) 		13.9
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183278_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10628
Sequence Type Exemplar sequence
Representative Public ID C45E5.3 NCBI
Target Description C45E5.3 /REP_DB=WormBase Gene ID /WP=CE08731 /TR=Q18643 /GB=AAC24286.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068454(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068454 NCBI Caenorhabditis elegans C45E5.3 (C45E5.3) mRNA, complete cds. 11/11 None
SNAP00000012952 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5739851:5740856:1 11/11 None
GENEFINDER00000012961 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5739562:5740856:1 11/11 None
C45E5.3 ENSEMBL cdna:known chromosome:CEL140:IV:5739812:5740856:1 gene:C45E5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5739817-5740862(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C45E5.3
Entrez Gene 183471 Entrez gene
SwissProt Q18643 EMBL-EBI
WormBase CE08731 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC24286 Hypothetical protein C45E5.3 [Caenorhabditis elegans] ref|NP_500855.1| C45E5.3 [Caenorhabditis elegans] 1.0E-154
blast AAC24286 Hypothetical protein C45E5.3 [Caenorhabditis elegans] ref|NP_500855.1| C45E5.3 [Caenorhabditis elegans] 1.0E-147
blast AAC24286 Hypothetical protein C45E5.3 [Caenorhabditis elegans] ref|NP_500855.1| C45E5.3 [Caenorhabditis elegans] 1.0E-132
blast AAC24288 Hypothetical protein C45E5.2 [Caenorhabditis elegans] ref|NP_500856.1| C45E5.2 [Caenorhabditis elegans] 3.0E-98
blast AAC24288 Hypothetical protein C45E5.2 [Caenorhabditis elegans] ref|NP_500856.1| C45E5.2 [Caenorhabditis elegans] 9.0E-95
blast AAC24288 Hypothetical protein C45E5.2 [Caenorhabditis elegans] ref|NP_500856.1| C45E5.2 [Caenorhabditis elegans] 2.0E-89
blast CAD59145 Hypothetical protein F15A4.8b [Caenorhabditis elegans] ref|NP_871952.1| F15A4.8b [Caenorhabditis elegans] 1.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 5.4E-72
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 1.1E-70
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 2.5E-62
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000012961 1 20-39
Sequence
Target Sequence 
>C. ELEGANS:183278_AT
ctgctcttcagtccgaattttgttcgttccttaaagaactgaaaaatgaacttatgaatc
aagaaaaacaatacattttgtcagttggagcccctccagctggaatagaaaacttggaag
atggatacgatatcgaagaaataattagacatgtcgattttgtcaatgtctattctatgg
attatgcgggaccatgggataatcaatggggtacacccacaggaccatctgctccattgt
acggagggcttgatgctaggaggaattttaacgtcgattatactataaaatcctatatca
gaaacattcgcagacctgaaaaattcaatttggcaattccattttcagtgaggctctgga
gaaatgtggaagatgccattcaacctggaattgaagtcttcagaaatgtcacacttcaag
atgatcgagcgatcggagaagcttcaaaagctaattatgtgaaatcaaagaatcttggag
gagtatggatttggacgatggaacatgatgatgacaatgataccttgattgaagctgtct
ccagtcaattcggaactccatgtgatgaacagatcccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGCTCTTCAGTCCGAATTTTGTTC 388 237 203 Antisense
ATACATTTTGTCAGTTGGAGCCCCT 664 19 273 Antisense
GAGCCCCTCCAGCTGGAATAGAAAA 579 383 290 Antisense
GTCGATTTTGTCAATGTCTATTCTA 562 473 355 Antisense
GGCAATTCCATTTTCAGTGAGGCTC 123 535 534 Antisense
GGAAGATGCCATTCAACCTGGAATT 615 531 570 Antisense
GTCACACTTCAAGATGATCGAGCGA 340 463 610 Antisense
GATCGAGCGATCGGAGAAGCTTCAA 650 417 625 Antisense
GATACCTTGATTGAAGCTGTCTCCA 287 425 721 Antisense
GCTGTCTCCAGTCAATTCGGAACTC 546 293 736 Antisense
ACTCCATGTGATGAACAGATCCCGT 349 99 757 Antisense
Sequence Source Affymetrix Proprietary Database