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[183231_at]

Description K01A2.3 /REP_DB=WormBase Gene ID /WP=CE21010 /TR=Q9TZ53 /GB=AAC69512.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 12      6grp_highest_nonorm > 51      6grp_total > 22      4grp_total > 54      6grp_highest_sub > 02      6grp_high1_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1097
1 2 3 4 range
A 1113.8
(3)
P 		357.9
(-1.2)
P 		248.3
(-1.8)
P 		40.3
(-3)
A 		1074
B 711.1
(3)
P 		631.8
(2.3)
P 		313
(-0.4)
P 		16.5
(-3)
A 		695
C 747.5
(3)
P 		439.4
(0.4)
P 		689.5
(2.5)
P 		43.6
(-3)
A 		704
avr 857.5
(3) 		476.4
(0.2) 		416.9
(-0.2) 		33.5
(-3) 		824

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 652
1 2 3 4 5 6 range
A 470.4
(0)
P 		789.1
(3)
P 		646.5
(1.7)
P 		498.5
(0.3)
P 		319.7
(-1.4)
P 		148
(-3)
P 		641
B 676.3
(2.6)
P 		711
(3)
P 		619.9
(2)
P 		318.2
(-1.1)
P 		163.2
(-2.7)
P 		136.8
(-3)
P 		574
C 782.3
(3)
P 		762.2
(2.8)
P 		719.7
(2.4)
P 		539.5
(0.6)
P 		268.9
(-2.1)
P 		179.9
(-3)
P 		602
avr 643
(1.9) 		754.1
(3) 		662
(2.1) 		452.1
(-0) 		250.6
(-2) 		154.9
(-3) 		599
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 402
1 2 3 4 range
A 405.5
(3)
P 		77.7
(-1.9)
P 		64.9
(-2.1)
P 		5.9
(-3)
A 		400
B 226.8
(3)
P 		156.1
(1.1)
P 		72.5
(-1.1)
P 		3.5
(-3)
A 		223
C 247.5
(3)
P 		126.3
(-0)
P 		240.3
(2.8)
P 		7.3
(-3)
A 		240
avr 293.3
(3) 		120
(-0.6) 		125.9
(-0.5) 		5.6
(-3) 		288

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 86.7
(-1.8)
P 		280.6
(3)
P 		140.8
(-0.4)
P 		126.4
(-0.8)
P 		96.5
(-1.5)
P 		36
(-3)
P 		245
B 235.3
(3)
P 		236.7
(3)
P 		150.5
(0.2)
P 		84.3
(-1.9)
P 		66.3
(-2.5)
P 		51
(-3)
P 		186
C 290
(3)
P 		232.6
(1.5)
P 		183.2
(0.2)
P 		108.5
(-1.7)
P 		87
(-2.3)
P 		60.2
(-3)
P 		230
avr 204
(1.6) 		250
(3) 		158.2
(0.3) 		106.4
(-1.3) 		83.3
(-2) 		49.1
(-3) 		201
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183231_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4886
Sequence Type Exemplar sequence
Representative Public ID K01A2.3 NCBI
Target Description K01A2.3 /REP_DB=WormBase Gene ID /WP=CE21010 /TR=Q9TZ53 /GB=AAC69512.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027056(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027056 NCBI Caenorhabditis elegans K01A2.3 (K01A2.3) mRNA, complete cds. 10/11 None
SNAP00000016836 ENSEMBL cdna:SNAP chromosome:CEL140:II:315158:315783:1 10/11 None
K01A2.3 ENSEMBL cdna:known chromosome:CEL140:II:315049:315783:1 gene:K01A2.3 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000016834 7/11 Cross Hyb Matching Probes None
GENEFINDER00000016843 3/11 Cross Hyb Matching Probes None
GENEFINDER00000016845 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:315025-315783(+) 100.0 100.0
chrII:308689-309448(+) 98.44 98.44
Public Domain and Genome References
Gene Title
Ensembl K01A2.3
Entrez Gene 173420 Entrez gene
SwissProt Q7KNR0 EMBL-EBI
WormBase CE29422 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR12969 Hypothetical protein K01A2.3 [Caenorhabditis elegans] ref|NP_001022227.1| K01A2.3 [Caenorhabditis elegans] 6.0E-45
blast T33645 hypothetical protein K01A2.3 - Caenorhabditis elegans 6.0E-45
blast AAR12970 Hypothetical protein K01A2.4 [Caenorhabditis elegans] 2.0E-43
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022227.1 3 22-44,57-76,91-113
K01A2.3 3 22-44,57-76,91-113
SNAP00000016836 3 22-44,57-76,91-113
Sequence
Target Sequence 
>C. ELEGANS:183231_AT
tcccgatgtgaaagagtggcaaaaaaagaaactcctcattggacacggtatctcgttttt
aattcatgtcattatgacctataccttgtttcttgaatggtacaatgcaatcgatccaaa
aattactgtagttggagctattattctgatggttttcgtcttgtgtttattacttctcgc
gttcggcggcttcgtctggatgttgcacggaaaatataaaaacgaaaaggcagagacgga
gtttttcattttactccccctgttcaacatcgcgtgtgcaacaatctatcagtttgttgt
tcaacttttggcatctcaattgtcaataaaagtaccaaatctggcgtcctcaattgcggc
ctctatatcagttgcaactcatatgattcttcatacttttgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCCGATGTGAAAGAGTGGCAAAAA 499 597 30 Antisense
CCTGTTCAACATCGCGTGTGCAACA 273 267 288 Antisense
ATCGCGTGTGCAACAATCTATCAGT 76 37 298 Antisense
ACAATCTATCAGTTTGTTGTTCAAC 65 115 310 Antisense
GTTGTTCAACTTTTGGCATCTCAAT 696 437 325 Antisense
ACTTTTGGCATCTCAATTGTCAATA 429 101 333 Antisense
AAGTACCAAATCTGGCGTCCTCAAT 324 157 359 Antisense
CCTCAATTGCGGCCTCTATATCAGT 278 269 377 Antisense
GCGGCCTCTATATCAGTTGCAACTC 672 293 385 Antisense
AGTTGCAACTCATATGATTCTTCAT 257 65 399 Antisense
TCATATGATTCTTCATACTTTTGCA 490 619 408 Antisense
Sequence Source Affymetrix Proprietary Database