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[183218_at]

Description T25B6.6 /REP_DB=WormBase Gene ID /WP=CE05016 /TR=Q22761 /GB=AAC48220.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 25      4grp_total > 16      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 15.3
(-3)
A 		17.3
(-2.4)
A 		36.2
(3)
A 		19
(-1.9)
A 		21
B 15.4
(-3)
A 		35.2
(3)
A 		23.2
(-0.6)
A 		27
(0.5)
A 		20
C 19.2
(-2.9)
A 		16.7
(-3)
A 		59.7
(-0.5)
A 		121
(3)
A 		104
avr 16.6
(-3) 		23.1
(-2) 		39.7
(0.5) 		55.7
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 156
1 2 3 4 5 6 range
A 113.1
(3)
A 		69.1
(0.5)
A 		38.5
(-1.2)
A 		12.6
(-2.7)
A 		6.9
(-3)
A 		27.5
(-1.8)
A 		106
B 64
(0.4)
A 		106.8
(3)
M 		9.7
(-3)
A 		10.5
(-3)
A 		10
(-3)
A 		12
(-2.9)
A 		97
C 80.7
(-0.3)
P 		162.9
(3)
M 		77.9
(-0.4)
A 		19.5
(-2.7)
A 		12.8
(-3)
A 		25.2
(-2.5)
A 		150
avr 85.9
(1.4) 		112.9
(3) 		42
(-1.1) 		14.2
(-2.7) 		9.9
(-3) 		21.6
(-2.3) 		103
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 5.6
(-0.5)
A 		3.8
(-2.1)
A 		9.5
(3)
A 		2.8
(-3)
A 		7
B 4.9
(-3)
A 		8.7
(3)
A 		5.4
(-2.2)
A 		5.8
(-1.6)
A 		4
C 6.4
(-2.4)
A 		4.8
(-3)
A 		20.8
(3)
A 		20.4
(2.9)
A 		16
avr 5.6
(-3) 		5.8
(-2.8) 		11.9
(3) 		9.7
(0.9) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 20.8
(2)
A 		24.6
(3)
A 		8.4
(-1.3)
A 		3.2
(-2.7)
A 		2.1
(-3)
A 		6.7
(-1.8)
A 		23
B 22.3
(0.6)
A 		35.6
(3)
M 		2.3
(-3)
A 		2.8
(-2.9)
A 		4.1
(-2.7)
A 		4.5
(-2.6)
A 		33
C 29.9
(0.4)
P 		49.7
(3)
M 		19.8
(-0.9)
A 		3.9
(-3)
A 		4.2
(-3)
A 		8.5
(-2.4)
A 		46
avr 24.3
(0.8) 		36.6
(3) 		10.2
(-1.8) 		3.3
(-3) 		3.5
(-3) 		6.6
(-2.4) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183218_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17036
Sequence Type Exemplar sequence
Representative Public ID T25B6.6 NCBI
Target Description T25B6.6 /REP_DB=WormBase Gene ID /WP=CE05016 /TR=Q22761 /GB=AAC48220.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077124(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077124 NCBI Caenorhabditis elegans T25B6.6 (T25B6.6) mRNA, complete cds. 11/11 None
SNAP00000006428 ENSEMBL cdna:SNAP chromosome:CEL140:X:9020523:9022269:-1 11/11 None
GENEFINDER00000006438 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9021857:9022678:-1 11/11 None
T25B6.6 ENSEMBL cdna:known chromosome:CEL140:X:9021857:9022768:-1 gene:T25B6.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9021855-9022268(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25B6.6
Entrez Gene 181142 Entrez gene
SwissProt Q22761 EMBL-EBI
WormBase CE30191 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4553 hydrolase activity, hydrolyzing O-glycosyl compounds inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_509525 T25B6.6 [Caenorhabditis elegans] gb|AAC48220.2| Hypothetical protein T25B6.6 [Caenorhabditis elegans] 1.0E-57
blast NP_509524 T25B6.5 [Caenorhabditis elegans] gb|AAC48221.1| Hypothetical protein T25B6.5 [Caenorhabditis elegans] 1.0E-48
blast NP_509525 T25B6.6 [Caenorhabditis elegans] gb|AAC48220.2| Hypothetical protein T25B6.6 [Caenorhabditis elegans] 1.0E-36
blast CAE57303 Hypothetical protein CBG00220 [Caenorhabditis briggsae] 7.0E-27
Sequence
Target Sequence 
>C. ELEGANS:183218_AT
atgtcctcgtttctcgtggaaaacggtgcatctcggttctggagagctagtctcgtggaa
aagcttaccgacattcgcccgattgtggaagcaggaaaaaacacaacggtgacgcgtgat
gcccagcaattcgttgcctcaaactacgagcgtctttgcactgtctaccagaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTCCTCGTTTCTCGTGGAAAACG 349 45 13 Antisense
GTGGAAAACGGTGCATCTCGGTTCT 334 487 28 Antisense
GGTGCATCTCGGTTCTGGAGAGCTA 504 501 37 Antisense
GGTTCTGGAGAGCTAGTCTCGTGGA 228 507 47 Antisense
GTCTCGTGGAAAAGCTTACCGACAT 382 453 62 Antisense
TTACCGACATTCGCCCGATTGTGGA 10 681 77 Antisense
CATTCGCCCGATTGTGGAAGCAGGA 352 215 84 Antisense
ACACAACGGTGACGCGTGATGCCCA 626 109 113 Antisense
CCCAGCAATTCGTTGCCTCAAACTA 21 257 134 Antisense
GCCTCAAACTACGAGCGTCTTTGCA 236 283 148 Antisense
CGTCTTTGCACTGTCTACCAGAATA 202 247 163 Antisense
Sequence Source Affymetrix Proprietary Database