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[183146_at]

Description B0554.6 /REP_DB=WormBase Gene ID /WP=CE07778 /TR=P91002 /GB=AAB37611.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 33      4grp_total > 58      6grp_cgc > 80      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 58
(0.9)
A 		29.9
(-3)
A 		73.1
(3)
A 		50.4
(-0.2)
A 		43
B 52.7
(-1.8)
A 		103.8
(3)
A 		65.9
(-0.6)
A 		39.8
(-3)
A 		64
C 53.6
(0.1)
A 		66.1
(1.4)
A 		81.7
(3)
A 		24
(-3)
A 		58
avr 54.8
(-0.2) 		66.6
(1.8) 		73.6
(3) 		38.1
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 46.5
(1.4)
A 		21.2
(-1.7)
A 		59.4
(3)
A 		10.7
(-3)
A 		21.6
(-1.7)
A 		35.8
(0.1)
A 		49
B 46.9
(1.3)
A 		63.4
(3)
A 		26.8
(-0.9)
A 		47.9
(1.4)
A 		6.8
(-3)
A 		12.8
(-2.4)
A 		57
C 41.2
(0.8)
A 		9.5
(-2.7)
A 		61.2
(3)
A 		18.7
(-1.7)
A 		39.5
(0.6)
A 		6.9
(-3)
A 		54
avr 44.9
(2.2) 		31.4
(-0.5) 		49.1
(3) 		25.8
(-1.6) 		22.6
(-2.2) 		18.5
(-3) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 21.1
(3)
A 		6.5
(-3)
A 		19.1
(2.2)
A 		7.4
(-2.6)
A 		15
B 16.8
(-0.1)
A 		25.6
(3)
A 		15.3
(-0.6)
A 		8.5
(-3)
A 		17
C 17.7
(0.4)
A 		19
(0.7)
A 		28.5
(3)
A 		4
(-3)
A 		25
avr 18.5
(2) 		17
(1.3) 		21
(3) 		6.6
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 8.6
(0.5)
A 		7.5
(-0.2)
A 		12.9
(3)
A 		2.7
(-3)
A 		6.5
(-0.8)
A 		8.7
(0.5)
A 		10
B 16.3
(1.4)
A 		21.1
(3)
A 		6.5
(-1.8)
A 		12.7
(0.2)
A 		2.8
(-3)
A 		4.8
(-2.3)
A 		18
C 15.3
(2.9)
A 		2.9
(-2.7)
A 		15.6
(3)
A 		3.8
(-2.3)
A 		12.8
(1.7)
A 		2.3
(-3)
A 		13
avr 13.4
(3) 		10.5
(0.9) 		11.7
(1.7) 		6.4
(-2.2) 		7.4
(-1.4) 		5.3
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183146_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15798
Sequence Type Exemplar sequence
Representative Public ID B0554.6 NCBI
Target Description B0554.6 /REP_DB=WormBase Gene ID /WP=CE07778 /TR=P91002 /GB=AAB37611.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070795(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070795 NCBI Caenorhabditis elegans Downstream Of DAF-16 (regulated by DAF-16) family member (dod-20) (dod-20) mRNA, complete cds. 11/11 None
SNAP00000032181 ENSEMBL cdna:SNAP chromosome:CEL140:V:422390:423893:1 11/11 None
GENEFINDER00000032191 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:422327:426385:1 11/11 None
B0554.6 ENSEMBL cdna:known chromosome:CEL140:V:422327:423995:1 gene:B0554.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:422326-423995(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dod-20
Ensembl B0554.6
Entrez Gene 182037 Entrez gene
SwissProt P91002 EMBL-EBI
WormBase CE07778 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37611 Downstream of daf-16 (regulated by daf-16) protein 20 [Caenorhabditis elegans] ref|NP_503196.1| Downstream Of DAF-16 (regulated by DAF-16) family member (dod-20) [Caenorhabditis elegans] 0.0
blast AAB37612 Hypothetical protein B0554.5 [Caenorhabditis elegans] ref|NP_503197.1| B0554.5 [Caenorhabditis elegans] 0.0
blast AAQ91895 Hypothetical protein Y39D8A.1d [Caenorhabditis elegans] ref|NP_001024232.1| Y39D8A.1d [Caenorhabditis elegans] pir||T33784 hypothetical protein Y39D8A.1 - Caenorhabditis elegans 0.0
blast AAG45577 Hypothetical protein ZK6.11a [Caenorhabditis elegans] ref|NP_001024314.1| ZK6.11a [Caenorhabditis elegans] 1.0E-120
blast AAT68887 Hypothetical protein ZK6.11b [Caenorhabditis elegans] ref|NP_001024315.1| ZK6.11b [Caenorhabditis elegans] 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 1.0E-126
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 1.0E-126
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 1.1E-58
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000032191 12 381-403,410-427,432-454,467-489,519-541,580-602,612-634,647-669,689-711,723-742,747-769,795-814
Sequence
Target Sequence 
>C. ELEGANS:183146_AT
gccttcaccactactggattctatatgaagcccctggccagaaatgctagtaccataact
gttaatatcaagcgagatgttagatattctggagcttctggtgcaaatcttctcggacat
gtcccaaagcggggaaaagtcacagtaggcttttataacggagattcaaaatatgaagaa
tcattccctccaagttactatttctctccatggagcatcccatatattgactttgaaact
ttcaaagtttcatccacatcctcaaactcaactgctgaatactactttgtgcaatatttt
gtcgctcaaggtactctgttcggtagtccaacttcagggcccattcagacggcaactcaa
tcctccggagtcgtccaattattcgtgtcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCTTCACCACTACTGGATTCTATA 209 281 742 Antisense
GCCCCTGGCCAGAAATGCTAGTACC 610 285 771 Antisense
GGAGCTTCTGGTGCAAATCTTCTCG 230 521 832 Antisense
ATCATTCCCTCCAAGTTACTATTTC 470 29 921 Antisense
ATTTCTCTCCATGGAGCATCCCATA 98 17 941 Antisense
TTTCAAAGTTTCATCCACATCCTCA 667 671 981 Antisense
CACATCCTCAAACTCAACTGCTGAA 24 197 996 Antisense
TACTCTGTTCGGTAGTCCAACTTCA 215 645 1053 Antisense
CAACTTCAGGGCCCATTCAGACGGC 656 187 1070 Antisense
ATTCAGACGGCAACTCAATCCTCCG 267 11 1084 Antisense
GGAGTCGTCCAATTATTCGTGTCAA 417 515 1108 Antisense
Sequence Source Affymetrix Proprietary Database