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[183142_at]

Description B0348.2 /REP_DB=WormBase Gene ID /WP=CE17327 /TR=O61958 /GB=AAC17809.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 92      6grp_highest_nonorm > 72      6grp_total > 91      4grp_total > 23      6grp_highest_sub > 04_05      6grp_high1_sub > 04_05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 199
1 2 3 4 range
A 276.9
(1.9)
P 		149
(-3)
A 		217.8
(-0.3)
P 		304.5
(3)
P 		156
B 348.4
(3)
P 		194.6
(-2.2)
M 		171
(-3)
P 		259.2
(-0)
P 		177
C 273.4
(1.1)
P 		250
(0.2)
M 		168.5
(-3)
P 		322.5
(3)
P 		154
avr 299.6
(3) 		197.9
(-2.4) 		185.8
(-3) 		295.4
(2.8) 		114

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 692
1 2 3 4 5 6 range
A 269.9
(-1.7)
P 		121
(-3)
P 		176.9
(-2.5)
P 		812.5
(3)
P 		642.4
(1.5)
P 		183.2
(-2.5)
P 		692
B 398.4
(-0.7)
P 		148.1
(-3)
P 		270.1
(-1.9)
P 		794.5
(3)
P 		696.8
(2.1)
P 		228.3
(-2.3)
P 		646
C 299.7
(-1.5)
P 		163.6
(-3)
P 		219.4
(-2.4)
P 		706.3
(3)
P 		579.8
(1.6)
P 		234.9
(-2.2)
P 		543
avr 322.7
(-1.3) 		144.2
(-3) 		222.1
(-2.3) 		771.1
(3) 		639.7
(1.7) 		215.5
(-2.3) 		627
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 100.8
(3)
P 		32.3
(-3)
A 		56.9
(-0.8)
P 		44.6
(-1.9)
P 		69
B 111.1
(3)
P 		48.1
(-2.3)
M 		39.6
(-3)
P 		55.2
(-1.7)
P 		72
C 90.5
(3)
P 		71.9
(-0.1)
M 		58.7
(-2.3)
P 		54.4
(-3)
P 		36
avr 100.8
(3) 		50.8
(-3) 		51.7
(-2.9) 		51.4
(-2.9) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 49.7
(-2.6)
P 		43
(-2.8)
P 		38.5
(-3)
P 		206.1
(3)
P 		193.8
(2.6)
P 		44.6
(-2.8)
P 		168
B 138.6
(-0.7)
P 		49.3
(-3)
P 		65.6
(-2.6)
P 		210.4
(1.1)
P 		283.1
(3)
P 		85.1
(-2.1)
P 		234
C 111.1
(-0.3)
P 		49.9
(-3)
P 		55.8
(-2.7)
P 		142
(1)
P 		187.6
(3)
P 		78.6
(-1.7)
P 		138
avr 99.8
(-1.2) 		47.4
(-3) 		53.3
(-2.8) 		186.2
(1.8) 		221.5
(3) 		69.4
(-2.2) 		174
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183142_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15702
Sequence Type Exemplar sequence
Representative Public ID B0348.2 NCBI
Target Description B0348.2 /REP_DB=WormBase Gene ID /WP=CE17327 /TR=O61958 /GB=AAC17809.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070726(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070726 NCBI Caenorhabditis elegans B0348.2 (B0348.2) mRNA, complete cds. 11/11 None
GENEFINDER00000028748 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:30660:47150:1 9/11 None
B0348.2 ENSEMBL cdna:known chromosome:CEL140:V:45295:46708:1 gene:B0348.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028743 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:45294-46877(+) 60.52 64.54
Public Domain and Genome References
Gene Title
Ensembl B0348.2
Entrez Gene 181936 Entrez gene
SwissProt O61958 EMBL-EBI
WormBase CE32297 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL77167 Egg laying defective protein 8, isoform a [Caenorhabditis elegans] ref|NP_001021346.1| EGg Laying defective family member (egl-8) [Caenorhabditis elegans] 0.0
blast AAF01458 phospholipase C beta [Caenorhabditis elegans] 0.0
blast AAL77168 Egg laying defective protein 8, isoform b [Caenorhabditis elegans] ref|NP_001021347.1| EGg Laying defective family member (egl-8) [Caenorhabditis elegans] gb|AAF05701.1| phospholipase C beta homolog EGL-8 [Caenorhabditis elegans] 0.0
blast AAC17809 Hypothetical protein B0348.2 [Caenorhabditis elegans] ref|NP_503127.2| B0348.2 [Caenorhabditis elegans] 2.0E-47
blast AAC17810 Hypothetical protein B0348.1 [Caenorhabditis elegans] ref|NP_503128.1| B0348.1 [Caenorhabditis elegans] 1.0E-29
blast CAE62305 Hypothetical protein CBG06367 [Caenorhabditis briggsae] 2.0E-26
Protein Domains
Database ID Description E-Value
scop a.2.5.Prefoldin All alpha proteins; Long alpha-hairpin; Prefoldin; Prefoldin 9.80000019073486
Pfam IPR009535 EMBL-EBI Protein of unknown function DUF1154 1.4E-36
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 3.2E-70
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503127.2 1 40-62
B0348.2 1 40-62
GENEFINDER00000028748 1 863-885
Sequence
Target Sequence 
>C. ELEGANS:183142_AT
gttgttttaggacctacgctcactgaatgcaccgagccgcatcaggtgttttgccaaaaa
tgccagtgcaatcaagtgacacgtactgagacacaacttggcgcctgctggtgggttgtc
tttatcattggatgcctcttgttctggccaatctgctactggctttgctgtgattcatcc
aaggatacgatgcattattgcccgtcatgcggaactttgttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGTTTTAGGACCTACGCTCACTG 64 437 28 Antisense
TACGCTCACTGAATGCACCGAGCCG 40 647 42 Antisense
GCACCGAGCCGCATCAGGTGTTTTG 415 315 56 Antisense
ATGCCAGTGCAATCAAGTGACACGT 699 39 87 Antisense
ACGTACTGAGACACAACTTGGCGCC 345 95 108 Antisense
GCTGGTGGGTTGTCTTTATCATTGG 7 295 134 Antisense
TCATTGGATGCCTCTTGTTCTGGCC 321 619 152 Antisense
TGTTCTGGCCAATCTGCTACTGGCT 335 563 167 Antisense
CTGGCTTTGCTGTGATTCATCCAAG 456 241 186 Antisense
GATACGATGCATTATTGCCCGTCAT 145 425 211 Antisense
TGCCCGTCATGCGGAACTTTGTTGG 599 583 226 Antisense
Sequence Source Affymetrix Proprietary Database