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[183200_at]

Description F25E5.4 /REP_DB=WormBase Gene ID /WP=CE07138 /TR=O76652 /GB=AAG24075.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 0      4grp_total > 39      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 134.2
(3)
A 		5.4
(-3)
A 		5.7
(-3)
A 		11.7
(-2.7)
A 		129
B 121.9
(3)
A 		5
(-3)
A 		5.3
(-3)
A 		18.3
(-2.3)
A 		117
C 96.2
(3)
A 		6.8
(-2.9)
A 		5.2
(-3)
A 		54.3
(0.2)
A 		91
avr 117.4
(3) 		5.7
(-3) 		5.4
(-3) 		28.1
(-1.8) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 89
1 2 3 4 5 6 range
A 24.8
(-2)
A 		98.1
(3)
A 		10.8
(-3)
A 		60.7
(0.4)
A 		18.2
(-2.5)
A 		75.8
(1.5)
A 		87
B 15
(-3)
A 		17.3
(-2.8)
A 		24.6
(-2.2)
A 		86.1
(3)
A 		44.2
(-0.5)
A 		74.3
(2)
A 		71
C 13.7
(-3)
A 		100
(3)
A 		96
(2.7)
A 		65.5
(0.6)
A 		46.5
(-0.7)
A 		58.8
(0.1)
A 		86
avr 17.8
(-3) 		71.8
(3) 		43.8
(-0.1) 		70.8
(2.9) 		36.3
(-0.9) 		69.6
(2.8) 		54
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 48.9
(3)
A 		1.2
(-3)
A 		1.5
(-3)
A 		1.7
(-2.9)
A 		48
B 38.9
(3)
A 		1.2
(-3)
A 		1.2
(-3)
A 		3.9
(-2.6)
A 		38
C 31.8
(3)
A 		1.9
(-3)
A 		1.8
(-3)
A 		9.2
(-1.5)
A 		30
avr 39.9
(3) 		1.4
(-3) 		1.5
(-3) 		4.9
(-2.5) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 4.6
(-2.6)
A 		34.9
(3)
A 		2.3
(-3)
A 		15.4
(-0.6)
A 		5.5
(-2.4)
A 		18.4
(-0)
A 		33
B 5.2
(-3)
A 		5.7
(-2.9)
A 		6
(-2.8)
A 		22.8
(1.7)
A 		18
(0.4)
A 		27.7
(3)
A 		23
C 5.1
(-3)
A 		30.5
(3)
A 		24.4
(1.6)
A 		13.2
(-1.1)
A 		15.1
(-0.6)
A 		19.7
(0.4)
A 		25
avr 5
(-3) 		23.7
(3) 		10.9
(-1.1) 		17.1
(0.9) 		12.9
(-0.5) 		21.9
(2.4) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183200_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11725
Sequence Type Exemplar sequence
Representative Public ID F25E5.4 NCBI
Target Description F25E5.4 /REP_DB=WormBase Gene ID /WP=CE07138 /TR=O76652 /GB=AAG24075.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072513(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072513 NCBI Caenorhabditis elegans F25E5.4 (F25E5.4) mRNA, complete cds. 11/11 None
SNAP00000010678 ENSEMBL cdna:SNAP chromosome:CEL140:V:7452002:7453749:1 11/11 None
GENEFINDER00000010693 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7451906:7455491:1 11/11 None
F25E5.4 ENSEMBL cdna:known chromosome:CEL140:V:7451945:7453741:1 gene:F25E5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7452002-7453750(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25E5.4
Entrez Gene 179133 Entrez gene
SwissProt O76652 EMBL-EBI
WormBase CE32873 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG24075 Hypothetical protein F25E5.4 [Caenorhabditis elegans] ref|NP_504914.3| F25E5.4 [Caenorhabditis elegans] 0.0
blast A89102 protein F25E5.4 [imported] - Caenorhabditis elegans 0.0
blast AAG24074 Hypothetical protein F25E5.3 [Caenorhabditis elegans] ref|NP_504915.1| F25E5.3 [Caenorhabditis elegans] 0.0
blast AAG24075 Hypothetical protein F25E5.4 [Caenorhabditis elegans] ref|NP_504914.3| F25E5.4 [Caenorhabditis elegans] 1.0E-172
blast A89102 protein F25E5.4 [imported] - Caenorhabditis elegans 1.0E-168
blast CAE57815 Hypothetical protein CBG00839 [Caenorhabditis briggsae] 6.0E-67
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000010693 1 21-43
Sequence
Target Sequence 
>C. ELEGANS:183200_AT
acgggaatttgtactccatcaccaccatcaccagaggagtcaactacgctgtctccaaac
gtgcccgagggaacccttgagcaagaatttttcacagaaaaaccagagtttacatttgac
ccaattattactgccgagtccgagggaacaactccagaagaaatcatcacggaaaatccc
gtgtccacatatgagtctgccagaacaactgaagtaatgttcacggaagtggaaccagaa
gatgaaatcaccgaatcttcagtccgaccaactggagaaagtggtccaggaatgtcttcc
actgggtcaccagaggaagaattcacgagcccttcaccagtggacttcacaacacaatac
ccggatcccactaccactgtgcctgcaattagaattggtgaaaatggagaagttatccac
gctgagacattcgagcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGGGAATTTGTACTCCATCACCAC 442 95 895 Antisense
AGAGGAGTCAACTACGCTGTCTCCA 602 63 927 Antisense
GTGCCCGAGGGAACCCTTGAGCAAG 358 477 955 Antisense
GACCCAATTATTACTGCCGAGTCCG 134 381 1012 Antisense
CGTGTCCACATATGAGTCTGCCAGA 429 245 1074 Antisense
AATCTTCAGTCCGACCAACTGGAGA 220 171 1148 Antisense
CAGGAATGTCTTCCACTGGGTCACC 239 209 1181 Antisense
AGGAAGAATTCACGAGCCCTTCACC 71 55 1208 Antisense
CCTTCACCAGTGGACTTCACAACAC 46 269 1225 Antisense
CACTACCACTGTGCCTGCAATTAGA 618 197 1263 Antisense
TATCCACGCTGAGACATTCGAGCCA 491 657 1308 Antisense
Sequence Source Affymetrix Proprietary Database