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[183227_at]

Description T07A9.4 /REP_DB=WormBase Gene ID /WP=CE17213 /TR=O44407 /GB=AAK39277.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 86      4grp_high_8_8 > 57      6grp_highest_nonorm > 91      6grp_total > 78      4grp_total > 61      6grp_highest_sub > In      6grp_cgc > 59      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 142
(-3)
P 		142.5
(-2.8)
P 		157.7
(3)
P 		146.3
(-1.4)
M 		16
B 168.8
(3)
P 		158.8
(0.7)
A 		144.5
(-2.5)
M 		142.5
(-3)
P 		26
C 148.1
(2.3)
P 		151.8
(3)
P 		145.7
(1.9)
P 		118.4
(-3)
M 		33
avr 153
(3) 		151
(2.3) 		149.3
(1.7) 		135.7
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 239
1 2 3 4 5 6 range
A 159.7
(-3)
P 		197.9
(-1.9)
P 		191.4
(-2)
P 		190
(-2.1)
P 		255.4
(-0.1)
P 		359.6
(3)
P 		200
B 178.8
(-2.1)
P 		162.9
(-2.5)
P 		206.3
(-1.4)
P 		145.5
(-3)
P 		244.1
(-0.4)
P 		371.9
(3)
P 		226
C 164.8
(-2.4)
P 		140.5
(-3)
P 		236.6
(-0.6)
P 		159.7
(-2.5)
P 		247
(-0.3)
P 		379.9
(3)
P 		239
avr 167.8
(-2.9) 		167.1
(-2.9) 		211.4
(-1.6) 		165.1
(-3) 		248.8
(-0.6) 		370.5
(3) 		205
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 51.7
(3)
P 		30.9
(-1.1)
P 		41.2
(0.9)
P 		21.4
(-3)
M 		30
B 53.8
(3)
P 		39.2
(-0.7)
A 		33.5
(-2.2)
M 		30.4
(-3)
P 		23
C 49
(2.6)
P 		43.6
(1.6)
P 		50.8
(3)
P 		20
(-3)
M 		31
avr 51.5
(3) 		37.9
(0) 		41.8
(0.9) 		23.9
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 109
1 2 3 4 5 6 range
A 29.4
(-3)
P 		70.4
(1.2)
P 		41.7
(-1.7)
P 		48.2
(-1.1)
P 		77.1
(1.9)
P 		87.6
(3)
P 		58
B 62.2
(-1.6)
P 		54.2
(-2.1)
P 		50.1
(-2.3)
P 		38.5
(-3)
P 		99.2
(0.6)
P 		138.7
(3)
P 		100
C 61.1
(-1.2)
P 		42.9
(-2.3)
P 		60.2
(-1.2)
P 		32.1
(-3)
P 		79.9
(0)
P 		127.2
(3)
P 		95
avr 50.9
(-2.1) 		55.8
(-1.8) 		50.7
(-2.1) 		39.6
(-3) 		85.4
(0.5) 		117.8
(3) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183227_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10967
Sequence Type Exemplar sequence
Representative Public ID T07A9.4 NCBI
Target Description T07A9.4 /REP_DB=WormBase Gene ID /WP=CE17213 /TR=O44407 /GB=AAK39277.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171244(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171244 NCBI Caenorhabditis elegans Enhanced RNAI (RNA interference) family member (eri-1) (eri-1) mRNA, complete cds. 11/11 A 
SNAP00000017767 ENSEMBL cdna:SNAP chromosome:CEL140:IV:415777:419951:-1 11/11 None
GENEFINDER00000017774 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:415777:419951:-1 11/11 None
T07A9.5b ENSEMBL cdna:known chromosome:CEL140:IV:415654:419951:-1 gene:T07A9.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176868 2 1 NM_171244 183227_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:415776-416803(-) 100.0 100.0
Public Domain and Genome References
Ensembl T07A9.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39277 Enhanced rnai (rna interference) protein 1, isoform b [Caenorhabditis elegans] ref|NP_741292.1| Enhanced RNAI (RNA interference) family member (eri-1) [Caenorhabditis elegans] sp|O44406|ERI1_CAEEL 3'-5' exonuclease eri-1 (Enhanced RNAi protein) 0.0
blast AAK39278 Enhanced rnai (rna interference) protein 1, isoform a [Caenorhabditis elegans] ref|NP_741293.1| Enhanced RNAI (RNA interference) family member (eri-1) [Caenorhabditis elegans] 0.0
blast CAE67939 Hypothetical protein CBG13539 [Caenorhabditis briggsae] 1.0E-179
Protein Domains
Database ID Description E-Value
Pfam IPR006055 EMBL-EBI Exonuclease 1.2E-46
Pfam IPR003034 EMBL-EBI DNA-binding SAP 1.9E-8
Sequence
Target Sequence 
>C. ELEGANS:183227_AT
gatttcgcgaaaattgctgctgagcggggaatttttaaaattggtgaaataaaatcatat
caaactgcaagaccgataatagaggatgatgacgtggatgttgaaagtgaagaggaagat
tatggaactgaatttgaaatgttggaagttgtggaaagaatgcctccagttagttctacg
ttacacactgaagtcgatttagatgctgtatgggaacgagatggtggaagtgattctgaa
agagaaaacctctcaaatgctccaagtctccacgagtttccatcgtcatccacatcatca
ccacatgccacgtcagaacatgtgacgtcatcatcaccacttcatatcgacgatgacgtg
gaccgcgtgttgaatgcgccgcccaaaaattcgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTTCGCGAAAATTGCTGCTGAGC 508 427 52 Antisense
TGCTGCTGAGCGGGGAATTTTTAAA 226 581 66 Antisense
AATGCCTCCAGTTAGTTCTACGTTA 25 167 210 Antisense
GTTCTACGTTACACACTGAAGTCGA 686 433 224 Antisense
GAAGTCGATTTAGATGCTGTATGGG 704 335 241 Antisense
GAAAACCTCTCAAATGCTCCAAGTC 146 355 295 Antisense
AAATGCTCCAAGTCTCCACGAGTTT 697 119 306 Antisense
TCACCACATGCCACGTCAGAACATG 563 625 349 Antisense
TCATCATCACCACTTCATATCGACG 354 619 379 Antisense
ACGATGACGTGGACCGCGTGTTGAA 606 93 401 Antisense
TGAATGCGCCGCCCAAAAATTCGTT 670 575 422 Antisense
Sequence Source Affymetrix Proprietary Database