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[183190_at]

Description C54E4.5 /REP_DB=WormBase Gene ID /WP=CE16947 /TR=O44467 /GB=AAK29798.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 18      6grp_high1_10_10 > 81      4grp_highest_8_8 > 03      4grp_high_8_8 > 46      6grp_highest_nonorm > 54      6grp_total > 82      4grp_total > 41      6grp_highest_sub > 04_05      6grp_high1_sub > 04      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 618
1 2 3 4 range
A 536.3
(-3)
P 		1105.4
(3)
P 		1067.8
(2.6)
P 		877.5
(0.6)
P 		569
B 675
(-2.4)
P 		1109.1
(2.5)
P 		1154.2
(3)
P 		621.6
(-3)
P 		533
C 554.7
(-3)
P 		1021
(3)
P 		954.2
(2.1)
P 		773.8
(-0.2)
P 		466
avr 588.7
(-3) 		1078.5
(3) 		1058.7
(2.8) 		757.6
(-0.9) 		490

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1873
1 2 3 4 5 6 range
A 840.3
(-1.3)
P 		903.5
(-1.1)
P 		1076.4
(-0.6)
P 		2193.6
(3)
P 		1592.7
(1.1)
P 		323.4
(-3)
P 		1870
B 773
(-1.8)
P 		865.7
(-1.5)
P 		915.3
(-1.3)
P 		1932.2
(2.1)
P 		2195.9
(3)
P 		405.9
(-3)
P 		1790
C 750.3
(-1.6)
P 		815.8
(-1.3)
P 		1267.4
(0.5)
P 		1631.9
(2)
P 		1880.1
(3)
P 		408.8
(-3)
P 		1471
avr 787.9
(-1.4) 		861.7
(-1.1) 		1086.4
(-0.2) 		1919.2
(3) 		1889.6
(2.9) 		379.4
(-3) 		1540
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 195.3
(-0.3)
P 		240
(1.4)
P 		279.2
(3)
P 		128.5
(-3)
P 		151
B 215.2
(0.5)
P 		273.9
(3)
P 		267.4
(2.7)
P 		132.5
(-3)
P 		141
C 183.6
(-1.4)
P 		293.5
(1.8)
P 		332.6
(3)
P 		130.5
(-3)
P 		202
avr 198
(-0.5) 		269.1
(2.1) 		293.1
(3) 		130.5
(-3) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 813
1 2 3 4 5 6 range
A 154.9
(-2)
P 		321.3
(0)
P 		234.4
(-1)
P 		556.4
(3)
P 		480.6
(2)
P 		78.7
(-3)
P 		478
B 269
(-2)
P 		288.2
(-1.9)
P 		222.3
(-2.4)
P 		511.8
(-0.1)
P 		892.1
(3)
P 		151.4
(-3)
P 		741
C 278.1
(-1.2)
P 		248.9
(-1.6)
P 		322.6
(-0.6)
P 		328.2
(-0.6)
P 		608.4
(3)
P 		136.8
(-3)
P 		472
avr 234
(-1.8) 		286.1
(-1.2) 		259.8
(-1.5) 		465.5
(0.8) 		660.4
(3) 		122.3
(-3) 		538
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183190_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10847
Sequence Type Exemplar sequence
Representative Public ID C54E4.5 NCBI
Target Description C54E4.5 /REP_DB=WormBase Gene ID /WP=CE16947 /TR=O44467 /GB=AAK29798.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067769(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067769 NCBI Caenorhabditis elegans C54E4.5 (C54E4.5) mRNA, complete cds. 11/11 None
GENEFINDER00000038605 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2146719:2149295:-1 11/11 None
C54E4.5 ENSEMBL cdna:known chromosome:CEL140:IV:2148013:2149295:-1 gene:C54E4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2148018-2152012(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C54E4.5
Entrez Gene 183790 Entrez gene
SwissProt Q65CM5 EMBL-EBI
WormBase CE37495 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE65566 Hypothetical protein CBG10559 [Caenorhabditis briggsae] 1.0E-148
blast AAU87825 Hypothetical protein C54E4.5 [Caenorhabditis elegans] ref|NP_500170.2| C54E4.5 [Caenorhabditis elegans] 1.0E-114
blast E88642 protein C54E4.5 [imported] - Caenorhabditis elegans 1.0E-114
blast AAU87825 Hypothetical protein C54E4.5 [Caenorhabditis elegans] ref|NP_500170.2| C54E4.5 [Caenorhabditis elegans] 1.0E-113
blast E88642 protein C54E4.5 [imported] - Caenorhabditis elegans 1.0E-113
blast CAE65566 Hypothetical protein CBG10559 [Caenorhabditis briggsae] 1.0E-101
Protein Domains
Database ID Description E-Value
scop a.3.1.Two-domain cytochrome c All alpha proteins; Cytochrome c; Cytochrome c; Two-domain cytochrome c 2.90000009536743
Pfam IPR007828 EMBL-EBI Protein of unknown function DUF706 1.7E-92
Pfam IPR007828 EMBL-EBI Protein of unknown function DUF706 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:183190_AT
gacgtcaaggctgaggacccgattcaagttcgtgtcaggacccattatttcacccagcac
cagaagcagacagtggattttgtaaaggaaatgcatcaaaagtggctgaaattcgaccac
gcgaaaatgccaattctcggctgcctggacatgctagccacatttttagacgaatctgat
ccggatgtggatgaggcaaacttaattcatgcttaccagacagctgaaaaaatcagagaa
aatcacccggacaagccgtggatgcatttggcaggtctaatccacgacttggggaaaatc
atgagcgtatggggtgaagagcaatgggcagtcaccggtgacacgtatcctgttggttgt
gcaccagccgcctctattgtctacggaaaatccagcttcgatgggaacccggacattagc
catccggtatacggtacccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGTCAAGGCTGAGGACCCGATTC 102 379 460 Antisense
GATTCAAGTTCGTGTCAGGACCCAT 76 429 480 Antisense
TCACCCAGCACCAGAAGCAGACAGT 307 627 509 Antisense
TGCCTGGACATGCTAGCCACATTTT 560 585 601 Antisense
ATCACCCGGACAAGCCGTGGATGCA 42 29 701 Antisense
TTGGCAGGTCTAATCCACGACTTGG 443 707 727 Antisense
GAAGAGCAATGGGCAGTCACCGGTG 351 339 775 Antisense
TCACCGGTGACACGTATCCTGTTGG 128 627 791 Antisense
AGCCGCCTCTATTGTCTACGGAAAA 703 81 825 Antisense
AATCCAGCTTCGATGGGAACCCGGA 635 165 848 Antisense
TAGCCATCCGGTATACGGTACCCAA 428 649 876 Antisense
Sequence Source Affymetrix Proprietary Database