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[183250_at]

Description ZC513.5 /REP_DB=WormBase Gene ID /WP=CE28166 /TR=Q23361 /GB=AAC48267.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      6grp_highest_nonorm > 81      6grp_total > 80      4grp_total > 60      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 235.9
(2.3)
P 		198.2
(0.7)
P 		252.5
(3)
P 		111.9
(-3)
A 		141
B 276.8
(2.5)
P 		263.6
(2)
P 		291.8
(3)
P 		127.3
(-3)
A 		165
C 289.2
(3)
P 		272.6
(2.4)
P 		274.1
(2.4)
P 		129
(-3)
A 		160
avr 267.3
(2.8) 		244.8
(1.9) 		272.8
(3) 		122.7
(-3) 		150

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 205.6
(-3)
P 		281.4
(1.7)
P 		249.5
(-0.3)
P 		298.8
(2.8)
P 		302.7
(3)
P 		234.1
(-1.2)
P 		97
B 263.7
(-0.8)
P 		236.6
(-3)
P 		301.5
(2.3)
P 		310.2
(3)
P 		248
(-2.1)
P 		262.5
(-0.9)
P 		74
C 323.5
(3)
P 		282.4
(0.8)
P 		223.7
(-2.3)
P 		317.8
(2.7)
P 		210.1
(-3)
P 		256.7
(-0.5)
P 		113
avr 264.3
(-1.6) 		266.8
(-1.4) 		258.2
(-2.3) 		308.9
(3) 		253.6
(-2.7) 		251.1
(-3) 		58
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 85.9
(3)
P 		43
(-0.7)
P 		66
(1.3)
P 		16.4
(-3)
A 		70
B 88.3
(3)
P 		65.1
(0.7)
P 		67.6
(1)
P 		27.1
(-3)
A 		61
C 95.7
(3)
P 		78.4
(1.6)
P 		95.5
(3)
P 		21.8
(-3)
A 		74
avr 90
(3) 		62.2
(0.6) 		76.4
(1.8) 		21.8
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 37.9
(-3)
P 		100.1
(3)
P 		54.3
(-1.4)
P 		75.8
(0.7)
P 		91.4
(2.2)
P 		57
(-1.2)
P 		62
B 91.8
(1)
P 		78.8
(-1.8)
P 		73.2
(-3)
P 		82.2
(-1)
P 		100.8
(3)
P 		97.9
(2.4)
P 		28
C 119.9
(3)
P 		86.2
(-0.2)
P 		56.9
(-3)
P 		63.9
(-2.3)
P 		68
(-1.9)
P 		85.9
(-0.2)
P 		63
avr 83.2
(1.8) 		88.4
(3) 		61.5
(-3) 		74
(-0.2) 		86.7
(2.6) 		80.3
(1.2) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183250_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12104
Sequence Type Exemplar sequence
Representative Public ID ZC513.5 NCBI
Target Description ZC513.5 /REP_DB=WormBase Gene ID /WP=CE28166 /TR=Q23361 /GB=AAC48267.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072670(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072670 NCBI Caenorhabditis elegans ZC513.5 (ZC513.5) mRNA, complete cds. 11/11 None
SNAP00000025309 ENSEMBL cdna:SNAP chromosome:CEL140:V:8037703:8041848:1 11/11 None
GENEFINDER00000025322 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8037703:8052119:1 11/11 None
ZC513.5 ENSEMBL cdna:known chromosome:CEL140:V:8037702:8042097:1 gene:ZC513.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8038805-8041057(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZC513.5
Entrez Gene 179185 Entrez gene
SwissProt Q23361 EMBL-EBI
WormBase CE29152 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1603523_AT similar to alpha-1,6-mannosyltransferase ALG12 cfa
CANINE_2:CFA.14439.1.S1_AT similar to alpha-1,6-mannosyltransferase ALG12 cfa
CANINE_2:CFA.14439.1.S1_S_AT similar to alpha-1,6-mannosyltransferase ALG12 cfa
CANINE_2:CFAAFFX.1995.1.S1_AT similar to alpha-1,6-mannosyltransferase ALG12 cfa
CANINE_2:CFAAFFX.1996.1.S1_AT similar to alpha-1,6-mannosyltransferase ALG12 cfa
DROSOPHILA_2:1634528_AT dm
DROSGENOME1:149581_AT dm
CHICKEN:GGAAFFX.11495.1.S1_S_AT similar to dolichyl-P-mannose:Man7GlcNAc2-PP-dolichyl mannosyltransferase; alpha-1,6-mannosyltransferase homolog; dolichyl-P-mannose:Man-7-GlcNAc-2-PP-dolichyl-alpha-6-mannosyltransferase gga
CHICKEN:GGAAFFX.22619.1.S1_AT similar to dolichyl-P-mannose:Man7GlcNAc2-PP-dolichyl mannosyltransferase; alpha-1,6-mannosyltransferase homolog; dolichyl-P-mannose:Man-7-GlcNAc-2-PP-dolichyl-alpha-6-mannosyltransferase gga
HG-U133_PLUS_2:218444_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) hs
HG-U133A:218444_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) hs
HG-U133A_2:218444_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) hs
HU35KSUBC:RC_R52649_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) hs
U133_X3P:G13129113_3P_X_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) hs
U133_X3P:G13129113_3P_S_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) hs
U133_X3P:G13129113_3P_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) hs
U133_X3P:218444_3P_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) hs
HG-U95B:54946_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) hs
RAE230A:1372902_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) (predicted) rn
RAT230_2:1372902_AT asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) (predicted) rn
RAE230B:1380740_AT Asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) (predicted) rn
RAT230_2:1380740_AT Asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAC48267 Hypothetical protein ZC513.5 [Caenorhabditis elegans] ref|NP_505071.1| ZC513.5 [Caenorhabditis elegans] sp|Q23361|ALG12_CAEEL Probable dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1, 6-mannosyltransferase (Mannosyltransferase ALG12 homolog) 0.0
blast AAC48265 Hypothetical protein ZC513.3 [Caenorhabditis elegans] ref|NP_505073.1| ZC513.3 [Caenorhabditis elegans] 0.0
blast CAE66270 Hypothetical protein CBG11514 [Caenorhabditis briggsae] 1.0E-179
blast CAE66152 Hypothetical protein CBG11382 [Caenorhabditis briggsae] 1.0E-168
blast EAA06341 ENSANGP00000019882 [Anopheles gambiae str. PEST] ref|XP_311046.2| ENSANGP00000019882 [Anopheles gambiae str. PEST] 3.0E-89
Protein Domains
Database ID Description E-Value
Pfam IPR005599 EMBL-EBI Plasmid Maintenance Protein 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505071.1 10 7-24,59-81,88-110,130-152,172-194,198-220,255-274,284-301,308-330,340-362
ZC513.5 10 7-24,59-81,88-110,130-152,172-194,198-220,255-274,284-301,308-330,340-362
SNAP00000025309 10 7-24,59-81,88-110,130-152,172-194,198-220,255-274,284-301,308-330,340-362
GENEFINDER00000025322 10 60-82,88-110,130-152,172-194,206-228,257-279,284-301,306-328,340-362,1078-1100
Sequence
Target Sequence 
>C. ELEGANS:183250_AT
gcaatgtgtctagctgtctccattccaatcgattcctatttttggggacgacctctttgg
ccagaaggagaagtcatgttcttcaatgtcgttgagaacagaagccatgaatatggtacg
caaccattcttatggtatttctattctgctcttccacgatgtctactcaccactacattg
cttgttcctctgggtcttttggtagatcgcagacttccacaaattgtacttccaagtgtc
atcttcatattcctctacagtttcttaccacacaaagaactccgatttatcatctacgtt
ctgccaatcttctgcctttcagcggcagttttctgtgctagaatgctcattaacagacat
aagtcgttcttccgtatgattctatttttcggagtcatccttcatttactcgccaatgtt
ctatgcactggaatgttcttgcttgttgctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAATGTGTCTAGCTGTCTCCATTC 121 323 523 Antisense
ATGGTATTTCTATTCTGCTCTTCCA 571 51 654 Antisense
TACTCACCACTACATTGCTTGTTCC 63 647 686 Antisense
GTTCCTCTGGGTCTTTTGGTAGATC 289 441 706 Antisense
AAGTGTCATCTTCATATTCCTCTAC 466 159 756 Antisense
CGATTTATCATCTACGTTCTGCCAA 146 249 805 Antisense
GCCTTTCAGCGGCAGTTTTCTGTGC 322 281 836 Antisense
AAGTCGTTCTTCCGTATGATTCTAT 369 157 883 Antisense
TCGGAGTCATCCTTCATTTACTCGC 353 607 911 Antisense
TTACTCGCCAATGTTCTATGCACTG 302 683 928 Antisense
ACTGGAATGTTCTTGCTTGTTGCTT 374 97 949 Antisense
Sequence Source Affymetrix Proprietary Database