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[183179_at]

Description F30B5.6 /REP_DB=WormBase Gene ID /WP=CE04467 /TR=Q19912 /GB=AAA83495.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 38      4grp_total > 12      6grp_cgc > 04      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 17.7
(-3)
A 		54.2
(1.5)
A 		19.5
(-2.8)
A 		66.5
(3)
P 		49
B 46.1
(3)
A 		5.5
(-3)
A 		22
(-0.6)
A 		38.6
(1.9)
A 		41
C 27.7
(-1.7)
A 		10.7
(-2.6)
A 		3.9
(-3)
A 		111.8
(3)
P 		108
avr 30.5
(-1.4) 		23.5
(-2.1) 		15.1
(-3) 		72.3
(3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 33.9
(3)
A 		14.2
(-3)
A 		15.1
(-2.7)
A 		24.1
(0)
A 		14.4
(-2.9)
A 		16.8
(-2.2)
A 		20
B 30.6
(3)
A 		29.6
(2.8)
A 		1.8
(-3)
A 		15.1
(-0.2)
A 		25.6
(2)
A 		18.1
(0.4)
A 		29
C 6.3
(-2.2)
A 		35.2
(3)
A 		4.7
(-2.5)
A 		2.1
(-3)
A 		18.3
(-0.1)
A 		20.1
(0.3)
P 		33
avr 23.6
(2.2) 		26.3
(3) 		7.2
(-3) 		13.8
(-0.9) 		19.4
(0.8) 		18.3
(0.5) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 6.4
(-1.8)
A 		11.8
(3)
A 		5.1
(-3)
A 		9.7
(1.1)
P 		7
B 14.7
(3)
A 		1.4
(-3)
A 		5.1
(-1.3)
A 		8.2
(0.1)
A 		13
C 9.2
(-0.3)
A 		3.1
(-2.4)
A 		1.3
(-3)
A 		18.9
(3)
P 		18
avr 10.1
(1.4) 		5.4
(-1.9) 		3.8
(-3) 		12.3
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 6.3
(3)
A 		5.1
(0.6)
A 		3.3
(-3)
A 		6.1
(2.6)
A 		4.3
(-1)
A 		4.1
(-1.4)
A 		3
B 10.7
(3)
A 		9.8
(2.5)
A 		0.4
(-3)
A 		4
(-0.9)
A 		10.4
(2.8)
A 		6.8
(0.7)
A 		10
C 2.3
(-1.9)
A 		10.7
(3)
A 		1.2
(-2.5)
A 		0.4
(-3)
A 		5.9
(0.2)
A 		6.7
(0.7)
P 		10
avr 6.4
(1.2) 		8.5
(3) 		1.6
(-3) 		3.5
(-1.3) 		6.9
(1.6) 		5.9
(0.7) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183179_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10859
Sequence Type Exemplar sequence
Representative Public ID F30B5.6 NCBI
Target Description F30B5.6 /REP_DB=WormBase Gene ID /WP=CE04467 /TR=Q19912 /GB=AAA83495.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068115(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068115 NCBI Caenorhabditis elegans Serpentine Receptor, class BC (class B-like) family member (srbc-70) (srbc-70) mRNA, complete cds. 11/11 None
GENEFINDER00000023161 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4226052:4231838:-1 10/11 None
F30B5.6 ENSEMBL cdna:known chromosome:CEL140:IV:4226851:4228010:-1 gene:F30B5.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027991 3/11 Cross Hyb Matching Probes None
SNAP00000023154 3/11 Cross Hyb Matching Probes None
SNAP00000023155 8/11 Cross Hyb Matching Probes None
F30B5.8 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4226856-4228016(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srbc-70
Ensembl F30B5.6
Entrez Gene 185128 Entrez gene
SwissProt Q19912 EMBL-EBI
WormBase CE04467 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_500516 Serpentine Receptor, class BC (class B-like) family member (srbc-70) [Caenorhabditis elegans] gb|AAA83495.1| Hypothetical protein F30B5.6 [Caenorhabditis elegans] 1.0E-158
blast NP_500517 F30B5.7 [Caenorhabditis elegans] gb|AAA83498.2| Hypothetical protein F30B5.7 [Caenorhabditis elegans] 1.0E-135
blast NP_001023162 Serpentine Receptor, class BC (class B-like) family member (srbc-71) [Caenorhabditis elegans] gb|AAW88401.1| Hypothetical protein F30B5.8 [Caenorhabditis elegans] 1.0E-121
blast NP_500516 Serpentine Receptor, class BC (class B-like) family member (srbc-70) [Caenorhabditis elegans] gb|AAA83495.1| Hypothetical protein F30B5.6 [Caenorhabditis elegans] 1.0E-110
blast NP_001023162 Serpentine Receptor, class BC (class B-like) family member (srbc-71) [Caenorhabditis elegans] gb|AAW88401.1| Hypothetical protein F30B5.8 [Caenorhabditis elegans] 1.0E-85
blast CAB07629 Hypothetical protein F54B8.9 [Caenorhabditis elegans] ref|NP_506954.1| Serpentine Receptor, class BC (class B-like) family member (srbc-51) [Caenorhabditis elegans] 6.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 1.1E-96
Pfam IPR003689 EMBL-EBI Zinc transporter ZIP 4.0E-35
Pfam IPR003689 EMBL-EBI Zinc transporter ZIP 1.3E-9
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 4.1E-86
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500516.1 7 10-32,44-66,81-103,124-146,178-197,216-233,243-265
F30B5.6 7 10-32,44-66,81-103,124-146,178-197,216-233,243-265
GENEFINDER00000023161 11 10-30,43-65,85-102,288-310,330-352,365-382,420-442,463-485,518-537,550-572,587-609
Sequence
Target Sequence 
>C. ELEGANS:183179_AT
gatattctccatagcgttgtttcgatttatcgctatatacttccaaatttcatatcacaa
caatagccataaaactctactctgtgcaatttccgtcaatctgtgtctattaatattttt
cgatcaatatatgatgtttggatattgcaataatgtcattgatgttccgttagtgtgtga
taattctaaatgtgcatataatcagtgttatttcgatttttggatgtttcgagaaaagct
ggtttatatttgtatttcaatgttatcagcagttcttgccattcgacttttcgtctggaa
gttctgcaccaaaaatagaactgatcacatgttttctaaggccacacaaattgctcttct
agactctgctgtcgtaatattctccaacattctacctgtattcgtgtcgtctcaattatc
caacatcgactttttaatcacttcctccatgaccactgtgtgcaggaatttgggacttct
gataagtactgtgttaatttatagaatattcatgagagataagaagttagtgtctgttct
gatggtaactccttcagctaggcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATTCTCCATAGCGTTGTTTCGA 7 427 306 Antisense
AAAACTCTACTCTGTGCAATTTCCG 109 131 376 Antisense
GCAATTTCCGTCAATCTGTGTCTAT 378 323 391 Antisense
GTTATCAGCAGTTCTTGCCATTCGA 658 445 567 Antisense
TTGCCATTCGACTTTTCGTCTGGAA 542 699 581 Antisense
CAAATTGCTCTTCTAGACTCTGCTG 654 193 652 Antisense
ACTCTGCTGTCGTAATATTCTCCAA 471 99 668 Antisense
TTCTACCTGTATTCGTGTCGTCTCA 173 691 695 Antisense
CATCGACTTTTTAATCACTTCCTCC 46 213 729 Antisense
TCCTCCATGACCACTGTGTGCAGGA 91 593 748 Antisense
TGATGGTAACTCCTTCAGCTAGGCC 418 553 845 Antisense
Sequence Source Affymetrix Proprietary Database