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[183264_at]

Description F29C4.8 /REP_DB=WormBase Gene ID /WP=CE17725 /TR=O76368 /GB=AAC19194.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 80      4grp_total > 44      6grp_cgc > 95      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 22.7
(3)
A 		9.9
(-1.2)
A 		9.7
(-1.2)
A 		4.2
(-3)
A 		19
B 3.3
(-3)
A 		33.7
(3)
A 		10.5
(-1.6)
A 		6.5
(-2.4)
A 		30
C 14.9
(2.5)
A 		12.5
(1.2)
A 		4.8
(-3)
A 		15.8
(3)
A 		11
avr 13.6
(0.1) 		18.7
(3) 		8.3
(-3) 		8.8
(-2.7) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 5.3
(-1.6)
A 		9.4
(2.3)
A 		8.7
(1.6)
A 		4.1
(-2.8)
A 		10.1
(3)
A 		3.9
(-3)
A 		6
B 4.9
(-2.1)
A 		4
(-2.6)
A 		3.8
(-2.8)
A 		13
(3)
A 		5.3
(-1.8)
A 		3.4
(-3)
A 		10
C 6.1
(-2.8)
A 		3.4
(-3)
A 		3.2
(-3)
A 		80.3
(3)
A 		12.5
(-2.3)
A 		8.4
(-2.6)
A 		77
avr 5.4
(-3) 		5.6
(-2.9) 		5.2
(-3) 		32.5
(3) 		9.3
(-2.1) 		5.2
(-3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 8.3
(3)
A 		2.2
(-1.8)
A 		2.5
(-1.5)
A 		0.6
(-3)
A 		8
B 1.1
(-3)
A 		8.3
(3)
A 		2.4
(-1.9)
A 		1.4
(-2.8)
A 		7
C 4.9
(3)
A 		3.6
(0.6)
A 		1.7
(-3)
A 		2.7
(-1.1)
A 		3
avr 4.8
(3) 		4.7
(2.8) 		2.2
(-1.9) 		1.6
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 1
(-3)
A 		3.4
(3)
A 		1.9
(-0.8)
A 		1
(-3)
A 		3
(2)
A 		1
(-3)
A 		2
B 1.7
(-1.2)
A 		1.3
(-2.1)
A 		0.9
(-3)
A 		3.5
(3)
A 		2.2
(0)
A 		1.3
(-2.1)
A 		3
C 2.3
(-2.4)
A 		1
(-2.9)
A 		0.8
(-3)
A 		16.1
(3)
A 		4.1
(-1.7)
A 		2.8
(-2.2)
A 		15
avr 1.7
(-2.5) 		1.9
(-2.3) 		1.2
(-3) 		6.9
(3) 		3.1
(-1) 		1.7
(-2.5) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183264_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10902
Sequence Type Exemplar sequence
Representative Public ID F29C4.8 NCBI
Target Description F29C4.8 /REP_DB=WormBase Gene ID /WP=CE17725 /TR=O76368 /GB=AAC19194.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067468(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067468 NCBI Caenorhabditis elegans COLlagen family member (col-99) (col-99) mRNA, complete cds. 10/11 None
SNAP00000028014 ENSEMBL cdna:SNAP chromosome:CEL140:IV:138760:142521:-1 10/11 None
GENEFINDER00000028017 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:138699:144886:-1 10/11 None
F29C4.8 ENSEMBL cdna:known chromosome:CEL140:IV:138461:144950:-1 gene:F29C4.8 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:138429-144887(-) 99.89 99.89
Public Domain and Genome References
Gene Title
Gene Symbol col-99
Ensembl F29C4.8
Entrez Gene 185112 Entrez gene
SwissProt O76368 EMBL-EBI
WormBase CE34300 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6817 phosphate transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC19194 Collagen protein 99 [Caenorhabditis elegans] ref|NP_499869.2| COLlagen family member (col-99) [Caenorhabditis elegans] 0.0
blast CAE58502 Hypothetical protein CBG01652 [Caenorhabditis briggsae] 1.0E-142
blast AAC19194 Collagen protein 99 [Caenorhabditis elegans] ref|NP_499869.2| COLlagen family member (col-99) [Caenorhabditis elegans] 1.0E-132
blast CAE58502 Hypothetical protein CBG01652 [Caenorhabditis briggsae] 1.0E-110
blast AAC19194 Collagen protein 99 [Caenorhabditis elegans] ref|NP_499869.2| COLlagen family member (col-99) [Caenorhabditis elegans] 1.0E-103
blast CAE58502 Hypothetical protein CBG01652 [Caenorhabditis briggsae] 4.0E-82
blast AAO39561 LP07855p [Drosophila melanogaster] 7.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.3E-4
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 9.6E-7
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 3.7E-4
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.3E-5
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.8E-5
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 8.9E-6
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.1E-8
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.9E-5
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 4.2E-12
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 2.0E-9
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 3.7E-4
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.3E-5
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.8E-5
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 8.9E-6
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.1E-8
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 5.4E-11
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.3E-4
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 9.6E-7
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 3.7E-4
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.3E-5
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.8E-5
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 8.9E-6
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.1E-8
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 5.4E-11
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000028017 1 88-105
Sequence
Target Sequence 
>C. ELEGANS:183264_AT
acctaggggacatcacggaccagtagggccgtcaggagaacgtggacctcagggtcttcc
aggacattccggagagcgcggagatcgaggagacatcggaccgcccggattgccagggca
acctggcgccggagagatcagcggcagtcagtcaggcccacggggtccacctggccttcc
aggaccgcctggagaaaagggtgatcttggacctccagggcttccaggccagcctggatc
cctgggtctcccaggtccaccaggcccgatgggtcttcgaggccctcacggcacagaggg
agaaacgggaaaacaagggcccgaaggatcgaaaggatatccggggcctatgggtccaca
aggtccgcccggcaacgatggggagccaggaatcgacggaaggcctggtccagctggaga
gaagggagatcagggaatacctggactcgatgcaccatgtcctactgggccagatgggtt
acctctgccttattgttcttggaaacctatggatcgtcacaatctcgttatttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCTAGGGGACATCACGGACCAGTA 317 85 1287 Antisense
AGAACGTGGACCTCAGGGTCTTCCA 454 63 1323 Antisense
GGTCTTCCAGGACATTCCGGAGAGC 315 503 1339 Antisense
GAGATCGAGGAGACATCGGACCGCC 351 395 1367 Antisense
AGAGATCAGCGGCAGTCAGTCAGGC 91 69 1419 Antisense
AAAAGGGTGATCTTGGACCTCCAGG 234 133 1481 Antisense
AGGAATCGACGGAAGGCCTGGTCCA 227 55 1674 Antisense
AGGGAATACCTGGACTCGATGCACC 133 59 1718 Antisense
CTACTGGGCCAGATGGGTTACCTCT 113 225 1748 Antisense
TTACCTCTGCCTTATTGTTCTTGGA 490 681 1765 Antisense
GGATCGTCACAATCTCGTTATTTCT 149 513 1797 Antisense
Sequence Source Affymetrix Proprietary Database