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[183197_at]

Description T25C12.2 /REP_DB=WormBase Gene ID /WP=CE03735 /TR=Q22776 /GB=CAA91486.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 65      4grp_total > 05      6grp_cgc > 57      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 71.6
(-0.7)
A 		6.1
(-3)
A 		6.8
(-3)
A 		175.5
(3)
A 		169
B 38.9
(-1.4)
A 		22.4
(-2.2)
A 		5.1
(-3)
A 		134.2
(3)
P 		129
C 89.3
(0.3)
A 		12.9
(-2.8)
A 		6.9
(-3)
A 		155.7
(3)
P 		149
avr 66.6
(-0.6) 		13.8
(-2.7) 		6.3
(-3) 		155.1
(3) 		149

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 232
1 2 3 4 5 6 range
A 9.3
(-2.8)
A 		2.3
(-3)
A 		115.2
(0.1)
P 		65.4
(-1.3)
A 		47.9
(-1.8)
A 		221.5
(3)
P 		219
B 13
(-3)
A 		35.5
(-2.2)
A 		137.4
(1.4)
A 		81.1
(-0.6)
A 		50.9
(-1.6)
A 		181.2
(3)
P 		168
C 46.1
(-2.5)
A 		30.2
(-2.9)
A 		164.3
(1)
P 		180.8
(1.4)
A 		28
(-3)
A 		234.6
(3)
P 		207
avr 22.8
(-3) 		22.7
(-3) 		139
(0.7) 		109.1
(-0.3) 		42.3
(-2.4) 		212.4
(3) 		190
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 26.1
(3)
A 		1.3
(-3)
A 		1.8
(-2.9)
A 		25.7
(2.9)
A 		25
B 12.4
(-0.5)
A 		5.5
(-2.1)
A 		1.2
(-3)
A 		28.6
(3)
P 		27
C 29.6
(3)
A 		3.7
(-2.7)
A 		2.4
(-3)
A 		26.3
(2.3)
P 		27
avr 22.7
(2) 		3.5
(-2.6) 		1.8
(-3) 		26.9
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 1.7
(-2.9)
A 		0.8
(-3)
A 		25.1
(-0.3)
P 		16.6
(-1.2)
A 		14.4
(-1.5)
A 		53.9
(3)
P 		53
B 4.5
(-3)
A 		11.8
(-2.3)
A 		33.4
(-0.3)
A 		21.5
(-1.4)
A 		20.7
(-1.5)
A 		67.6
(3)
P 		63
C 17.1
(-2.3)
A 		9.2
(-3)
A 		41.8
(-0.2)
P 		36.4
(-0.6)
A 		9.1
(-3)
A 		78.5
(3)
P 		69
avr 7.8
(-2.9) 		7.3
(-3) 		33.4
(-0.4) 		24.8
(-1.2) 		14.7
(-2.3) 		66.7
(3) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183197_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16887
Sequence Type Exemplar sequence
Representative Public ID T25C12.2 NCBI
Target Description T25C12.2 /REP_DB=WormBase Gene ID /WP=CE03735 /TR=Q22776 /GB=CAA91486.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077517, NM_001029756
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000017731 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11487718:11490416:1 11/11 None
T25C12.2 ENSEMBL cdna:known chromosome:CEL140:X:11487718:11490416:1 gene:T25C12.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029756 6/11 Cross Hyb Matching Probes None
NM_077517 5/11 Cross Hyb Matching Probes None
SNAP00000017726 5/11 Cross Hyb Matching Probes None
SNAP00000017728 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11487717-11490416(+) 100.0 100.0
Public Domain and Genome References
Ensembl T25C12.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAI91169 Hypothetical protein T25C12.4 [Caenorhabditis elegans] ref|NP_001024927.1| SaPosin-like Protein family member (spp-21) [Caenorhabditis elegans] 1.0E-43
blast CAI91169 Hypothetical protein T25C12.4 [Caenorhabditis elegans] ref|NP_001024927.1| SaPosin-like Protein family member (spp-21) [Caenorhabditis elegans] 2.0E-43
blast CAA91486 Hypothetical protein T25C12.2 [Caenorhabditis elegans] ref|NP_509918.2| SaPosin-like Protein family member (spp-9) [Caenorhabditis elegans] 8.0E-39
blast CAA91486 Hypothetical protein T25C12.2 [Caenorhabditis elegans] ref|NP_509918.2| SaPosin-like Protein family member (spp-9) [Caenorhabditis elegans] 1.0E-38
blast CAE70633 Hypothetical protein CBG17325 [Caenorhabditis briggsae] 1.0E-29
blast CAE70633 Hypothetical protein CBG17325 [Caenorhabditis briggsae] 2.0E-29
Protein Domains
Database ID Description E-Value
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 8.8E-6
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 8.8E-6
Sequence
Target Sequence 
>C. ELEGANS:183197_AT
tctctttctggttgcggttgttgctgttaccactgctttagttttccctgaacagagagc
aagtgcgtttgactgtttcttgtgccgtttggctgttaacaccaccgatccccctgtcga
caacgagctccatatcgttgaagataaattccttgccgaatgtaagaaagaactcaaagg
aatcccttttttggagcagacttgtctaaactacgcccactcggagttcgatccgatcat
caaggagctcgaatctggaactgcaccagaagatgacacaatgaaaactgttctatttct
ggttgccgttgttgctattgccactgctttagttttccctgaacagagggctagtgcgtt
tgactgtttcttgtgccgtttggctgttaagaccgccgatccctttgtggacgaagagtt
ccatattgctgaagataaatttctcgccgaatgcaaaaaagaactcaaaggaattccctt
tttggagcagacatgtctgaactttgcccactcggagttcggtccaatcatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCTTTCTGGTTGCGGTTGTTGCT 196 611 24 Antisense
GCTGTTACCACTGCTTTAGTTTTCC 548 293 46 Antisense
CGTTTGGCTGTTAACACCACCGATC 120 247 109 Antisense
AGCAGACTTGTCTAAACTACGCCCA 61 77 218 Antisense
AGGAGCTCGAATCTGGAACTGCACC 110 57 266 Antisense
TTCTGGTTGCCGTTGTTGCTATTGC 663 691 320 Antisense
GCTATTGCCACTGCTTTAGTTTTCC 313 303 337 Antisense
GAGGGCTAGTGCGTTTGACTGTTTC 449 401 369 Antisense
GTTTGGCTGTTAAGACCGCCGATCC 552 451 401 Antisense
GCAGACATGTCTGAACTTTGCCCAC 499 313 510 Antisense
CCACTCGGAGTTCGGTCCAATCATC 625 273 531 Antisense
Sequence Source Affymetrix Proprietary Database