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[183270_at]

Description C14A4.12 /REP_DB=WormBase Gene ID /WP=CE02141 /TR=Q17959 /GB=CAA90116.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 81      4grp_high_8_8 > 25      6grp_highest_nonorm > 72      6grp_total > 91      4grp_total > 39      6grp_highest_sub > 05      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 266
1 2 3 4 range
A 527.6
(3)
P 		279.5
(-3)
P 		286.8
(-2.8)
A 		352.9
(-1.2)
P 		248
B 490.1
(3)
P 		363.4
(-0.5)
P 		274.7
(-3)
P 		351.2
(-0.9)
P 		215
C 540.5
(3)
P 		380.5
(-1.4)
P 		324.5
(-3)
P 		426.7
(-0.2)
P 		216
avr 519.4
(3) 		341.1
(-1.8) 		295.3
(-3) 		376.9
(-0.8) 		224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 394
1 2 3 4 5 6 range
A 513
(0.8)
P 		505.4
(0.7)
P 		333.3
(-2.2)
P 		539
(1.2)
P 		644.8
(3)
P 		282.1
(-3)
P 		363
B 442.5
(0.1)
P 		454.4
(0.3)
P 		250.4
(-3)
P 		622.1
(3)
P 		548.2
(1.8)
P 		403.9
(-0.5)
P 		372
C 361.1
(-3)
P 		448.9
(-0.4)
P 		381.6
(-2.4)
P 		566.7
(3)
P 		488.2
(0.7)
P 		436.6
(-0.8)
P 		206
avr 438.9
(-0.2) 		469.6
(0.5) 		321.8
(-3) 		575.9
(3) 		560.4
(2.6) 		374.2
(-1.8) 		254
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 192.1
(3)
P 		60.7
(-2.6)
P 		75
(-2)
A 		51.7
(-3)
P 		140
B 156.3
(3)
P 		89.8
(-1.3)
P 		63.6
(-3)
P 		74.8
(-2.3)
P 		93
C 178.9
(3)
P 		109.4
(-0.9)
P 		113.1
(-0.7)
P 		72
(-3)
P 		107
avr 175.8
(3) 		86.6
(-1.9) 		83.9
(-2) 		66.2
(-3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 94.6
(-1.8)
P 		179.8
(2.3)
P 		72.6
(-2.8)
P 		136.7
(0.2)
P 		194.6
(3)
P 		68.7
(-3)
P 		126
B 154
(0.5)
P 		151.2
(0.4)
P 		60.8
(-3)
P 		164.8
(0.9)
P 		222.7
(3)
P 		150.6
(0.3)
P 		162
C 133.9
(0.6)
P 		137
(0.9)
P 		97.1
(-3)
P 		114
(-1.3)
P 		158
(3)
P 		146.2
(1.8)
P 		61
avr 127.5
(-0.4) 		156
(1.1) 		76.8
(-3) 		138.5
(0.2) 		191.8
(3) 		121.8
(-0.7) 		115
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183270_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2815
Sequence Type Exemplar sequence
Representative Public ID C14A4.12 NCBI
Target Description C14A4.12 /REP_DB=WormBase Gene ID /WP=CE02141 /TR=Q17959 /GB=CAA90116.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026777(11), NM_001026778(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026777 NCBI Caenorhabditis elegans C14A4.12a (C14A4.12) mRNA, complete cds. 11/11 None
NM_001026778 NCBI Caenorhabditis elegans C14A4.12b (C14A4.12) mRNA, complete cds. 11/11 None
SNAP00000002494 ENSEMBL cdna:SNAP chromosome:CEL140:II:10613036:10618074:1 11/11 None
GENEFINDER00000002504 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10613105:10618074:1 11/11 None
C14A4.12a ENSEMBL cdna:known chromosome:CEL140:II:10612931:10618243:1 gene:C14A4.12 11/11 None
C14A4.12b ENSEMBL cdna:known chromosome:CEL140:II:10612931:10618074:1 gene:C14A4.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10612927-10618071(+) 100.0 100.0
Public Domain and Genome References
Ensembl C14A4.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90116 Hypothetical protein C14A4.12a [Caenorhabditis elegans] ref|NP_001021948.1| C14A4.12a [Caenorhabditis elegans] 0.0
blast CAF31470 Hypothetical protein C14A4.12b [Caenorhabditis elegans] ref|NP_001021949.1| C14A4.12b [Caenorhabditis elegans] 0.0
blast CAE59640 Hypothetical protein CBG03053 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021948.1 17 5-24,66-85,95-117,130-152,199-221,241-263,278-297,309-328,343-365,384-406,421-438,445-467,503-525,554-576,591-610,617-636,651-673
NP_001021949.1 17 5-24,66-85,95-117,130-152,179-201,221-243,258-277,289-308,323-345,364-386,401-418,425-447,483-505,534-556,571-590,597-616,631-653
C14A4.12a 17 5-24,66-85,95-117,130-152,199-221,241-263,278-297,309-328,343-365,384-406,421-438,445-467,503-525,554-576,591-610,617-636,651-673
C14A4.12b 17 5-24,66-85,95-117,130-152,179-201,221-243,258-277,289-308,323-345,364-386,401-418,425-447,483-505,534-556,571-590,597-616,631-653
SNAP00000002494 16 31-50,60-82,95-117,164-186,206-228,243-262,274-293,308-330,349-371,386-403,410-432,468-490,519-541,556-575,582-601,616-638
GENEFINDER00000002504 16 7-27,37-59,72-94,121-143,243-265,280-299,311-330,345-367,386-408,423-440,447-469,505-527,556-578,593-612,619-638,653-675
Sequence
Target Sequence 
>C. ELEGANS:183270_AT
tggagtcgtcatgttgttctctgctgttttcgtattatctcttgccttttacgtcttctt
ccgagccttcagtcctcatccactggttactatttattcacaactatttcacgttgttct
cgcactttctatcacttcatacgcgttgttccaggtgctagtcgccgagcatctctcgcg
atatccaatattctcatgtgctctagtcattctttctgtcattcgggcattggatattct
aactcaaatcgattataaagacattggaaatcaaccattgacatggacaattcatgcgtt
ggttgaatatatggctatatttttccattttgccaccattgccaccatcttcaaaattga
aggagctggaagaccactgattgagccggctgagatcccaatgtcatttgacaatcccct
gggcaatgcaacag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAGTCGTCATGTTGTTCTCTGCT 682 549 1620 Antisense
GTTCTCTGCTGTTTTCGTATTATCT 375 443 1635 Antisense
TTCAGTCCTCATCCACTGGTTACTA 650 687 1687 Antisense
CGCACTTTCTATCACTTCATACGCG 704 253 1740 Antisense
CATACGCGTTGTTCCAGGTGCTAGT 308 213 1757 Antisense
GCATCTCTCGCGATATCCAATATTC 644 309 1788 Antisense
TGCTCTAGTCATTCTTTCTGTCATT 462 581 1818 Antisense
TATATTTTTCCATTTTGCCACCATT 439 661 1935 Antisense
CACCATTGCCACCATCTTCAAAATT 590 201 1953 Antisense
CTGATTGAGCCGGCTGAGATCCCAA 429 237 1996 Antisense
GACAATCCCCTGGGCAATGCAACAG 115 365 2029 Antisense
Sequence Source Affymetrix Proprietary Database