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[183204_at]

Description F25E5.3 /REP_DB=WormBase Gene ID /WP=CE07137 /TR=O76653 /GB=AAG24074.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 23      4grp_total > 15      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 67
(0.2)
A 		10.7
(-2.7)
A 		4.4
(-3)
A 		121
(3)
A 		117
B 64.8
(3)
A 		50
(-0.2)
A 		37.2
(-3)
A 		39.1
(-2.6)
A 		28
C 68.2
(3)
A 		25.4
(-3)
A 		57
(1.4)
A 		49.1
(0.3)
A 		43
avr 66.7
(2.6) 		28.7
(-3) 		32.9
(-2.4) 		69.7
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 32.7
(0.3)
A 		45.3
(1.8)
A 		54.9
(3)
A 		21.8
(-1.1)
A 		6.3
(-3)
A 		12.2
(-2.3)
A 		49
B 20.4
(-0.3)
A 		21.8
(-0.1)
A 		20
(-0.4)
A 		39.7
(3)
A 		4.5
(-3)
A 		26.4
(0.7)
A 		35
C 72.8
(3)
A 		33.7
(-0.8)
A 		17.8
(-2.3)
A 		33.3
(-0.8)
A 		10.6
(-3)
A 		11.4
(-2.9)
A 		62
avr 42
(3) 		33.6
(1.6) 		30.9
(1.1) 		31.6
(1.2) 		7.1
(-3) 		16.7
(-1.3) 		35
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 24.4
(3)
A 		2.3
(-2.7)
A 		1.1
(-3)
A 		17.7
(1.3)
A 		23
B 20.7
(3)
A 		12.4
(-1)
A 		8.6
(-2.9)
A 		8.3
(-3)
A 		12
C 22.6
(3)
A 		7.3
(-3)
A 		19.8
(1.9)
A 		8.3
(-2.6)
A 		15
avr 22.6
(3) 		7.3
(-3) 		9.8
(-2) 		11.4
(-1.4) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 6
(-1.3)
A 		16.1
(3)
A 		11.9
(1.2)
A 		5.5
(-1.5)
A 		1.9
(-3)
A 		3
(-2.5)
A 		14
B 7.1
(0.7)
A 		7.3
(0.8)
A 		4.9
(-0.9)
A 		10.5
(3)
A 		1.8
(-3)
A 		9.8
(2.5)
A 		9
C 27
(3)
A 		10.3
(-1.2)
A 		4.5
(-2.7)
A 		6.7
(-2.2)
A 		3.4
(-3)
A 		3.8
(-2.9)
A 		24
avr 13.4
(3) 		11.2
(1.8) 		7.1
(-0.4) 		7.6
(-0.2) 		2.4
(-3) 		5.5
(-1.3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183204_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12102
Sequence Type Exemplar sequence
Representative Public ID F25E5.3 NCBI
Target Description F25E5.3 /REP_DB=WormBase Gene ID /WP=CE07137 /TR=O76653 /GB=AAG24074.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072514(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072514 NCBI Caenorhabditis elegans F25E5.3 (F25E5.3) mRNA, complete cds. 11/11 None
SNAP00000010679 ENSEMBL cdna:SNAP chromosome:CEL140:V:7454313:7455491:1 11/11 None
GENEFINDER00000010693 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7451906:7455491:1 11/11 None
F25E5.3 ENSEMBL cdna:known chromosome:CEL140:V:7454313:7455491:1 gene:F25E5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7454313-7455492(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25E5.3
Entrez Gene 184925 Entrez gene
SwissProt O76653 EMBL-EBI
WormBase CE07137 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG24074 Hypothetical protein F25E5.3 [Caenorhabditis elegans] ref|NP_504915.1| F25E5.3 [Caenorhabditis elegans] 0.0
blast A89102 protein F25E5.4 [imported] - Caenorhabditis elegans 0.0
blast AAG24075 Hypothetical protein F25E5.4 [Caenorhabditis elegans] ref|NP_504914.3| F25E5.4 [Caenorhabditis elegans] 1.0E-172
blast CAE74162 Hypothetical protein CBG21834 [Caenorhabditis briggsae] 2.0E-73
blast CAE57815 Hypothetical protein CBG00839 [Caenorhabditis briggsae] 3.0E-29
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000010693 1 21-43
Sequence
Target Sequence 
>C. ELEGANS:183204_AT
tggagcagcgtgtctaccaaagccaataagtcattccgatattcaagagtttacagtttt
cggattgggaccaaatgagactgcattgacgtctacacggtatgctaaagaagcgtgcga
gcgggagcatgcaggcaattcagtgttttgtggaagaccgttgaaaggaaatagaagatt
gtgtgctggagactttggagctggcgccgttgcaaccattgatggacggaacgttgtttt
gggtatctacgcagaaggtaacactgattgcgatttggaacctgagactcaatcacaagc
ggagtttattgacctaagcgcctacactgaaaaactctgcaggcaaactggaatctgtgt
ggaccagctagaggagacaacagcagagccatctacttccacagacggatactacatttc
tggtgaagagggttcaggcgagagtcagggaactgaaggaactgaacaaccagtcaccac
cgatgcatctatcgaggcaattgtcgctgaaactttcgagaagataaagctcagctgctc
cactgaagccgaaaaagacatccatatccatatccgttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAGCAGCGTGTCTACCAAAGCCA 5 549 531 Antisense
GACTGCATTGACGTCTACACGGTAT 158 375 609 Antisense
GTGCGAGCGGGAGCATGCAGGCAAT 19 479 645 Antisense
TGCTGGAGACTTTGGAGCTGGCGCC 90 583 714 Antisense
GCGCCGTTGCAACCATTGATGGACG 288 287 734 Antisense
AGTTTATTGACCTAAGCGCCTACAC 80 61 833 Antisense
GACAACAGCAGAGCCATCTACTTCC 612 365 906 Antisense
TCACCACCGATGCATCTATCGAGGC 706 625 1004 Antisense
GAGGCAATTGTCGCTGAAACTTTCG 90 405 1024 Antisense
AGATAAAGCTCAGCTGCTCCACTGA 693 65 1052 Antisense
AAGACATCCATATCCATATCCGTTT 25 153 1085 Antisense
Sequence Source Affymetrix Proprietary Database