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[183183_s_at]

Description F33D4.4 /REP_DB=WormBase Gene ID /WP=CE17034 /TR=O44186 /GB=AAB88372.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 71      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 65      6grp_total > 70      4grp_total > 47      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 653
1 2 3 4 range
A 1403.8
(3)
P 		876
(-3)
P 		933.8
(-2.3)
P 		996.7
(-1.6)
P 		528
B 1349.8
(3)
P 		826
(-2.3)
P 		759.8
(-3)
P 		860
(-2)
P 		590
C 1179.8
(3)
P 		852.5
(-1.6)
P 		859.4
(-1.5)
P 		750.5
(-3)
P 		429
avr 1311.1
(3) 		851.5
(-3) 		851
(-3) 		869.1
(-2.8) 		460

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 542
1 2 3 4 5 6 range
A 911.6
(-3)
P 		933.7
(-2.7)
P 		1002.9
(-1.8)
P 		1369.2
(3)
P 		1144
(0)
P 		1008.4
(-1.7)
P 		458
B 929.4
(-3)
P 		1130
(0.6)
P 		1036.1
(-1.1)
P 		1261.6
(3)
P 		1007.6
(-1.6)
P 		963.5
(-2.4)
P 		332
C 1058.9
(-0.6)
P 		1128.1
(0.2)
P 		1177.2
(0.7)
P 		1383.9
(3)
P 		1014
(-1.1)
P 		841.6
(-3)
P 		542
avr 966.6
(-2.6) 		1063.9
(-1.1) 		1072.1
(-1) 		1338.2
(3) 		1055.2
(-1.2) 		937.8
(-3) 		400
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 385
1 2 3 4 range
A 511.1
(3)
P 		190.2
(-2.3)
P 		244.1
(-1.4)
P 		145.9
(-3)
P 		365
B 430.5
(3)
P 		204
(-2.3)
P 		176.1
(-3)
P 		183.3
(-2.8)
P 		254
C 390.5
(3)
P 		245.1
(-0.3)
P 		299.5
(0.9)
P 		126.6
(-3)
P 		264
avr 444
(3) 		213.1
(-1.7) 		239.9
(-1.2) 		151.9
(-3) 		292

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 241
1 2 3 4 5 6 range
A 168
(-3)
P 		332.1
(2.5)
P 		218.4
(-1.3)
P 		347.3
(3)
P 		345.2
(2.9)
P 		245.5
(-0.4)
P 		179
B 323.4
(-0.3)
P 		376.1
(1.7)
P 		251.6
(-3)
P 		334.2
(0.1)
P 		409.4
(3)
P 		359.3
(1.1)
P 		158
C 392.5
(3)
P 		344.2
(0.5)
P 		299.6
(-1.9)
P 		278.3
(-3)
P 		328.1
(-0.4)
P 		281.7
(-2.8)
P 		114
avr 294.6
(-0.8) 		350.8
(2.4) 		256.5
(-3) 		319.9
(0.6) 		360.9
(3) 		295.5
(-0.8) 		104
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183183_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9302
Sequence Type Exemplar sequence
Representative Public ID F33D4.4 NCBI
Target Description F33D4.4 /REP_DB=WormBase Gene ID /WP=CE17034 /TR=O44186 /GB=AAB88372.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068855(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068855 NCBI Caenorhabditis elegans F33D4.4 (F33D4.4) mRNA, complete cds. 11/11 None
SNAP00000032723 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7704192:7706886:-1 11/11 None
GENEFINDER00000032736 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7704192:7706886:-1 11/11 None
F33D4.4.2 ENSEMBL cdna:known chromosome:CEL140:IV:7703872:7706889:-1 gene:F33D4.4 11/11 None
F33D4.4.1 ENSEMBL cdna:known chromosome:CEL140:IV:7703872:7706888:-1 gene:F33D4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7704197-7706892(-) 98.43 100.0
Public Domain and Genome References
Gene Title
Ensembl F33D4.4
Entrez Gene 177548 Entrez gene
SwissProt O44186 EMBL-EBI
WormBase CE17034 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:255276_AT fatty acid desaturase family protein at
CANINE:1586012_AT similar to degenerative spermatocyte homolog 1, lipid desaturase cfa
CANINE_2:CFA.12492.1.S1_AT similar to degenerative spermatocyte homolog 1, lipid desaturase cfa
CANINE_2:CFAAFFX.25085.1.S1_S_AT similar to degenerative spermatocyte homolog 1, lipid desaturase cfa
DROSOPHILA_2:1625414_AT infertile crescent dm
DROSGENOME1:153842_AT infertile crescent dm
CHICKEN:GGA.8162.1.S1_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) gga
HG-U133_PLUS_2:207431_S_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133A:207431_S_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133A_2:207431_S_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133_PLUS_2:209250_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133A:209250_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-FOCUS:209250_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133A_2:209250_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HU35KSUBA:AF002668_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HU35KSUBD:RC_AA186666_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
U133_X3P:G4505192_3P_A_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
U133_X3P:G12803018_3P_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U95AV2:33337_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
MU11KSUBB:Y08460_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MU11KSUBB:Y08460_G_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MG-U74AV2:95688_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOE430A:1423345_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOUSE430A_2:1423345_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOUSE430_2:1423345_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOE430A:1423346_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOUSE430A_2:1423346_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOUSE430_2:1423346_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MU19KSUBA:TC20045_AT Degenerative spermatocyte homolog 1 (Drosophila), mRNA (cDNA clone MGC:5864 IMAGE:3600571) mm
MU19KSUBC:TC39637_S_AT Degenerative spermatocyte homolog 1 (Drosophila), mRNA (cDNA clone MGC:5864 IMAGE:3600571) mm
RG-U34C:RC_AI177412_AT degenerative spermatocyte homolog (Drosophila) rn
RAE230A:1367770_AT degenerative spermatocyte homolog (Drosophila) rn
RAT230_2:1367770_AT degenerative spermatocyte homolog (Drosophila) rn
RAE230B:1381384_AT Degenerative spermatocyte homolog (Drosophila) rn
RAT230_2:1381384_AT Degenerative spermatocyte homolog (Drosophila) rn
RAE230B:1386022_AT Degenerative spermatocyte homolog (Drosophila) rn
RAT230_2:1386022_AT Degenerative spermatocyte homolog (Drosophila) rn
RAE230B:1391406_AT Degenerative spermatocyte homolog (Drosophila) rn
RAT230_2:1391406_AT Degenerative spermatocyte homolog (Drosophila) rn
RG-U34C:RC_AI169572_AT Degenerative spermatocyte homolog (Drosophila) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB88372 Hypothetical protein F33D4.4 [Caenorhabditis elegans] ref|NP_501256.1| F33D4.4 [Caenorhabditis elegans] 0.0
blast CAE61942 Hypothetical protein CBG05940 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 2.3E-49
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 8.3E-45
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501256.1 5 43-62,67-89,104-126,186-205,210-232
F33D4.4.2 5 43-62,67-89,104-126,186-205,210-232
F33D4.4.1 5 43-62,67-89,104-126,186-205,210-232
SNAP00000032723 5 43-62,67-89,104-126,186-205,210-232
GENEFINDER00000032736 5 43-62,67-89,104-126,186-205,210-232
Sequence
Target Sequence 
>C. ELEGANS:183183_S_AT
tggatgagtcttcaaccacttttctatggaatccgtccattcatgctttatccaaaatca
atgactgatttggagttgattaatgttgcaattcaacttttattcagtacatttatctac
acacaattcggagcaaaatcatttttctttttgttcggaggacttgttcttggaatggga
ttgcatccatgtgctggtcactttgtttccgaacactacgttttcaagaaggatcaggaa
acttacagttattacggtccaatcaatatggtagttttcaatgtcggatatcacgtcgaa
catcacgatttcccttacataactggatcaaatcttccaaaggtccgcgagattgctcct
gaatactaccaggattggcagactcatgacagttgggttggtatgatggccgacttcatt
tttaatccaaaaatgaccctccgcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGATGAGTCTTCAACCACTTTTCT 367 549 499 Antisense
TGGAATCCGTCCATTCATGCTTTAT 588 545 525 Antisense
TTTGTTCGGAGGACTTGTTCTTGGA 193 411 648 Antisense
GATTGCATCCATGTGCTGGTCACTT 94 435 677 Antisense
GGTCACTTTGTTTCCGAACACTACG 149 501 694 Antisense
ACATCACGATTTCCCTTACATAACT 15 107 798 Antisense
GATCAAATCTTCCAAAGGTCCGCGA 451 421 824 Antisense
GCGAGATTGCTCCTGAATACTACCA 53 291 845 Antisense
ACCAGGATTGGCAGACTCATGACAG 169 85 866 Antisense
GATGGCCGACTTCATTTTTAATCCA 623 405 903 Antisense
TAATCCAAAAATGACCCTCCGCAAA 138 635 921 Antisense
Sequence Source Affymetrix Proprietary Database