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[183251_s_at]

Description F31D5.4 /REP_DB=WormBase Gene ID /WP=CE28228 /TR=Q19930 /GB=AAC71100.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 36      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 80      6grp_total > 26      4grp_total > 47      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 357
1 2 3 4 range
A 464.4
(3)
P 		182.8
(-2.3)
P 		143.4
(-3)
P 		158.6
(-2.7)
P 		321
B 400.3
(3)
P 		204.9
(-1)
P 		107.3
(-3)
P 		128.7
(-2.6)
P 		293
C 463.4
(3)
P 		154
(-3)
P 		171.4
(-2.7)
P 		190
(-2.3)
P 		309
avr 442.7
(3) 		180.6
(-2.2) 		140.7
(-3) 		159.1
(-2.6) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 274
1 2 3 4 5 6 range
A 373.7
(3)
P 		245.4
(0.2)
P 		143.7
(-2.1)
P 		115.5
(-2.7)
P 		102
(-3)
P 		204
(-0.7)
P 		272
B 225.9
(1.8)
P 		256.6
(3)
P 		239.3
(2.3)
P 		178.8
(0)
P 		100.2
(-3)
P 		182.4
(0.2)
P 		156
C 284.6
(3)
P 		242.9
(1.5)
P 		163.8
(-1.3)
P 		117.7
(-3)
P 		144.7
(-2)
P 		173.6
(-1)
P 		167
avr 294.7
(3) 		248.3
(1.4) 		182.3
(-0.8) 		137.3
(-2.3) 		115.6
(-3) 		186.7
(-0.6) 		179
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 146
1 2 3 4 range
A 169.1
(3)
P 		39.7
(-2.3)
P 		37.5
(-2.4)
P 		23.2
(-3)
P 		146
B 127.7
(3)
P 		50.6
(-1.5)
P 		24.9
(-3)
P 		27.4
(-2.9)
P 		103
C 153.4
(3)
P 		44.3
(-2.4)
P 		59.7
(-1.6)
P 		32
(-3)
P 		121
avr 150.1
(3) 		44.9
(-2.1) 		40.7
(-2.4) 		27.5
(-3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 68.9
(1.1)
P 		87.3
(3)
P 		31.3
(-2.8)
P 		29.3
(-3)
P 		30.8
(-2.8)
P 		49.7
(-0.9)
P 		58
B 78.6
(2.1)
P 		85.4
(3)
P 		58.1
(-0.7)
P 		47.4
(-2.1)
P 		40.7
(-3)
P 		68
(0.7)
P 		45
C 105.5
(3)
P 		74.1
(0.7)
P 		41.7
(-1.7)
P 		23.7
(-3)
P 		46.8
(-1.3)
P 		58.1
(-0.5)
P 		82
avr 84.3
(3) 		82.3
(2.8) 		43.7
(-1.8) 		33.5
(-3) 		39.4
(-2.3) 		58.6
(-0) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183251_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4580
Sequence Type Exemplar sequence
Representative Public ID F31D5.4 NCBI
Target Description F31D5.4 /REP_DB=WormBase Gene ID /WP=CE28228 /TR=Q19930 /GB=AAC71100.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062337(11), AY314775(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062337 NCBI Caenorhabditis elegans F31D5.4 (F31D5.4) mRNA, complete cds. 11/11 None
AY314775 NCBI Caenorhabditis elegans methuselah-like protein MTH-2 mRNA, complete cds. 11/11 None
SNAP00000018588 ENSEMBL cdna:SNAP chromosome:CEL140:II:4189030:4194063:-1 10/11 None
GENEFINDER00000018599 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4189143:4194183:-1 10/11 None
F31D5.4 ENSEMBL cdna:known chromosome:CEL140:II:4188986:4194452:-1 gene:F31D5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4189028-4194451(-) 92.74 100.0
Public Domain and Genome References
Gene Title
Ensembl F31D5.4
Entrez Gene 173752 Entrez gene
SwissProt Q19930 EMBL-EBI
WormBase CE37367 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7218 neuropeptide signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAM98026 Hypothetical protein F31D5.4 [Caenorhabditis elegans] ref|NP_494738.3| F31D5.4 [Caenorhabditis elegans] 0.0
blast AAQ84882 methuselah-like protein MTH-2 [Caenorhabditis elegans] 0.0
blast CAE74280 Hypothetical protein CBG21976 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000203 EMBL-EBI GPS domain 6.4E-18
Pfam IPR000203 EMBL-EBI GPS domain 6.4E-18
Pfam IPR000203 EMBL-EBI GPS domain 6.4E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494738.3 7 689-711,732-751,766-788,801-823,846-868,889-911,921-943
AAQ84882.1 8 5-27,671-693,714-733,748-770,783-805,828-850,871-893,903-925
F31D5.4 7 689-711,732-751,766-788,801-823,846-868,889-911,921-943
SNAP00000018588 7 580-602,623-642,657-679,692-714,729-748,755-774,784-806
GENEFINDER00000018599 8 5-27,671-693,714-733,748-770,783-805,828-850,871-893,903-925
Sequence
Target Sequence 
>C. ELEGANS:183251_S_AT
ttcgaaatatcctcgttttcctgccaactccagcctacaggctgcttaacatatgttttg
gaacagtgccagttctcatcacgtcagtagctctcccctttattctttcaatatttctgc
tagtcttcacaaaattcttcgaccgccaggactgcttctgctgggtacgacccgactaca
tagtggctgcaattatcattccagtaactgtacttttactgagtacggtagtatgcacta
gtttcatgatctacaaagttttttgtggaatgaaaaggaaatttagtcaagggggtcacc
actacgatccaaatgtgatcacgaagatagtatccatagttgtaatgcaaatctcactag
gtcttccatggatccttcaattcggaaccctctactccccatacaccaccttctggcact
acaccttcaccatagtcttagggtctcagggaacaattttactgctcattttcctctaca
agcgatatcgagcttggcaagctgatagtacgctcaagtctcgaggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGAAATATCCTCGTTTTCCTGCC 368 693 1790 Antisense
TGGAACAGTGCCAGTTCTCATCACG 61 547 1848 Antisense
TCTCCCCTTTATTCTTTCAATATTT 466 611 1881 Antisense
GGTACGACCCGACTACATAGTGGCT 297 503 1953 Antisense
AGTCAAGGGGGTCACCACTACGATC 97 59 2074 Antisense
TGCAAATCTCACTAGGTCTTCCATG 306 577 2135 Antisense
ATGGATCCTTCAATTCGGAACCCTC 6 53 2157 Antisense
ACACCTTCACCATAGTCTTAGGGTC 265 105 2210 Antisense
ACAATTTTACTGCTCATTTTCCTCT 143 113 2242 Antisense
CATTTTCCTCTACAAGCGATATCGA 699 215 2256 Antisense
GATAGTACGCTCAAGTCTCGAGGAC 115 423 2293 Antisense
Sequence Source Affymetrix Proprietary Database