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[183215_at]

Description W03A5.3 /REP_DB=WormBase Gene ID /WP=CE21252 /TR=Q23137 /GB=AAD31933.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 50      6grp_cgc > 88      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 233
1 2 3 4 range
A 21.5
(-3)
A 		226.4
(3)
P 		185.5
(1.8)
P 		62.1
(-1.8)
A 		205
B 18.3
(-2.8)
A 		198.3
(2.6)
P 		213
(3)
P 		13.1
(-3)
A 		200
C 51.6
(-2)
A 		244.5
(3)
P 		196.7
(1.8)
P 		12
(-3)
A 		233
avr 30.5
(-3) 		223.1
(3) 		198.4
(2.2) 		29.1
(-3) 		194

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2355
1 2 3 4 5 6 range
A 29.9
(-3)
A 		141.6
(-2.7)
P 		133.8
(-2.7)
P 		1436.8
(0.6)
P 		2366.4
(3)
P 		623.4
(-1.5)
P 		2337
B 43.2
(-3)
A 		156.8
(-2.7)
P 		155.8
(-2.7)
P 		1202.7
(0.3)
P 		2149.7
(3)
P 		608.4
(-1.4)
P 		2107
C 11.3
(-3)
A 		137.5
(-2.7)
P 		109.4
(-2.7)
P 		1188.5
(0.2)
P 		2249
(3)
P 		692.2
(-1.2)
P 		2238
avr 28.1
(-3) 		145.3
(-2.7) 		133
(-2.7) 		1276
(0.4) 		2255
(3) 		641.3
(-1.3) 		2227
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 7.8
(-3)
A 		49.2
(3)
P 		48.5
(2.9)
P 		9.1
(-2.8)
A 		41
B 5.8
(-2.6)
A 		49
(2.9)
P 		49.4
(3)
P 		2.8
(-3)
A 		47
C 17.1
(-1.7)
A 		70.3
(3)
P 		68.5
(2.8)
P 		2
(-3)
A 		68
avr 10.2
(-2.3) 		56.2
(3) 		55.5
(2.9) 		4.6
(-3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 869
1 2 3 4 5 6 range
A 5.5
(-3)
A 		50.4
(-2.6)
P 		29.1
(-2.8)
P 		364.4
(0)
P 		714.1
(3)
P 		151.8
(-1.8)
P 		709
B 15
(-3)
A 		52.2
(-2.7)
P 		37.8
(-2.8)
P 		318.6
(-0.9)
P 		873.4
(3)
P 		226.9
(-1.5)
P 		858
C 4.2
(-3)
A 		41.9
(-2.7)
P 		27.8
(-2.8)
P 		239
(-1.1)
P 		727.7
(3)
P 		231.7
(-1.1)
P 		724
avr 8.2
(-3) 		48.2
(-2.7) 		31.6
(-2.8) 		307.3
(-0.6) 		771.7
(3) 		203.5
(-1.5) 		764
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183215_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6348
Sequence Type Exemplar sequence
Representative Public ID W03A5.3 NCBI
Target Description W03A5.3 /REP_DB=WormBase Gene ID /WP=CE21252 /TR=Q23137 /GB=AAD31933.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065753(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065753 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-22) (grl-22) mRNA, complete cds. 11/11 None
SNAP00000014240 ENSEMBL cdna:SNAP chromosome:CEL140:III:5413179:5413949:-1 11/11 None
GENEFINDER00000014247 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5413179:5413949:-1 11/11 None
W03A5.3 ENSEMBL cdna:known chromosome:CEL140:III:5413179:5413949:-1 gene:W03A5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5413177-5413948(-) 93.46 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-22
Ensembl W03A5.3
Entrez Gene 189140 Entrez gene
SwissProt Q23137 EMBL-EBI
WormBase CE21252 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD31933 Ground-like (grd related) protein 22 [Caenorhabditis elegans] ref|NP_498154.1| GRound-Like (grd related) family member (grl-22) [Caenorhabditis elegans] 8.0E-38
blast E88456 protein W03A5.3 [imported] - Caenorhabditis elegans 8.0E-38
blast CAE61335 Hypothetical protein CBG05173 [Caenorhabditis briggsae] 2.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 2.4E-16
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498154.1 1 5-27
W03A5.3 1 5-27
SNAP00000014240 1 5-27
GENEFINDER00000014247 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:183215_AT
cctgttcccaatggccggaggaggaggtggaggtggtggatgtgctccagcagcaccagc
ctgtgctccaccacctccaccaatgtgcggatgtgcacctcctccaccaccaccgccacc
accaccaatgtgtggatgtggatgcggaagaaagaagagatcagtcgacgatgttgaggg
agttatcaatatggatagtgacgtagagtgcaacaacgaagaattgagagaagttttgga
aaataacatgaaatccaccccttcggactcgcttgtctcagtccgatctaatcttccaac
tgatcagtactttgtaacatgcacccacggactcacagcctactcggctccagctggaac
taaaagttgtgcggttcgcaaggaaagtcacttctgtcaaatcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTGTTCCCAATGGCCGGAGGAGGA 636 265 66 Antisense
CACCTCCACCAATGTGCGGATGTGC 594 201 137 Antisense
CCAATGTGCGGATGTGCACCTCCTC 209 277 145 Antisense
GAGATCAGTCGACGATGTTGAGGGA 365 393 222 Antisense
GGATAGTGACGTAGAGTGCAACAAC 52 509 258 Antisense
AAATAACATGAAATCCACCCCTTCG 286 117 306 Antisense
CTTCGGACTCGCTTGTCTCAGTCCG 11 221 326 Antisense
AGTCCGATCTAATCTTCCAACTGAT 144 61 345 Antisense
CAGTACTTTGTAACATGCACCCACG 238 207 370 Antisense
GTGCGGTTCGCAAGGAAAGTCACTT 685 471 434 Antisense
GGAAAGTCACTTCTGTCAAATCTTC 344 527 447 Antisense
Sequence Source Affymetrix Proprietary Database