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[183252_at]

Description K02F2.5 /REP_DB=WormBase Gene ID /WP=CE17157 /TR=O44983 /GB=AAB97563.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 12      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 31      6grp_total > 23      4grp_total > 30      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1412
1 2 3 4 range
A 1841.8
(3)
P 		666.7
(-2.5)
P 		571.4
(-3)
P 		1238.1
(0.1)
P 		1270
B 1766.1
(3)
P 		851.4
(-1.8)
P 		624.3
(-3)
P 		1549.1
(1.9)
P 		1142
C 1983.7
(3)
P 		849.7
(-2)
P 		613
(-3)
P 		1339.6
(0.2)
P 		1371
avr 1863.9
(3) 		789.3
(-2.1) 		602.9
(-3) 		1375.6
(0.7) 		1261

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 685
1 2 3 4 5 6 range
A 1057.5
(2.1)
P 		1138.3
(3)
P 		1083.9
(2.4)
P 		996.6
(1.4)
P 		677.1
(-2.3)
P 		611.6
(-3)
P 		527
B 1296.6
(3)
P 		1097.4
(0.8)
P 		1143.5
(1.3)
P 		850.7
(-1.9)
P 		745.8
(-3)
P 		794.5
(-2.5)
P 		551
C 1169.5
(3)
P 		1117.9
(2.3)
P 		1052.2
(1.4)
P 		997.1
(0.6)
P 		742.1
(-3)
P 		802.3
(-2.2)
P 		427
avr 1174.5
(3) 		1117.9
(2.3) 		1093.2
(1.9) 		948.1
(0) 		721.7
(-3) 		736.1
(-2.8) 		453
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 526
1 2 3 4 range
A 670.6
(3)
P 		144.7
(-3)
P 		149.4
(-2.9)
P 		181.2
(-2.6)
P 		526
B 563.2
(3)
P 		210.3
(-2.1)
P 		144.7
(-3)
P 		330.1
(-0.3)
P 		419
C 656.7
(3)
P 		244.3
(-2.6)
P 		213.6
(-3)
P 		225.9
(-2.8)
P 		443
avr 630.2
(3) 		199.8
(-2.6) 		169.2
(-3) 		245.7
(-2) 		461

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 302
1 2 3 4 5 6 range
A 194.9
(-1.9)
P 		404.8
(3)
P 		236.1
(-1)
P 		252.8
(-0.6)
P 		204.3
(-1.7)
P 		148.9
(-3)
P 		256
B 451.2
(3)
P 		365.3
(0.7)
P 		277.7
(-1.6)
P 		225.3
(-3)
P 		303
(-0.9)
P 		296.3
(-1.1)
P 		226
C 433.5
(3)
P 		341.1
(0.6)
P 		267.8
(-1.3)
P 		200.5
(-3)
P 		240.1
(-2)
P 		268.6
(-1.2)
P 		233
avr 359.9
(2.6) 		370.4
(3) 		260.5
(-1.6) 		226.2
(-3) 		249.1
(-2) 		237.9
(-2.5) 		144
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183252_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1947
Sequence Type Exemplar sequence
Representative Public ID K02F2.5 NCBI
Target Description K02F2.5 /REP_DB=WormBase Gene ID /WP=CE17157 /TR=O44983 /GB=AAB97563.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059550(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059550 NCBI Caenorhabditis elegans K02F2.5 (K02F2.5) mRNA, complete cds. 9/11 A 
GENEFINDER00000018483 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6813199:6818441:-1 9/11 None
K02F2.5 ENSEMBL cdna:known chromosome:CEL140:I:6812921:6818511:-1 gene:K02F2.5 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000018471 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6814906-6818372(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K02F2.5
Entrez Gene 183992 Entrez gene
SwissProt O44983 EMBL-EBI
WormBase CE31353 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB97563 Hypothetical protein K02F2.5 [Caenorhabditis elegans] ref|NP_491951.2| K02F2.5 [Caenorhabditis elegans] 7.0E-77
blast CAE67308 Hypothetical protein CBG12763 [Caenorhabditis briggsae] 3.0E-66
blast T33802 hypothetical protein E02D9.2 - Caenorhabditis elegans 2.0E-26
Sequence
Target Sequence 
>C. ELEGANS:183252_AT
attgtaacacagtcgctacttgccgttgcacttttatcagttgcaatggttgaggctgct
ccattggtaaaagtcgacaaaaaaatcaatatcttaatttgccaatttaccaaaacattg
ttcggaatttttggagagtccacaaaaggagcaacagtaaaaagcacaaaatcatcgagt
tccaagacaaaaggatacgtgccaacaccaaaagctcttcgacgtggaaaacgaaacgtg
attctcgacgaaattcccgatgattcgtctgttctgggaacagaagaagatgtgctccgt
gag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGTAACACAGTCGCTACTTGCCG 413 5 28 Antisense
AGTTGCAATGGTTGAGGCTGCTCCA 155 65 66 Antisense
GAGGCTGCTCCATTGGTAAAAGTCG 62 407 79 Antisense
ATATCTTAATTTGCCAATTTACCAA 220 15 116 Antisense
GCACAAAATCATCGAGTTCCAAGAC 477 317 191 Antisense
AAGGATACGTGCCAACACCAAAAGC 252 159 218 Antisense
CACCAAAAGCTCTTCGACGTGGAAA 508 199 233 Antisense
AACGAAACGTGATTCTCGACGAAAT 208 145 257 Antisense
GAAATTCCCGATGATTCGTCTGTTC 171 363 277 Antisense
CGATGATTCGTCTGTTCTGGGAACA 453 249 285 Antisense
AACAGAAGAAGATGTGCTCCGTGAG 117 135 306 Antisense
Sequence Source Affymetrix Proprietary Database