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[183225_at]

Description W06B4.2 /REP_DB=WormBase Gene ID /WP=CE02891 /TR=Q23195 /GB=AAC46818.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 20      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 51      6grp_total > 33      4grp_total > 46      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 415.9
(3)
P 		326.9
(-0.1)
P 		247.5
(-2.8)
P 		241
(-3)
P 		175
B 375.7
(3)
P 		318.3
(-0.2)
P 		268.8
(-3)
P 		298.4
(-1.3)
P 		107
C 375
(3)
P 		301.6
(-1.9)
P 		305.1
(-1.7)
P 		286
(-3)
P 		89
avr 388.9
(3) 		315.6
(-0.8) 		273.8
(-3) 		275.1
(-2.9) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 300
1 2 3 4 5 6 range
A 510.7
(0.3)
P 		610.3
(2.4)
P 		541.4
(0.9)
P 		640
(3)
P 		356.5
(-3)
P 		500.1
(0)
P 		284
B 586.9
(2.8)
P 		496
(-0.3)
P 		594.2
(3)
P 		477.5
(-1)
P 		417.8
(-3)
P 		439.8
(-2.3)
P 		176
C 630.7
(2.2)
P 		519.8
(-1.3)
P 		656.3
(3)
P 		501.8
(-1.9)
P 		494.4
(-2.1)
P 		466.4
(-3)
P 		190
avr 576.1
(2.3) 		542
(1.1) 		597.3
(3) 		539.8
(1) 		422.9
(-3) 		468.8
(-1.4) 		174
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 151.4
(3)
P 		71
(-1.2)
P 		64.7
(-1.5)
P 		35.3
(-3)
P 		116
B 119.8
(3)
P 		78.6
(-1.3)
P 		62.3
(-3)
P 		63.6
(-2.9)
P 		58
C 124.2
(3)
P 		86.7
(0)
P 		106.3
(1.6)
P 		48.2
(-3)
P 		76
avr 131.8
(3) 		78.8
(-0.8) 		77.8
(-0.9) 		49
(-3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 140
1 2 3 4 5 6 range
A 94.1
(-3)
P 		217
(3)
P 		117.9
(-1.8)
P 		162.3
(0.3)
P 		107.6
(-2.3)
P 		121.8
(-1.6)
P 		123
B 204.2
(3)
P 		165.1
(-0)
P 		144.3
(-1.6)
P 		126.5
(-3)
P 		169.7
(0.3)
P 		164
(-0.1)
P 		78
C 233.8
(3)
P 		158.6
(-0.4)
P 		167
(-0)
P 		100.9
(-3)
P 		160
(-0.3)
P 		156.1
(-0.5)
P 		133
avr 177.4
(2.7) 		180.2
(3) 		143.1
(-1.4) 		129.9
(-3) 		145.8
(-1.1) 		147.3
(-0.9) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183225_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5255
Sequence Type Exemplar sequence
Representative Public ID W06B4.2 NCBI
Target Description W06B4.2 /REP_DB=WormBase Gene ID /WP=CE02891 /TR=Q23195 /GB=AAC46818.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062388(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062388 NCBI Caenorhabditis elegans W06B4.2 (W06B4.2) mRNA, complete cds. 11/11 None
SNAP00000037000 ENSEMBL cdna:SNAP chromosome:CEL140:II:4468160:4470938:1 11/11 None
GENEFINDER00000037002 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4468160:4470938:1 11/11 None
W06B4.2 ENSEMBL cdna:known chromosome:CEL140:II:4468160:4471024:1 gene:W06B4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4468158-4470937(+) 99.81 100.0
Public Domain and Genome References
Gene Title
Ensembl W06B4.2
Entrez Gene 173784 Entrez gene
SwissProt Q23195 EMBL-EBI
WormBase CE02891 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.17586.1.S1_S_AT similar to N-acetylglucosamine kinase (GlcNAc kinase) cfa
CANINE_2:CFA.18745.1.S1_S_AT similar to N-acetylglucosamine kinase (GlcNAc kinase) cfa
CANINE_2:CFAAFFX.14365.1.S1_AT similar to N-acetylglucosamine kinase (GlcNAc kinase) cfa
DROSOPHILA_2:1629973_AT dm
DROSGENOME1:152123_AT dm
PLASMODIUM_ANOPHELES:218231_AT N-acetylglucosamine kinase hs
HG-U133_PLUS_2:218231_AT N-acetylglucosamine kinase hs
HG-U133A:218231_AT N-acetylglucosamine kinase hs
HG-FOCUS:218231_AT N-acetylglucosamine kinase hs
HG-U133A_2:218231_AT N-acetylglucosamine kinase hs
HU35KSUBA:C02143_S_AT N-acetylglucosamine kinase hs
U133_X3P:G8923736_3P_X_AT N-acetylglucosamine kinase hs
U133_X3P:G8923736_3P_AT N-acetylglucosamine kinase hs
HG-U95B:46246_AT N-acetylglucosamine kinase hs
HG-U95B:55841_AT N-acetylglucosamine kinase hs
HU35KSUBB:RC_AA047270_AT N-acetylglucosamine kinase hs
MG-U74AV2:97385_AT N-acetylglucosamine kinase mm
MG-U74CV2:136116_AT N-acetylglucosamine kinase mm
MG-U74BV2:164541_F_AT N-acetylglucosamine kinase mm
MOE430A:1419509_A_AT N-acetylglucosamine kinase mm
MOUSE430A_2:1419509_A_AT N-acetylglucosamine kinase mm
MOUSE430_2:1419509_A_AT N-acetylglucosamine kinase mm
MOE430B:1438800_AT N-acetylglucosamine kinase mm
MOUSE430_2:1438800_AT N-acetylglucosamine kinase mm
MU19KSUBA:TC20406_S_AT N-acetylglucosamine kinase, mRNA (cDNA clone MGC:5669 IMAGE:3501470) mm
RG-U34C:RC_AI236719_AT N-acetylglucosamine kinase (predicted) rn
RAE230A:1373939_AT N-acetylglucosamine kinase (predicted) rn
RAT230_2:1373939_AT N-acetylglucosamine kinase (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAC46818 Hypothetical protein W06B4.2 [Caenorhabditis elegans] ref|NP_494789.1| W06B4.2 [Caenorhabditis elegans] 0.0
blast CAE67891 Hypothetical protein CBG13487 [Caenorhabditis briggsae] 0.0
blast NP_062415 N-acetylglucosamine kinase [Mus musculus] gb|AAH04689.1| N-acetylglucosamine kinase [Mus musculus] emb|CAB61849.1| N-Acetylglucosamine kinase [Mus musculus] dbj|BAE33171.1| unnamed protein product [Mus musculus] sp|Q9QZ08|NAGK_MOUSE N-acetylglucosamine kinase (GlcNAc kinase) 3.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR002731 EMBL-EBI ATPase, BadF/BadG/BcrA/BcrD type 1.5E-28
Pfam IPR002731 EMBL-EBI ATPase, BadF/BadG/BcrA/BcrD type 1.5E-28
Sequence
Target Sequence 
>C. ELEGANS:183225_AT
tactgattgctgggaccggatcttcgtgtcgactaaaaattcgtgacggatctgtaaaag
gagccggcggctggggacatcaaattggagacggaggaagtgcattttggatcgctcgaa
ctgcgatccagatgcttttcgacgcggaagacgggttcgaggagaactttaatactgatg
ttatcaaacagctgctcttcaaacactataatattactgataaaactagaatgctcgact
atttgtactctaaatttgagaagcataaaattgccgattttacagtttctctcgcggaac
gaactgacgatgcggctgtggcagaagctttcagaagagctggagatgttctcggaaaac
atgtgagaactgtggcaaagtatctcaccaatgaagatcgaaaagttctcagcattgtcc
agatcggaggagtctttcaatcatgggatgctctccaacaaggatttgtggcttctttga
gtggatctggaatcgagaaaatcgtaatgtacgagccatctgattcacctgcggttggcg
cagctgtgctcgctgcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACTGATTGCTGGGACCGGATCTTC 94 647 953 Antisense
GGAAGTGCATTTTGGATCGCTCGAA 565 529 1048 Antisense
TGCGATCCAGATGCTTTTCGACGCG 81 583 1074 Antisense
CAAACAGCTGCTCTTCAAACACTAT 517 191 1137 Antisense
GAATGCTCGACTATTTGTACTCTAA 220 331 1181 Antisense
AATTGCCGATTTTACAGTTTCTCTC 198 179 1221 Antisense
GTTTCTCTCGCGGAACGAACTGACG 479 445 1237 Antisense
GAACTGACGATGCGGCTGTGGCAGA 359 349 1253 Antisense
AGTTCTCAGCATTGTCCAGATCGGA 71 65 1356 Antisense
GCCATCTGATTCACCTGCGGTTGGC 103 281 1467 Antisense
TTGGCGCAGCTGTGCTCGCTGCGAA 667 707 1487 Antisense
Sequence Source Affymetrix Proprietary Database